Transcriptional Differences Guided Discovery and Genetic Identification of Coprogen and Dimerumic Acid Siderophores in Metarhizium robertsii

Siderophores are small molecular iron chelators and participate in the multiple cellular processes in fungi. In this study, biosynthesis gene clusters of coprogens and dimerumic acids were identified by transcriptional level differences of genes related to iron deficiency conditions in Metarhizium r...

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Autores principales:	Jinyu Zhang, Peng Zhang, Guohong Zeng, Guangwei Wu, Landa Qi, Guocan Chen, Weiguo Fang, Wen-Bing Yin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	siderophore coprogen dimerumic acid fungi biosynthesis NRPS Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ba933fc1702b4cdc9860a9185ac62341
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spelling	oai:doaj.org-article:ba933fc1702b4cdc9860a9185ac623412021-12-01T01:46:32ZTranscriptional Differences Guided Discovery and Genetic Identification of Coprogen and Dimerumic Acid Siderophores in Metarhizium robertsii1664-302X10.3389/fmicb.2021.783609https://doaj.org/article/ba933fc1702b4cdc9860a9185ac623412021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.783609/fullhttps://doaj.org/toc/1664-302XSiderophores are small molecular iron chelators and participate in the multiple cellular processes in fungi. In this study, biosynthesis gene clusters of coprogens and dimerumic acids were identified by transcriptional level differences of genes related to iron deficiency conditions in Metarhizium robertsii. This leads to the characterization of new coprogen metachelin C (1) and five known siderophores metachelin A (2), metachelin A-CE (3), metachelin B (4), dimerumic acid 11-mannoside (5), and dimerumic acid (6). The structure of metachelin C (1) was elucidated by NMR spectroscopy and HR-ESI-MS analysis. Genetic deletions of mrsidA, and mrsidD abolished the production of compounds 1–6 that implied their involvement in the biosynthesis of coprogen and dimerumic acid. Interestingly, NRPS gene mrsidD is responsible for biosynthesis of both coprogen and dimerumic acid, thus we proposed plausible biosynthetic pathways for the synthesis of coprogen and dimerumic acid siderophores. Therefore, our study provides the genetic basis for understanding the biosynthetic pathway of coprogen and dimerumic acid in Metarhizium robertsii.Jinyu ZhangJinyu ZhangPeng ZhangGuohong ZengGuangwei WuLanda QiGuocan ChenWeiguo FangWen-Bing YinWen-Bing YinFrontiers Media S.A.articlesiderophorecoprogendimerumic acidfungibiosynthesisNRPSMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	siderophore coprogen dimerumic acid fungi biosynthesis NRPS Microbiology QR1-502
spellingShingle	siderophore coprogen dimerumic acid fungi biosynthesis NRPS Microbiology QR1-502 Jinyu Zhang Jinyu Zhang Peng Zhang Guohong Zeng Guangwei Wu Landa Qi Guocan Chen Weiguo Fang Wen-Bing Yin Wen-Bing Yin Transcriptional Differences Guided Discovery and Genetic Identification of Coprogen and Dimerumic Acid Siderophores in Metarhizium robertsii
description	Siderophores are small molecular iron chelators and participate in the multiple cellular processes in fungi. In this study, biosynthesis gene clusters of coprogens and dimerumic acids were identified by transcriptional level differences of genes related to iron deficiency conditions in Metarhizium robertsii. This leads to the characterization of new coprogen metachelin C (1) and five known siderophores metachelin A (2), metachelin A-CE (3), metachelin B (4), dimerumic acid 11-mannoside (5), and dimerumic acid (6). The structure of metachelin C (1) was elucidated by NMR spectroscopy and HR-ESI-MS analysis. Genetic deletions of mrsidA, and mrsidD abolished the production of compounds 1–6 that implied their involvement in the biosynthesis of coprogen and dimerumic acid. Interestingly, NRPS gene mrsidD is responsible for biosynthesis of both coprogen and dimerumic acid, thus we proposed plausible biosynthetic pathways for the synthesis of coprogen and dimerumic acid siderophores. Therefore, our study provides the genetic basis for understanding the biosynthetic pathway of coprogen and dimerumic acid in Metarhizium robertsii.
format	article
author	Jinyu Zhang Jinyu Zhang Peng Zhang Guohong Zeng Guangwei Wu Landa Qi Guocan Chen Weiguo Fang Wen-Bing Yin Wen-Bing Yin
author_facet	Jinyu Zhang Jinyu Zhang Peng Zhang Guohong Zeng Guangwei Wu Landa Qi Guocan Chen Weiguo Fang Wen-Bing Yin Wen-Bing Yin
author_sort	Jinyu Zhang
title	Transcriptional Differences Guided Discovery and Genetic Identification of Coprogen and Dimerumic Acid Siderophores in Metarhizium robertsii
title_short	Transcriptional Differences Guided Discovery and Genetic Identification of Coprogen and Dimerumic Acid Siderophores in Metarhizium robertsii
title_full	Transcriptional Differences Guided Discovery and Genetic Identification of Coprogen and Dimerumic Acid Siderophores in Metarhizium robertsii
title_fullStr	Transcriptional Differences Guided Discovery and Genetic Identification of Coprogen and Dimerumic Acid Siderophores in Metarhizium robertsii
title_full_unstemmed	Transcriptional Differences Guided Discovery and Genetic Identification of Coprogen and Dimerumic Acid Siderophores in Metarhizium robertsii
title_sort	transcriptional differences guided discovery and genetic identification of coprogen and dimerumic acid siderophores in metarhizium robertsii
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ba933fc1702b4cdc9860a9185ac62341
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Transcriptional Differences Guided Discovery and Genetic Identification of Coprogen and Dimerumic Acid Siderophores in Metarhizium robertsii

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