‘Candidatus Cochliophilus cryoturris’ (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus

Abstract Free-living amoebae are well known for their role in controlling microbial community composition through grazing, but some groups, namely Acanthamoeba species, also frequently serve as hosts for bacterial symbionts. Here we report the first identification of a bacterial symbiont in the test...

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Autores principales:	Han-Fei Tsao, Ute Scheikl, Jean-Marie Volland, Martina Köhsler, Monika Bright, Julia Walochnik, Matthias Horn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba7b31b89a444e3cadb164e3cb58ff21
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spelling	oai:doaj.org-article:ba7b31b89a444e3cadb164e3cb58ff212021-12-02T12:32:24Z‘Candidatus Cochliophilus cryoturris’ (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus10.1038/s41598-017-03642-82045-2322https://doaj.org/article/ba7b31b89a444e3cadb164e3cb58ff212017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03642-8https://doaj.org/toc/2045-2322Abstract Free-living amoebae are well known for their role in controlling microbial community composition through grazing, but some groups, namely Acanthamoeba species, also frequently serve as hosts for bacterial symbionts. Here we report the first identification of a bacterial symbiont in the testate amoeba Cochliopodium. The amoeba was isolated from a cooling tower water sample and identified as C. minus. Fluorescence in situ hybridization and transmission electron microscopy revealed intracellular symbionts located in vacuoles. 16S rRNA-based phylogenetic analysis identified the endosymbiont as member of a monophyletic group within the family Coxiellaceae (Gammaprotebacteria; Legionellales), only moderately related to known amoeba symbionts. We propose to tentatively classify these bacteria as ‘Candidatus Cochliophilus cryoturris’. Our findings add both, a novel group of amoeba and a novel group of symbionts, to the growing list of bacteria-amoeba relationships.Han-Fei TsaoUte ScheiklJean-Marie VollandMartina KöhslerMonika BrightJulia WalochnikMatthias HornNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Han-Fei Tsao Ute Scheikl Jean-Marie Volland Martina Köhsler Monika Bright Julia Walochnik Matthias Horn ‘Candidatus Cochliophilus cryoturris’ (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus
description	Abstract Free-living amoebae are well known for their role in controlling microbial community composition through grazing, but some groups, namely Acanthamoeba species, also frequently serve as hosts for bacterial symbionts. Here we report the first identification of a bacterial symbiont in the testate amoeba Cochliopodium. The amoeba was isolated from a cooling tower water sample and identified as C. minus. Fluorescence in situ hybridization and transmission electron microscopy revealed intracellular symbionts located in vacuoles. 16S rRNA-based phylogenetic analysis identified the endosymbiont as member of a monophyletic group within the family Coxiellaceae (Gammaprotebacteria; Legionellales), only moderately related to known amoeba symbionts. We propose to tentatively classify these bacteria as ‘Candidatus Cochliophilus cryoturris’. Our findings add both, a novel group of amoeba and a novel group of symbionts, to the growing list of bacteria-amoeba relationships.
format	article
author	Han-Fei Tsao Ute Scheikl Jean-Marie Volland Martina Köhsler Monika Bright Julia Walochnik Matthias Horn
author_facet	Han-Fei Tsao Ute Scheikl Jean-Marie Volland Martina Köhsler Monika Bright Julia Walochnik Matthias Horn
author_sort	Han-Fei Tsao
title	‘Candidatus Cochliophilus cryoturris’ (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus
title_short	‘Candidatus Cochliophilus cryoturris’ (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus
title_full	‘Candidatus Cochliophilus cryoturris’ (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus
title_fullStr	‘Candidatus Cochliophilus cryoturris’ (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus
title_full_unstemmed	‘Candidatus Cochliophilus cryoturris’ (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus
title_sort	‘candidatus cochliophilus cryoturris’ (coxiellaceae), a symbiont of the testate amoeba cochliopodium minus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ba7b31b89a444e3cadb164e3cb58ff21
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‘Candidatus Cochliophilus cryoturris’ (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus

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