Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study

Magnesium (Mg) alloys that have both antibacterial and osteogenic properties are suitable candidates for orthopedic implants. However, the fabrication of ideal Mg implants suitable for bone repair remains challenging because it requires implants with interconnected pore structures and personalized g...

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Autores principales:	Kai Xie, Nanqing Wang, Yu Guo, Shuang Zhao, Jia Tan, Lei Wang, Guoyuan Li, Junxiang Wu, Yangzi Yang, Wenyu Xu, Juan Chen, Wenbo Jiang, Penghuai Fu, Yongqiang Hao
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Magnesium implants 3D printing Implant-related infections Antibacterial activity Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ba5e1d99c293423ba0a74841132805a8
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spelling	oai:doaj.org-article:ba5e1d99c293423ba0a74841132805a82021-11-04T04:36:13ZAdditively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study2452-199X10.1016/j.bioactmat.2021.06.032https://doaj.org/article/ba5e1d99c293423ba0a74841132805a82022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21003182https://doaj.org/toc/2452-199XMagnesium (Mg) alloys that have both antibacterial and osteogenic properties are suitable candidates for orthopedic implants. However, the fabrication of ideal Mg implants suitable for bone repair remains challenging because it requires implants with interconnected pore structures and personalized geometric shapes. In this study, we fabricated a porous 3D-printed Mg-Nd-Zn-Zr (denoted as JDBM) implant with suitable mechanical properties using selective laser melting technology. The 3D-printed JDBM implant exhibited cytocompatibility in MC3T3-E1 and RAW267.4 cells and excellent osteoinductivity in vitro. Furthermore, the implant demonstrated excellent antibacterial ratios of 90.0% and 92.1% for methicillin-resistant S. aureus (MRSA) and Escherichia coli, respectively. The 3D-printed JDBM implant prevented MRSA-induced implant-related infection in a rabbit model and showed good in vivo biocompatibility based on the results of histological evaluation, blood tests, and Mg2+ deposition detection. In addition, enhanced inflammatory response and TNF-α secretion were observed at the bone-implant interface of the 3D-printed JDBM implants during the early implantation stage. The high Mg2+ environment produced by the degradation of 3D-printed JDBM implants could promote M1 phenotype of macrophages (Tnf, iNOS, Ccl3, Ccl4, Ccl5, Cxcl10, and Cxcl2), and enhance the phagocytic ability of macrophages. The enhanced immunoregulatory effect generated by relatively fast Mg2+ release and implant degradation during the early implantation stage is a potential antibacterial mechanism of Mg-based implant. Our findings indicate that 3D-printed porous JDBM implants, having both antibacterial property and osteoinductivity, hold potential for future orthopedic applications.Kai XieNanqing WangYu GuoShuang ZhaoJia TanLei WangGuoyuan LiJunxiang WuYangzi YangWenyu XuJuan ChenWenbo JiangPenghuai FuYongqiang HaoKeAi Communications Co., Ltd.articleMagnesium implants3D printingImplant-related infectionsAntibacterial activityMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 8, Iss , Pp 140-152 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Magnesium implants 3D printing Implant-related infections Antibacterial activity Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Magnesium implants 3D printing Implant-related infections Antibacterial activity Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Kai Xie Nanqing Wang Yu Guo Shuang Zhao Jia Tan Lei Wang Guoyuan Li Junxiang Wu Yangzi Yang Wenyu Xu Juan Chen Wenbo Jiang Penghuai Fu Yongqiang Hao Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study
description	Magnesium (Mg) alloys that have both antibacterial and osteogenic properties are suitable candidates for orthopedic implants. However, the fabrication of ideal Mg implants suitable for bone repair remains challenging because it requires implants with interconnected pore structures and personalized geometric shapes. In this study, we fabricated a porous 3D-printed Mg-Nd-Zn-Zr (denoted as JDBM) implant with suitable mechanical properties using selective laser melting technology. The 3D-printed JDBM implant exhibited cytocompatibility in MC3T3-E1 and RAW267.4 cells and excellent osteoinductivity in vitro. Furthermore, the implant demonstrated excellent antibacterial ratios of 90.0% and 92.1% for methicillin-resistant S. aureus (MRSA) and Escherichia coli, respectively. The 3D-printed JDBM implant prevented MRSA-induced implant-related infection in a rabbit model and showed good in vivo biocompatibility based on the results of histological evaluation, blood tests, and Mg2+ deposition detection. In addition, enhanced inflammatory response and TNF-α secretion were observed at the bone-implant interface of the 3D-printed JDBM implants during the early implantation stage. The high Mg2+ environment produced by the degradation of 3D-printed JDBM implants could promote M1 phenotype of macrophages (Tnf, iNOS, Ccl3, Ccl4, Ccl5, Cxcl10, and Cxcl2), and enhance the phagocytic ability of macrophages. The enhanced immunoregulatory effect generated by relatively fast Mg2+ release and implant degradation during the early implantation stage is a potential antibacterial mechanism of Mg-based implant. Our findings indicate that 3D-printed porous JDBM implants, having both antibacterial property and osteoinductivity, hold potential for future orthopedic applications.
format	article
author	Kai Xie Nanqing Wang Yu Guo Shuang Zhao Jia Tan Lei Wang Guoyuan Li Junxiang Wu Yangzi Yang Wenyu Xu Juan Chen Wenbo Jiang Penghuai Fu Yongqiang Hao
author_facet	Kai Xie Nanqing Wang Yu Guo Shuang Zhao Jia Tan Lei Wang Guoyuan Li Junxiang Wu Yangzi Yang Wenyu Xu Juan Chen Wenbo Jiang Penghuai Fu Yongqiang Hao
author_sort	Kai Xie
title	Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study
title_short	Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study
title_full	Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study
title_fullStr	Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study
title_full_unstemmed	Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study
title_sort	additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: an in vitro and in vivo study
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/ba5e1d99c293423ba0a74841132805a8
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Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study

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