Expression of HLA class I and HLA class II by tumor cells in Chinese classical Hodgkin lymphoma patients.

<h4>Background</h4>In Caucasian populations, the tumor cells of Epstein Barr virus (EBV)-positive classical Hodgkin Lymphomas (cHL) patients more frequently express HLA class I and HLA class II molecules compared to EBV-negative cHL patients. HLA expression (in relation to EBV) in Asian...

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Autores principales:	Xin Huang, Anke van den Berg, Zifen Gao, Lydia Visser, Ilja Nolte, Hans Vos, Bouke Hepkema, Wierd Kooistra, Sibrand Poppema, Arjan Diepstra
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba737e2fc1ce43788ae47bcab2cd9ed9
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spelling	oai:doaj.org-article:ba737e2fc1ce43788ae47bcab2cd9ed92021-12-02T20:21:18ZExpression of HLA class I and HLA class II by tumor cells in Chinese classical Hodgkin lymphoma patients.1932-620310.1371/journal.pone.0010865https://doaj.org/article/ba737e2fc1ce43788ae47bcab2cd9ed92010-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20526359/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>In Caucasian populations, the tumor cells of Epstein Barr virus (EBV)-positive classical Hodgkin Lymphomas (cHL) patients more frequently express HLA class I and HLA class II molecules compared to EBV-negative cHL patients. HLA expression (in relation to EBV) in Asian cHL patients has not been previously investigated.<h4>Methodology/principal findings</h4>We randomly selected 145 cHL patients with formalin-fixed, paraffin embedded tissue blocks available from 5 hospitals from the Northern part of China. Hematoxylin & Eosin-stained slides were used to re-classify the histological subtypes according to the WHO classification. EBV status was determined by visualization of EBERs in tumor cells using in situ hybridization. Membranous expression of HLA molecules was detected by immunohistochemistry using antibodies HC-10 (class I heavy chain) and anti-beta2-microglobulin for HLA class I, and CR3/43 for HLA class II. EBV+ tumor cells were observed in 40% (58/145) of the cHL patients. As expected, the percentage of EBV+ cases was much higher in the mixed cellularity subtype (71%) than in the nodular sclerosis subtype (16%) (p<0.001). Expression of HLA class I was observed in 79% of the EBV+ cHL cases and in 30% of the EBV-cases (p<0.001). For HLA class II, 52% of EBV+ cHL cases were positive, compared to 43% in EBV- cases (p = 0.28).<h4>Conclusions</h4>The results in the Northern China population were similar to those in the Caucasian population for HLA class I, but not for HLA class II.Xin HuangAnke van den BergZifen GaoLydia VisserIlja NolteHans VosBouke HepkemaWierd KooistraSibrand PoppemaArjan DiepstraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 5, p e10865 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xin Huang Anke van den Berg Zifen Gao Lydia Visser Ilja Nolte Hans Vos Bouke Hepkema Wierd Kooistra Sibrand Poppema Arjan Diepstra Expression of HLA class I and HLA class II by tumor cells in Chinese classical Hodgkin lymphoma patients.
description	<h4>Background</h4>In Caucasian populations, the tumor cells of Epstein Barr virus (EBV)-positive classical Hodgkin Lymphomas (cHL) patients more frequently express HLA class I and HLA class II molecules compared to EBV-negative cHL patients. HLA expression (in relation to EBV) in Asian cHL patients has not been previously investigated.<h4>Methodology/principal findings</h4>We randomly selected 145 cHL patients with formalin-fixed, paraffin embedded tissue blocks available from 5 hospitals from the Northern part of China. Hematoxylin & Eosin-stained slides were used to re-classify the histological subtypes according to the WHO classification. EBV status was determined by visualization of EBERs in tumor cells using in situ hybridization. Membranous expression of HLA molecules was detected by immunohistochemistry using antibodies HC-10 (class I heavy chain) and anti-beta2-microglobulin for HLA class I, and CR3/43 for HLA class II. EBV+ tumor cells were observed in 40% (58/145) of the cHL patients. As expected, the percentage of EBV+ cases was much higher in the mixed cellularity subtype (71%) than in the nodular sclerosis subtype (16%) (p<0.001). Expression of HLA class I was observed in 79% of the EBV+ cHL cases and in 30% of the EBV-cases (p<0.001). For HLA class II, 52% of EBV+ cHL cases were positive, compared to 43% in EBV- cases (p = 0.28).<h4>Conclusions</h4>The results in the Northern China population were similar to those in the Caucasian population for HLA class I, but not for HLA class II.
format	article
author	Xin Huang Anke van den Berg Zifen Gao Lydia Visser Ilja Nolte Hans Vos Bouke Hepkema Wierd Kooistra Sibrand Poppema Arjan Diepstra
author_facet	Xin Huang Anke van den Berg Zifen Gao Lydia Visser Ilja Nolte Hans Vos Bouke Hepkema Wierd Kooistra Sibrand Poppema Arjan Diepstra
author_sort	Xin Huang
title	Expression of HLA class I and HLA class II by tumor cells in Chinese classical Hodgkin lymphoma patients.
title_short	Expression of HLA class I and HLA class II by tumor cells in Chinese classical Hodgkin lymphoma patients.
title_full	Expression of HLA class I and HLA class II by tumor cells in Chinese classical Hodgkin lymphoma patients.
title_fullStr	Expression of HLA class I and HLA class II by tumor cells in Chinese classical Hodgkin lymphoma patients.
title_full_unstemmed	Expression of HLA class I and HLA class II by tumor cells in Chinese classical Hodgkin lymphoma patients.
title_sort	expression of hla class i and hla class ii by tumor cells in chinese classical hodgkin lymphoma patients.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/ba737e2fc1ce43788ae47bcab2cd9ed9
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Expression of HLA class I and HLA class II by tumor cells in Chinese classical Hodgkin lymphoma patients.

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