Tips and tricks for successfully culturing and adapting human induced pluripotent stem cells

Reprogramming somatic cells toward pluripotency became possible over a decade ago. Since then, induced pluripotent stem cells (iPSCs) have served as a versatile and powerful tool not only for basic research but also with the long-term goal of using them in human cell transplantation after differenti...

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Autores principales:	Rocío Castro-Viñuelas, Clara Sanjurjo-Rodríguez, María Piñeiro-Ramil, Silvia Rodríguez-Fernández, Isidoro López-Baltar, Isaac Fuentes-Boquete, Francisco J. Blanco, Silvia Díaz-Prado
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	iPSCs feeders feeder-free culture troubleshooting tips and tricks Genetics QH426-470 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/ba8a07654c3c4c959e741f668ad3b654
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spelling	oai:doaj.org-article:ba8a07654c3c4c959e741f668ad3b6542021-12-02T05:01:47ZTips and tricks for successfully culturing and adapting human induced pluripotent stem cells2329-050110.1016/j.omtm.2021.10.013https://doaj.org/article/ba8a07654c3c4c959e741f668ad3b6542021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2329050121001704https://doaj.org/toc/2329-0501Reprogramming somatic cells toward pluripotency became possible over a decade ago. Since then, induced pluripotent stem cells (iPSCs) have served as a versatile and powerful tool not only for basic research but also with the long-term goal of using them in human cell transplantation after differentiation. Nonetheless, downstream applications are frequently blurred by the difficulties that researchers have to face when working with iPSCs, such as trouble with clonal selection, in vitro culture and cryopreservation, adaptation to feeder-free conditions, or expansion of the cells. Therefore, in this article we aim to provide other researchers with practical and detailed information to successfully culture and adapt iPSCs. Specifically, we (1) describe the most common problems when in-vitro culturing iPSCs onto feeder cells as well as its possible troubleshooting, and (2) compare different matrices and culture media for adapting the iPSCs to feeder-free conditions. We believe that the troubleshooting and recommendations provided in this article can be of use to other researchers working with iPSCs and who may be experiencing similar issues, hopefully enhancing the appeal of this promising cell source to be used for biomedical investigations, such as tissue engineering or regenerative medicine applications.Rocío Castro-ViñuelasClara Sanjurjo-RodríguezMaría Piñeiro-RamilSilvia Rodríguez-FernándezIsidoro López-BaltarIsaac Fuentes-BoqueteFrancisco J. BlancoSilvia Díaz-PradoElsevierarticleiPSCsfeedersfeeder-freeculturetroubleshootingtips and tricksGeneticsQH426-470CytologyQH573-671ENMolecular Therapy: Methods & Clinical Development, Vol 23, Iss , Pp 569-581 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	iPSCs feeders feeder-free culture troubleshooting tips and tricks Genetics QH426-470 Cytology QH573-671
spellingShingle	iPSCs feeders feeder-free culture troubleshooting tips and tricks Genetics QH426-470 Cytology QH573-671 Rocío Castro-Viñuelas Clara Sanjurjo-Rodríguez María Piñeiro-Ramil Silvia Rodríguez-Fernández Isidoro López-Baltar Isaac Fuentes-Boquete Francisco J. Blanco Silvia Díaz-Prado Tips and tricks for successfully culturing and adapting human induced pluripotent stem cells
description	Reprogramming somatic cells toward pluripotency became possible over a decade ago. Since then, induced pluripotent stem cells (iPSCs) have served as a versatile and powerful tool not only for basic research but also with the long-term goal of using them in human cell transplantation after differentiation. Nonetheless, downstream applications are frequently blurred by the difficulties that researchers have to face when working with iPSCs, such as trouble with clonal selection, in vitro culture and cryopreservation, adaptation to feeder-free conditions, or expansion of the cells. Therefore, in this article we aim to provide other researchers with practical and detailed information to successfully culture and adapt iPSCs. Specifically, we (1) describe the most common problems when in-vitro culturing iPSCs onto feeder cells as well as its possible troubleshooting, and (2) compare different matrices and culture media for adapting the iPSCs to feeder-free conditions. We believe that the troubleshooting and recommendations provided in this article can be of use to other researchers working with iPSCs and who may be experiencing similar issues, hopefully enhancing the appeal of this promising cell source to be used for biomedical investigations, such as tissue engineering or regenerative medicine applications.
format	article
author	Rocío Castro-Viñuelas Clara Sanjurjo-Rodríguez María Piñeiro-Ramil Silvia Rodríguez-Fernández Isidoro López-Baltar Isaac Fuentes-Boquete Francisco J. Blanco Silvia Díaz-Prado
author_facet	Rocío Castro-Viñuelas Clara Sanjurjo-Rodríguez María Piñeiro-Ramil Silvia Rodríguez-Fernández Isidoro López-Baltar Isaac Fuentes-Boquete Francisco J. Blanco Silvia Díaz-Prado
author_sort	Rocío Castro-Viñuelas
title	Tips and tricks for successfully culturing and adapting human induced pluripotent stem cells
title_short	Tips and tricks for successfully culturing and adapting human induced pluripotent stem cells
title_full	Tips and tricks for successfully culturing and adapting human induced pluripotent stem cells
title_fullStr	Tips and tricks for successfully culturing and adapting human induced pluripotent stem cells
title_full_unstemmed	Tips and tricks for successfully culturing and adapting human induced pluripotent stem cells
title_sort	tips and tricks for successfully culturing and adapting human induced pluripotent stem cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ba8a07654c3c4c959e741f668ad3b654
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Tips and tricks for successfully culturing and adapting human induced pluripotent stem cells

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