Transplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington’s disease

Abstract Background Human embryonic stem cells (hESCs) transplantation had shown to provide a potential source of cells in neurodegenerative disease studies and lead to behavioral recovery in lentivirus transfected or, toxin-induced Huntington's disease (HD) rodent model. Here, we aimed to obse...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jaisan Islam, Kyoung Ha So, Elina KC, Hyeong Cheol Moon, Aryun Kim, Sang Hwan Hyun, Soochong Kim, Young Seok Park
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Adeno-associated virus Huntington's disease Human embryonic stem cell Nanoparticles Cell tracking Medicine (General) R5-920 Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/ba7465a9291745fab8ea1a111bf002d5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ba7465a9291745fab8ea1a111bf002d5
record_format	dspace
spelling	oai:doaj.org-article:ba7465a9291745fab8ea1a111bf002d52021-11-28T12:06:25ZTransplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington’s disease10.1186/s13287-021-02653-71757-6512https://doaj.org/article/ba7465a9291745fab8ea1a111bf002d52021-11-01T00:00:00Zhttps://doi.org/10.1186/s13287-021-02653-7https://doaj.org/toc/1757-6512Abstract Background Human embryonic stem cells (hESCs) transplantation had shown to provide a potential source of cells in neurodegenerative disease studies and lead to behavioral recovery in lentivirus transfected or, toxin-induced Huntington's disease (HD) rodent model. Here, we aimed to observe if transplantation of superparamagnetic iron oxide nanoparticle (SPION)-labeled hESCs could migrate in the neural degenerated area and improve motor dysfunction in an AAV2-Htt171-82Q transfected Huntington rat model. Methods All animals were randomly allocated into three groups at first: HD group, sham group, and control group. After six weeks, the animals of the HD group and sham group were again divided into two subgroups depending on animals receiving either ipsilateral or contralateral hESCs transplantation. We performed cylinder test and stepping test every two weeks after AAV2-Htt171-82Q injection and hESCs transplantation. Stem cell tracking was performed once per two weeks using T2 and T2*-weighted images at 4.7 Tesla MRI. We also performed immunohistochemistry and immunofluorescence staining to detect the presence of hESCs markers, huntingtin protein aggregations, and iron in the striatum. Results After hESCs transplantation, the Htt virus-injected rats exhibited significant behavioral improvement in behavioral tests. SPION labeled hESCs showed migration with hypointense signal in MRI. The cells were positive with βIII-tubulin, GABA, and DARPP32. Conclusion Collectively, our results suggested that hESCs transplantation can be a potential treatment for motor dysfunction of Huntington's disease.Jaisan IslamKyoung Ha SoElina KCHyeong Cheol MoonAryun KimSang Hwan HyunSoochong KimYoung Seok ParkBMCarticleAdeno-associated virusHuntington's diseaseHuman embryonic stem cellNanoparticlesCell trackingMedicine (General)R5-920BiochemistryQD415-436ENStem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Adeno-associated virus Huntington's disease Human embryonic stem cell Nanoparticles Cell tracking Medicine (General) R5-920 Biochemistry QD415-436
spellingShingle	Adeno-associated virus Huntington's disease Human embryonic stem cell Nanoparticles Cell tracking Medicine (General) R5-920 Biochemistry QD415-436 Jaisan Islam Kyoung Ha So Elina KC Hyeong Cheol Moon Aryun Kim Sang Hwan Hyun Soochong Kim Young Seok Park Transplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington’s disease
description	Abstract Background Human embryonic stem cells (hESCs) transplantation had shown to provide a potential source of cells in neurodegenerative disease studies and lead to behavioral recovery in lentivirus transfected or, toxin-induced Huntington's disease (HD) rodent model. Here, we aimed to observe if transplantation of superparamagnetic iron oxide nanoparticle (SPION)-labeled hESCs could migrate in the neural degenerated area and improve motor dysfunction in an AAV2-Htt171-82Q transfected Huntington rat model. Methods All animals were randomly allocated into three groups at first: HD group, sham group, and control group. After six weeks, the animals of the HD group and sham group were again divided into two subgroups depending on animals receiving either ipsilateral or contralateral hESCs transplantation. We performed cylinder test and stepping test every two weeks after AAV2-Htt171-82Q injection and hESCs transplantation. Stem cell tracking was performed once per two weeks using T2 and T2*-weighted images at 4.7 Tesla MRI. We also performed immunohistochemistry and immunofluorescence staining to detect the presence of hESCs markers, huntingtin protein aggregations, and iron in the striatum. Results After hESCs transplantation, the Htt virus-injected rats exhibited significant behavioral improvement in behavioral tests. SPION labeled hESCs showed migration with hypointense signal in MRI. The cells were positive with βIII-tubulin, GABA, and DARPP32. Conclusion Collectively, our results suggested that hESCs transplantation can be a potential treatment for motor dysfunction of Huntington's disease.
format	article
author	Jaisan Islam Kyoung Ha So Elina KC Hyeong Cheol Moon Aryun Kim Sang Hwan Hyun Soochong Kim Young Seok Park
author_facet	Jaisan Islam Kyoung Ha So Elina KC Hyeong Cheol Moon Aryun Kim Sang Hwan Hyun Soochong Kim Young Seok Park
author_sort	Jaisan Islam
title	Transplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington’s disease
title_short	Transplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington’s disease
title_full	Transplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington’s disease
title_fullStr	Transplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington’s disease
title_full_unstemmed	Transplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington’s disease
title_sort	transplantation of human embryonic stem cells alleviates motor dysfunction in aav2-htt171-82q transfected rat model of huntington’s disease
publisher	BMC
publishDate	2021
url	https://doaj.org/article/ba7465a9291745fab8ea1a111bf002d5
work_keys_str_mv	AT jaisanislam transplantationofhumanembryonicstemcellsalleviatesmotordysfunctioninaav2htt17182qtransfectedratmodelofhuntingtonsdisease AT kyounghaso transplantationofhumanembryonicstemcellsalleviatesmotordysfunctioninaav2htt17182qtransfectedratmodelofhuntingtonsdisease AT elinakc transplantationofhumanembryonicstemcellsalleviatesmotordysfunctioninaav2htt17182qtransfectedratmodelofhuntingtonsdisease AT hyeongcheolmoon transplantationofhumanembryonicstemcellsalleviatesmotordysfunctioninaav2htt17182qtransfectedratmodelofhuntingtonsdisease AT aryunkim transplantationofhumanembryonicstemcellsalleviatesmotordysfunctioninaav2htt17182qtransfectedratmodelofhuntingtonsdisease AT sanghwanhyun transplantationofhumanembryonicstemcellsalleviatesmotordysfunctioninaav2htt17182qtransfectedratmodelofhuntingtonsdisease AT soochongkim transplantationofhumanembryonicstemcellsalleviatesmotordysfunctioninaav2htt17182qtransfectedratmodelofhuntingtonsdisease AT youngseokpark transplantationofhumanembryonicstemcellsalleviatesmotordysfunctioninaav2htt17182qtransfectedratmodelofhuntingtonsdisease
_version_	1718408221369040896

Transplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington’s disease

Ejemplares similares