Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease

Abstract The immune response is critical in NAFLD pathogenesis, but the liver infiltrate’s composition and the role of each T cell population is still up for debate. To characterize liver pathogenesis in pediatric and adult cases, frequency and localization of immune cell populations [Cytotoxic T Ly...

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Autores principales:	Victoria Cairoli, Elena De Matteo, Daniela Rios, Carol Lezama, Marcela Galoppo, Paola Casciato, Eduardo Mullen, Cecilia Giadans, Gustavo Bertot, María Victoria Preciado, Pamela Valva
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba8e2ac2ddcc405f88c64e89a8aac39c
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spelling	oai:doaj.org-article:ba8e2ac2ddcc405f88c64e89a8aac39c2021-12-02T13:19:21ZHepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease10.1038/s41598-021-84674-z2045-2322https://doaj.org/article/ba8e2ac2ddcc405f88c64e89a8aac39c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84674-zhttps://doaj.org/toc/2045-2322Abstract The immune response is critical in NAFLD pathogenesis, but the liver infiltrate’s composition and the role of each T cell population is still up for debate. To characterize liver pathogenesis in pediatric and adult cases, frequency and localization of immune cell populations [Cytotoxic T Lymphocytes (CD8+), T helper Lymphocytes (CD4+), Regulatory T lymphocytes (Foxp3+) and Th17 (IL-17A+)] were evaluated. In portal/periportal (P/P) tracts, both age groups displayed a similar proportion of CD8+ and CD4+ lymphocytes. However, comparable Foxp3+ and IL-17A+ cell frequencies were observed in pediatric cases, meanwhile, in adults Foxp3+ was higher than IL-17A+ cells. Interestingly, IL-17A+ lymphocytes seemed to be nearly exclusive of P/P area in both age groups. In intralobular areas, both pediatric and adult cases showed CD8+ lymphocytes predominance with lower frequencies of CD4+ lymphocytes followed by Foxp3+ . Severe inflammation was associated with higher intralobular Foxp3+ lymphocytes (p = 0.026) in children, and lower P/P Foxp3+ and higher IL-17A+ lymphocytes in adults. All cases with fibrosis ≥ 2 displayed P/P low Foxp3+ and high IL-17A+ lymphocyte counts. Pediatric cases with worse steatosis showed high P/P CD4+ (p = 0.023) and intralobular CD8+ (p = 0.027) and CD4+ cells (p = 0.012). In NAFLD cases, the lymphocyte liver infiltrate composition differs between histological areas. Treg and Th17 balance seems to condition damage progression, denoting their important role in pathogenesis.Victoria CairoliElena De MatteoDaniela RiosCarol LezamaMarcela GaloppoPaola CasciatoEduardo MullenCecilia GiadansGustavo BertotMaría Victoria PreciadoPamela ValvaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Victoria Cairoli Elena De Matteo Daniela Rios Carol Lezama Marcela Galoppo Paola Casciato Eduardo Mullen Cecilia Giadans Gustavo Bertot María Victoria Preciado Pamela Valva Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
description	Abstract The immune response is critical in NAFLD pathogenesis, but the liver infiltrate’s composition and the role of each T cell population is still up for debate. To characterize liver pathogenesis in pediatric and adult cases, frequency and localization of immune cell populations [Cytotoxic T Lymphocytes (CD8+), T helper Lymphocytes (CD4+), Regulatory T lymphocytes (Foxp3+) and Th17 (IL-17A+)] were evaluated. In portal/periportal (P/P) tracts, both age groups displayed a similar proportion of CD8+ and CD4+ lymphocytes. However, comparable Foxp3+ and IL-17A+ cell frequencies were observed in pediatric cases, meanwhile, in adults Foxp3+ was higher than IL-17A+ cells. Interestingly, IL-17A+ lymphocytes seemed to be nearly exclusive of P/P area in both age groups. In intralobular areas, both pediatric and adult cases showed CD8+ lymphocytes predominance with lower frequencies of CD4+ lymphocytes followed by Foxp3+ . Severe inflammation was associated with higher intralobular Foxp3+ lymphocytes (p = 0.026) in children, and lower P/P Foxp3+ and higher IL-17A+ lymphocytes in adults. All cases with fibrosis ≥ 2 displayed P/P low Foxp3+ and high IL-17A+ lymphocyte counts. Pediatric cases with worse steatosis showed high P/P CD4+ (p = 0.023) and intralobular CD8+ (p = 0.027) and CD4+ cells (p = 0.012). In NAFLD cases, the lymphocyte liver infiltrate composition differs between histological areas. Treg and Th17 balance seems to condition damage progression, denoting their important role in pathogenesis.
format	article
author	Victoria Cairoli Elena De Matteo Daniela Rios Carol Lezama Marcela Galoppo Paola Casciato Eduardo Mullen Cecilia Giadans Gustavo Bertot María Victoria Preciado Pamela Valva
author_facet	Victoria Cairoli Elena De Matteo Daniela Rios Carol Lezama Marcela Galoppo Paola Casciato Eduardo Mullen Cecilia Giadans Gustavo Bertot María Victoria Preciado Pamela Valva
author_sort	Victoria Cairoli
title	Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
title_short	Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
title_full	Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
title_fullStr	Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
title_full_unstemmed	Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
title_sort	hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ba8e2ac2ddcc405f88c64e89a8aac39c
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Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease

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