MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish

Abstract Gametogenesis is a complicated biological process by which sperm and egg are produced for genetic transmission between generations. In many animals, the germline is segregated from the somatic lineage in early embryonic development through the specification of primordial germ cells (PGCs),...

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Autores principales:	Jing Zhang, Wei Liu, Yilin Jin, Peng Jia, Kuntong Jia, Meisheng Yi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba5ca10f514340579ef59afbca235e3f
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spelling	oai:doaj.org-article:ba5ca10f514340579ef59afbca235e3f2021-12-02T15:06:07ZMiR-202-5p is a novel germ plasm-specific microRNA in zebrafish10.1038/s41598-017-07675-x2045-2322https://doaj.org/article/ba5ca10f514340579ef59afbca235e3f2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07675-xhttps://doaj.org/toc/2045-2322Abstract Gametogenesis is a complicated biological process by which sperm and egg are produced for genetic transmission between generations. In many animals, the germline is segregated from the somatic lineage in early embryonic development through the specification of primordial germ cells (PGCs), the precursors of gametes for reproduction and fertility. In some species, such as fruit fly and zebrafish, PGCs are determined by the maternally provided germ plasm which contains various RNAs and proteins. Here, we identified a germ plasm/PGC-specific microRNA miR-202-5p for the first time in zebrafish. MiR-202-5p was specifically expressed in gonad. In female, it was expressed and accumulated in oocytes during oogenesis. Quantitative reverse transcription PCR and whole mount in situ hybridization results indicated that miR-202-5p exhibited a typical germ plasm /PGC-specific expression pattern throughout embryogenesis, which was consistent with that of the PGC marker vasa, indicating that miR-202-5p was a component of germ plasm and a potential PGC marker in zebrafish. Our present study might be served as a foundation for further investigating the regulative roles of miRNAs in germ plasm formation and PGC development in zebrafish and other teleost.Jing ZhangWei LiuYilin JinPeng JiaKuntong JiaMeisheng YiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Zhang Wei Liu Yilin Jin Peng Jia Kuntong Jia Meisheng Yi MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish
description	Abstract Gametogenesis is a complicated biological process by which sperm and egg are produced for genetic transmission between generations. In many animals, the germline is segregated from the somatic lineage in early embryonic development through the specification of primordial germ cells (PGCs), the precursors of gametes for reproduction and fertility. In some species, such as fruit fly and zebrafish, PGCs are determined by the maternally provided germ plasm which contains various RNAs and proteins. Here, we identified a germ plasm/PGC-specific microRNA miR-202-5p for the first time in zebrafish. MiR-202-5p was specifically expressed in gonad. In female, it was expressed and accumulated in oocytes during oogenesis. Quantitative reverse transcription PCR and whole mount in situ hybridization results indicated that miR-202-5p exhibited a typical germ plasm /PGC-specific expression pattern throughout embryogenesis, which was consistent with that of the PGC marker vasa, indicating that miR-202-5p was a component of germ plasm and a potential PGC marker in zebrafish. Our present study might be served as a foundation for further investigating the regulative roles of miRNAs in germ plasm formation and PGC development in zebrafish and other teleost.
format	article
author	Jing Zhang Wei Liu Yilin Jin Peng Jia Kuntong Jia Meisheng Yi
author_facet	Jing Zhang Wei Liu Yilin Jin Peng Jia Kuntong Jia Meisheng Yi
author_sort	Jing Zhang
title	MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish
title_short	MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish
title_full	MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish
title_fullStr	MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish
title_full_unstemmed	MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish
title_sort	mir-202-5p is a novel germ plasm-specific microrna in zebrafish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ba5ca10f514340579ef59afbca235e3f
work_keys_str_mv	AT jingzhang mir2025pisanovelgermplasmspecificmicrornainzebrafish AT weiliu mir2025pisanovelgermplasmspecificmicrornainzebrafish AT yilinjin mir2025pisanovelgermplasmspecificmicrornainzebrafish AT pengjia mir2025pisanovelgermplasmspecificmicrornainzebrafish AT kuntongjia mir2025pisanovelgermplasmspecificmicrornainzebrafish AT meishengyi mir2025pisanovelgermplasmspecificmicrornainzebrafish
_version_	1718388606189436928

MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish

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