An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples

Abstract Despite the excellent diagnostic applications of the current conventional immunoassay methods such as ELISA, immunostaining and Western blot for FAM134B detection, they are laborious, expensive and required a long turnaround time. Here, we report an electrochemical approach for rapid, sensi...

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Autores principales:	Farhadul Islam, Md Hakimul Haque, Sharda Yadav, Md Nazmul Islam, Vinod Gopalan, Nam-Trung Nguyen, Alfred K. Lam, Muhammad J. A. Shiddiky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba775d5fe7974ae7af6930adc805f1c7
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spelling	oai:doaj.org-article:ba775d5fe7974ae7af6930adc805f1c72021-12-02T11:52:42ZAn electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples10.1038/s41598-017-00206-82045-2322https://doaj.org/article/ba775d5fe7974ae7af6930adc805f1c72017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00206-8https://doaj.org/toc/2045-2322Abstract Despite the excellent diagnostic applications of the current conventional immunoassay methods such as ELISA, immunostaining and Western blot for FAM134B detection, they are laborious, expensive and required a long turnaround time. Here, we report an electrochemical approach for rapid, sensitive, and specific detection of FAM134B protein in biological (colon cancer cell extracts) and clinical (serum) samples. The approach utilises a differential pulse voltammetry (DPV) in the presence of the [Fe(CN)6]3−/4− redox system to quantify the FAM134B protein in a two-step strategy that involves (i) initial attachment of FAM134B antibody on the surface of extravidin-modified screen-printed carbon electrode, and (ii) subsequent detection of FAM134B protein present in the biological/clinical samples. The assay system was able to detect FAM134B protein at a concentration down to 10 pg μL−1 in phosphate buffered saline (pH 7.4) with a good inter-assay reproducibility (% RSD = <8.64, n = 3). We found excellent sensitivity and specificity for the analysis of FAM134B protein in a panel of colon cancer cell lines and serum samples. Finally, the assay was further validated with ELISA method. We believe that our assay could potentially lead a low-cost alternative to conventional immunological assays for target antigens analysis in point-of-care applications.Farhadul IslamMd Hakimul HaqueSharda YadavMd Nazmul IslamVinod GopalanNam-Trung NguyenAlfred K. LamMuhammad J. A. ShiddikyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Farhadul Islam Md Hakimul Haque Sharda Yadav Md Nazmul Islam Vinod Gopalan Nam-Trung Nguyen Alfred K. Lam Muhammad J. A. Shiddiky An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples
description	Abstract Despite the excellent diagnostic applications of the current conventional immunoassay methods such as ELISA, immunostaining and Western blot for FAM134B detection, they are laborious, expensive and required a long turnaround time. Here, we report an electrochemical approach for rapid, sensitive, and specific detection of FAM134B protein in biological (colon cancer cell extracts) and clinical (serum) samples. The approach utilises a differential pulse voltammetry (DPV) in the presence of the [Fe(CN)6]3−/4− redox system to quantify the FAM134B protein in a two-step strategy that involves (i) initial attachment of FAM134B antibody on the surface of extravidin-modified screen-printed carbon electrode, and (ii) subsequent detection of FAM134B protein present in the biological/clinical samples. The assay system was able to detect FAM134B protein at a concentration down to 10 pg μL−1 in phosphate buffered saline (pH 7.4) with a good inter-assay reproducibility (% RSD = <8.64, n = 3). We found excellent sensitivity and specificity for the analysis of FAM134B protein in a panel of colon cancer cell lines and serum samples. Finally, the assay was further validated with ELISA method. We believe that our assay could potentially lead a low-cost alternative to conventional immunological assays for target antigens analysis in point-of-care applications.
format	article
author	Farhadul Islam Md Hakimul Haque Sharda Yadav Md Nazmul Islam Vinod Gopalan Nam-Trung Nguyen Alfred K. Lam Muhammad J. A. Shiddiky
author_facet	Farhadul Islam Md Hakimul Haque Sharda Yadav Md Nazmul Islam Vinod Gopalan Nam-Trung Nguyen Alfred K. Lam Muhammad J. A. Shiddiky
author_sort	Farhadul Islam
title	An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples
title_short	An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples
title_full	An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples
title_fullStr	An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples
title_full_unstemmed	An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples
title_sort	electrochemical method for sensitive and rapid detection of fam134b protein in colon cancer samples
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ba775d5fe7974ae7af6930adc805f1c7
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An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples

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