Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts

Abstract The liver is an important metabolic organ that controls homeostasis in the body. Moreover, it functions as a hematopoietic organ, while its metabolic function is low during development. Hepatocytes, which are parenchymal cells of the liver, acquire various metabolic functions by the maturat...

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Autores principales:	Kazuya Anzai, Kota Tsuruya, Kinuyo Ida, Tatehiro Kagawa, Yutaka Inagaki, Akihide Kamiya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba896f6abdb640a7b6c98832be244229
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spelling	oai:doaj.org-article:ba896f6abdb640a7b6c98832be2442292021-12-02T17:25:33ZKruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts10.1038/s41598-021-97937-62045-2322https://doaj.org/article/ba896f6abdb640a7b6c98832be2442292021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97937-6https://doaj.org/toc/2045-2322Abstract The liver is an important metabolic organ that controls homeostasis in the body. Moreover, it functions as a hematopoietic organ, while its metabolic function is low during development. Hepatocytes, which are parenchymal cells of the liver, acquire various metabolic functions by the maturation of hepatic progenitor cells during the fetal period; however, this molecular mechanism is still unclear. In this study, Kruppel-like factor 15 (KLF15) was identified as a new regulator of hepatic maturation through a comprehensive analysis of the expression of transcriptional regulators in mouse fetal and adult hepatocytes. KLF15 is a transcription factor whose expression in the liver increases from the embryonic stage throughout the developmental process. KLF15 induced the overexpression of liver function genes in mouse embryonic hepatocytes. Furthermore, we found that the expression of KLF15 could also induce the expression of liver function genes in hepatoblasts derived from human induced pluripotent stem cells (iPSCs). Moreover, KLF15 increased the promoter activity of tyrosine aminotransferase, a liver function gene. KLF15 also suppressed the proliferation of hepatoblasts. These results suggest that KLF15 induces hepatic maturation through the transcriptional activation of target genes and cell cycle control.Kazuya AnzaiKota TsuruyaKinuyo IdaTatehiro KagawaYutaka InagakiAkihide KamiyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazuya Anzai Kota Tsuruya Kinuyo Ida Tatehiro Kagawa Yutaka Inagaki Akihide Kamiya Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts
description	Abstract The liver is an important metabolic organ that controls homeostasis in the body. Moreover, it functions as a hematopoietic organ, while its metabolic function is low during development. Hepatocytes, which are parenchymal cells of the liver, acquire various metabolic functions by the maturation of hepatic progenitor cells during the fetal period; however, this molecular mechanism is still unclear. In this study, Kruppel-like factor 15 (KLF15) was identified as a new regulator of hepatic maturation through a comprehensive analysis of the expression of transcriptional regulators in mouse fetal and adult hepatocytes. KLF15 is a transcription factor whose expression in the liver increases from the embryonic stage throughout the developmental process. KLF15 induced the overexpression of liver function genes in mouse embryonic hepatocytes. Furthermore, we found that the expression of KLF15 could also induce the expression of liver function genes in hepatoblasts derived from human induced pluripotent stem cells (iPSCs). Moreover, KLF15 increased the promoter activity of tyrosine aminotransferase, a liver function gene. KLF15 also suppressed the proliferation of hepatoblasts. These results suggest that KLF15 induces hepatic maturation through the transcriptional activation of target genes and cell cycle control.
format	article
author	Kazuya Anzai Kota Tsuruya Kinuyo Ida Tatehiro Kagawa Yutaka Inagaki Akihide Kamiya
author_facet	Kazuya Anzai Kota Tsuruya Kinuyo Ida Tatehiro Kagawa Yutaka Inagaki Akihide Kamiya
author_sort	Kazuya Anzai
title	Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts
title_short	Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts
title_full	Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts
title_fullStr	Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts
title_full_unstemmed	Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts
title_sort	kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ba896f6abdb640a7b6c98832be244229
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Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts

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