Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles

China M Kummitha, Anthony S Malamas, Zheng-Rong LuDepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USAAbstract: Nonspecific association of serum molecules with short-interfering RNA (siRNA) nanoparticles can change their physiochemical characteristics, and result...

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Autores principales:	Kummitha CM, Malamas AS, Lu ZR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ba661cf827764c5ea12acce67e137075
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spelling	oai:doaj.org-article:ba661cf827764c5ea12acce67e1370752021-12-02T01:08:09ZAlbumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles1176-91141178-2013https://doaj.org/article/ba661cf827764c5ea12acce67e1370752012-10-01T00:00:00Zhttp://www.dovepress.com/albumin-pre-coating-enhances-intracellular-sirna-delivery-of-multifunc-a11161https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013China M Kummitha, Anthony S Malamas, Zheng-Rong LuDepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USAAbstract: Nonspecific association of serum molecules with short-interfering RNA (siRNA) nanoparticles can change their physiochemical characteristics, and results in reduced cellular uptake in the target tissue during the systemic siRNA delivery process. Serum albumin is the most abundant protein in the body and has been used to modify the surface of nanoparticles, to inhibit association of other serum molecules. Here, we hypothesized that surface modification of lipid-based nanoparticular siRNA delivery systems with albumin could prevent their interaction with serum proteins, and improve intracellular uptake. In this study, we investigated the influence of albumin on the stability and intracellular siRNA delivery of the targeted siRNA nanoparticles of a polymerizable and pH-sensitive multifunctional surfactant N-(1-aminoethyl)iminobis[N-(oleoylcysteinylhistinyl-1-aminoethyl)propionamide] (EHCO) in serum. Serum resulted in a significant increase in the size of targeted EHCO/siRNA nanoparticles and inhibited cellular uptake of the nanoparticles. Coating of targeted EHCO/siRNA nanoparticles with bovine serum albumin at 9.4 &micro;M prior to cell transfection improved cellular uptake and gene silencing efficacy of EHCO/siRNA targeted nanoparticles in serum-containing media, as compared with the uncoated nanoparticles. At a proper concentration, albumin has the potential to minimize interactions of serum proteins with siRNA nanoparticles for effective systemic in vivo siRNA delivery.Keywords: multifunctional, lipid nanoparticles, RNA interference, pH-sensitive amphiphile, siRNAKummitha CMMalamas ASLu ZRDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5205-5214 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kummitha CM Malamas AS Lu ZR Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles
description	China M Kummitha, Anthony S Malamas, Zheng-Rong LuDepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USAAbstract: Nonspecific association of serum molecules with short-interfering RNA (siRNA) nanoparticles can change their physiochemical characteristics, and results in reduced cellular uptake in the target tissue during the systemic siRNA delivery process. Serum albumin is the most abundant protein in the body and has been used to modify the surface of nanoparticles, to inhibit association of other serum molecules. Here, we hypothesized that surface modification of lipid-based nanoparticular siRNA delivery systems with albumin could prevent their interaction with serum proteins, and improve intracellular uptake. In this study, we investigated the influence of albumin on the stability and intracellular siRNA delivery of the targeted siRNA nanoparticles of a polymerizable and pH-sensitive multifunctional surfactant N-(1-aminoethyl)iminobis[N-(oleoylcysteinylhistinyl-1-aminoethyl)propionamide] (EHCO) in serum. Serum resulted in a significant increase in the size of targeted EHCO/siRNA nanoparticles and inhibited cellular uptake of the nanoparticles. Coating of targeted EHCO/siRNA nanoparticles with bovine serum albumin at 9.4 &micro;M prior to cell transfection improved cellular uptake and gene silencing efficacy of EHCO/siRNA targeted nanoparticles in serum-containing media, as compared with the uncoated nanoparticles. At a proper concentration, albumin has the potential to minimize interactions of serum proteins with siRNA nanoparticles for effective systemic in vivo siRNA delivery.Keywords: multifunctional, lipid nanoparticles, RNA interference, pH-sensitive amphiphile, siRNA
format	article
author	Kummitha CM Malamas AS Lu ZR
author_facet	Kummitha CM Malamas AS Lu ZR
author_sort	Kummitha CM
title	Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles
title_short	Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles
title_full	Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles
title_fullStr	Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles
title_full_unstemmed	Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles
title_sort	albumin pre-coating enhances intracellular sirna delivery of multifunctional amphiphile/sirna nanoparticles
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/ba661cf827764c5ea12acce67e137075
work_keys_str_mv	AT kummithacm albuminprecoatingenhancesintracellularsirnadeliveryofmultifunctionalamphiphilesirnananoparticles AT malamasas albuminprecoatingenhancesintracellularsirnadeliveryofmultifunctionalamphiphilesirnananoparticles AT luzr albuminprecoatingenhancesintracellularsirnadeliveryofmultifunctionalamphiphilesirnananoparticles
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Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles

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