The immune cell landscape of peripheral blood mononuclear cells from PNS patients

Abstract Existing research suggests that the human immune system and immune cells are involved in the pathogenesis of nephrotic syndrome, but there is still a lack of direct evidence. This study tried to analyze the profiling of immune cells in the peripheral blood of steroid-sensitive nephrotic syn...

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Autores principales:	Qing Ye, Chao Zhou, Sisi Li, Jingjing Wang, Fei Liu, Zhixia Liu, Jianhua Mao, Haidong Fu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba9135210fa74a3ca5d1ed3e57b84727
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spelling	oai:doaj.org-article:ba9135210fa74a3ca5d1ed3e57b847272021-12-02T17:44:55ZThe immune cell landscape of peripheral blood mononuclear cells from PNS patients10.1038/s41598-021-92573-62045-2322https://doaj.org/article/ba9135210fa74a3ca5d1ed3e57b847272021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92573-6https://doaj.org/toc/2045-2322Abstract Existing research suggests that the human immune system and immune cells are involved in the pathogenesis of nephrotic syndrome, but there is still a lack of direct evidence. This study tried to analyze the profiling of immune cells in the peripheral blood of steroid-sensitive nephrotic syndrome (SSNS) patients and steroid-resistant nephrotic syndrome (SRNS) patients before and after standard steroid treatment to clarify the immunological mechanism of nephrotic syndrome patients. The number and proportion of CD4 + T cells in patients with nephrotic syndrome remained unchanged. However, there is an imbalance of Th1 and Th2 and an excessive increase of Th17 cells. The number of CD8 + T cells and the number of effector CD8 + T cells in them increased significantly, but only in SSNS, the number of activated CD8 + T cells increased, and the number of activated Treg cells decreased significantly. Nephrotic syndrome patients also have B cell disorder, and it is more prominent in SSNS patients. Compared with the normal control, only the number of B cells and plasmablast in SSNS patients increased significantly (Z = − 2.20, P = 0.028). This study also observed that transitional B cells decreased in both SSNS and SRNS patients, but SSNS patients' decrease was lower than in SRNS patients. Compared with normal controls, monocytes in patients with nephrotic syndrome decreased significantly. The main reason was that Non-classical Monocyte decreased, while Classical Monocyte increased slightly. The total number of NK cells did not change, but the internal cell subgroups' composition occurred. Changes, realized as CD56hi NK cells increased, CD56low NK cells decreased; and the above trend is more evident in SSNS patients. Patients with nephrotic syndrome have immune disorders, including T cells, B cells, Monocytes, and NK cells. It can be confirmed that immune factors are involved in the pathogenesis of the nephrotic syndrome.Qing YeChao ZhouSisi LiJingjing WangFei LiuZhixia LiuJianhua MaoHaidong FuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qing Ye Chao Zhou Sisi Li Jingjing Wang Fei Liu Zhixia Liu Jianhua Mao Haidong Fu The immune cell landscape of peripheral blood mononuclear cells from PNS patients
description	Abstract Existing research suggests that the human immune system and immune cells are involved in the pathogenesis of nephrotic syndrome, but there is still a lack of direct evidence. This study tried to analyze the profiling of immune cells in the peripheral blood of steroid-sensitive nephrotic syndrome (SSNS) patients and steroid-resistant nephrotic syndrome (SRNS) patients before and after standard steroid treatment to clarify the immunological mechanism of nephrotic syndrome patients. The number and proportion of CD4 + T cells in patients with nephrotic syndrome remained unchanged. However, there is an imbalance of Th1 and Th2 and an excessive increase of Th17 cells. The number of CD8 + T cells and the number of effector CD8 + T cells in them increased significantly, but only in SSNS, the number of activated CD8 + T cells increased, and the number of activated Treg cells decreased significantly. Nephrotic syndrome patients also have B cell disorder, and it is more prominent in SSNS patients. Compared with the normal control, only the number of B cells and plasmablast in SSNS patients increased significantly (Z = − 2.20, P = 0.028). This study also observed that transitional B cells decreased in both SSNS and SRNS patients, but SSNS patients' decrease was lower than in SRNS patients. Compared with normal controls, monocytes in patients with nephrotic syndrome decreased significantly. The main reason was that Non-classical Monocyte decreased, while Classical Monocyte increased slightly. The total number of NK cells did not change, but the internal cell subgroups' composition occurred. Changes, realized as CD56hi NK cells increased, CD56low NK cells decreased; and the above trend is more evident in SSNS patients. Patients with nephrotic syndrome have immune disorders, including T cells, B cells, Monocytes, and NK cells. It can be confirmed that immune factors are involved in the pathogenesis of the nephrotic syndrome.
format	article
author	Qing Ye Chao Zhou Sisi Li Jingjing Wang Fei Liu Zhixia Liu Jianhua Mao Haidong Fu
author_facet	Qing Ye Chao Zhou Sisi Li Jingjing Wang Fei Liu Zhixia Liu Jianhua Mao Haidong Fu
author_sort	Qing Ye
title	The immune cell landscape of peripheral blood mononuclear cells from PNS patients
title_short	The immune cell landscape of peripheral blood mononuclear cells from PNS patients
title_full	The immune cell landscape of peripheral blood mononuclear cells from PNS patients
title_fullStr	The immune cell landscape of peripheral blood mononuclear cells from PNS patients
title_full_unstemmed	The immune cell landscape of peripheral blood mononuclear cells from PNS patients
title_sort	immune cell landscape of peripheral blood mononuclear cells from pns patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ba9135210fa74a3ca5d1ed3e57b84727
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The immune cell landscape of peripheral blood mononuclear cells from PNS patients

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