Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage

Abstract The compositional and functional role of the endophytic bacterial community, associated with black scented rice, in correlation with its antioxidant property has been elucidated. Community dissimilarity analysis confirmed the overlapping of community in shoot and root tissues at the young s...

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Autores principales:	K. Malabika Singha, Brahmanand Singh, Piyush Pandey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba5ae57eee4c41bcb1b8b9102fd0b952
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spelling	oai:doaj.org-article:ba5ae57eee4c41bcb1b8b9102fd0b9522021-12-02T17:47:04ZHost specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage10.1038/s41598-021-91452-42045-2322https://doaj.org/article/ba5ae57eee4c41bcb1b8b9102fd0b9522021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91452-4https://doaj.org/toc/2045-2322Abstract The compositional and functional role of the endophytic bacterial community, associated with black scented rice, in correlation with its antioxidant property has been elucidated. Community dissimilarity analysis confirmed the overlapping of community in shoot and root tissues at the young stage, but not in mature plants. Proteobacteria was the most abundant phylum, in which Agrobacterium, Pleomorphomonas, Bradyrhizobium, Novasphingobium, Caulobacter were the most abundant genera, followed by Cyanobacteria and Planctomycetes in all three different varieties of the black rice. The antioxidant activity of mature plants was found to be higher in comparison to young plants. Intrinsically, the relative abundance of Pleomorphomonas and Streptomyces was positively correlated with total phenol content, while Gemmata, unclassified Pirellulaceae, unclassified Stramenopiles positively correlated with total flavonoid content and negatively correlated with Free radical scavenging activity. Accordingly, functional metagenome analysis of the endophytic microbiome revealed that naringenin -3-dioxygenase and anthocyanidin 3-O-glucosyltransferase for phenylpropanoid (flavonoid and anthocyanin) synthesis were abundant in the endophytic microbiome of mature plants. Specific enrichment of the antioxidant producing genes in the mature plant endophytic microbiome was assigned to some bacteria such as Streptomyces, Pantoea which might have contributed to the common pathway of flavonoid synthesis. The genomes of endophytic isolates Kluyvera sp.PO2S7, Bacillus subtilis AMR1 and Enterobacter sp. SES19 were sequenced and annotated, and were found to have genes for phenylpropanoid synthesis in their genomes.K. Malabika SinghaBrahmanand SinghPiyush PandeyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q K. Malabika Singha Brahmanand Singh Piyush Pandey Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage
description	Abstract The compositional and functional role of the endophytic bacterial community, associated with black scented rice, in correlation with its antioxidant property has been elucidated. Community dissimilarity analysis confirmed the overlapping of community in shoot and root tissues at the young stage, but not in mature plants. Proteobacteria was the most abundant phylum, in which Agrobacterium, Pleomorphomonas, Bradyrhizobium, Novasphingobium, Caulobacter were the most abundant genera, followed by Cyanobacteria and Planctomycetes in all three different varieties of the black rice. The antioxidant activity of mature plants was found to be higher in comparison to young plants. Intrinsically, the relative abundance of Pleomorphomonas and Streptomyces was positively correlated with total phenol content, while Gemmata, unclassified Pirellulaceae, unclassified Stramenopiles positively correlated with total flavonoid content and negatively correlated with Free radical scavenging activity. Accordingly, functional metagenome analysis of the endophytic microbiome revealed that naringenin -3-dioxygenase and anthocyanidin 3-O-glucosyltransferase for phenylpropanoid (flavonoid and anthocyanin) synthesis were abundant in the endophytic microbiome of mature plants. Specific enrichment of the antioxidant producing genes in the mature plant endophytic microbiome was assigned to some bacteria such as Streptomyces, Pantoea which might have contributed to the common pathway of flavonoid synthesis. The genomes of endophytic isolates Kluyvera sp.PO2S7, Bacillus subtilis AMR1 and Enterobacter sp. SES19 were sequenced and annotated, and were found to have genes for phenylpropanoid synthesis in their genomes.
format	article
author	K. Malabika Singha Brahmanand Singh Piyush Pandey
author_facet	K. Malabika Singha Brahmanand Singh Piyush Pandey
author_sort	K. Malabika Singha
title	Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage
title_short	Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage
title_full	Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage
title_fullStr	Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage
title_full_unstemmed	Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage
title_sort	host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ba5ae57eee4c41bcb1b8b9102fd0b952
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Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage

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