Vertical transmission of zika virus in Aedes albopictus.

<h4>Background</h4>Zika virus (ZIKV) is an arthropod-borne flavivirus transmitted by Aedes mosquitoes. Aedes albopictus is an important vector of ZIKV worldwide. To date, most experiments have focused on the vertical transmission of ZIKV in Ae. aegypti, while studies on Ae. albopictus ar...

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Autores principales:	Zetian Lai, Tengfei Zhou, Jiayong Zhou, Shuang Liu, Ye Xu, Jinbao Gu, Guiyun Yan, Xiao-Guang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2020
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/ba982e9b78074c70bb56767b7fa24732
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spelling	oai:doaj.org-article:ba982e9b78074c70bb56767b7fa247322021-12-02T20:23:25ZVertical transmission of zika virus in Aedes albopictus.1935-27271935-273510.1371/journal.pntd.0008776https://doaj.org/article/ba982e9b78074c70bb56767b7fa247322020-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0008776https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Zika virus (ZIKV) is an arthropod-borne flavivirus transmitted by Aedes mosquitoes. Aedes albopictus is an important vector of ZIKV worldwide. To date, most experiments have focused on the vertical transmission of ZIKV in Ae. aegypti, while studies on Ae. albopictus are very limited. To explore vertical transmission in Ae. albopictus, a series of laboratory studies were carried out.<h4>Methodology/principal findings</h4>In this study, Ae. albopictus were blood-fed with ZIKV-infectious blood, and the ovaries and offspring viral infection rates were analyzed by reverse transcription PCR (RT-PCR), real-time reverse transcription PCR (RT-qPCR) and immunohistochemistry (IHC). ZIKV was detected in the ovaries and oviposited eggs in two gonotrophic cycles. The minimum filial egg infection rates in two gonotrophic cycles were 2.06% and 0.69%, and the effective population transmission rate was 1.87%. The hatching, pupation, and emergence rates of infected offspring were not significantly different from those of uninfected offspring, indicating that ZIKV did not prevent the offspring from completing the growth and development process. ZIKV was detected in three of thirteen C57BL/6 suckling mice bitten by ZIKV-positive F1 females, and the viremia persisted for at least seven days.<h4>Conclusions/significance</h4>ZIKV can be vertically transmitted in Ae. albopictus via transovarial transmission. The vertical transmission rates in F1 eggs and adults were 2.06% and 1.87%, respectively. Even though the vertical transmission rates were low, the female mosquitoes infected via the congenital route horizontally transmitted ZIKV to suckling mice through bloodsucking. This is the first experimental evidence of offspring with vertically transmitted ZIKV initiating new horizontal transmission. The present study deepens the understanding of the vertical transmission of flaviviruses in Aedes mosquitoes and sheds light on the prevention and control of mosquito-borne diseases.Zetian LaiTengfei ZhouJiayong ZhouShuang LiuYe XuJinbao GuGuiyun YanXiao-Guang ChenPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008776 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Zetian Lai Tengfei Zhou Jiayong Zhou Shuang Liu Ye Xu Jinbao Gu Guiyun Yan Xiao-Guang Chen Vertical transmission of zika virus in Aedes albopictus.
description	<h4>Background</h4>Zika virus (ZIKV) is an arthropod-borne flavivirus transmitted by Aedes mosquitoes. Aedes albopictus is an important vector of ZIKV worldwide. To date, most experiments have focused on the vertical transmission of ZIKV in Ae. aegypti, while studies on Ae. albopictus are very limited. To explore vertical transmission in Ae. albopictus, a series of laboratory studies were carried out.<h4>Methodology/principal findings</h4>In this study, Ae. albopictus were blood-fed with ZIKV-infectious blood, and the ovaries and offspring viral infection rates were analyzed by reverse transcription PCR (RT-PCR), real-time reverse transcription PCR (RT-qPCR) and immunohistochemistry (IHC). ZIKV was detected in the ovaries and oviposited eggs in two gonotrophic cycles. The minimum filial egg infection rates in two gonotrophic cycles were 2.06% and 0.69%, and the effective population transmission rate was 1.87%. The hatching, pupation, and emergence rates of infected offspring were not significantly different from those of uninfected offspring, indicating that ZIKV did not prevent the offspring from completing the growth and development process. ZIKV was detected in three of thirteen C57BL/6 suckling mice bitten by ZIKV-positive F1 females, and the viremia persisted for at least seven days.<h4>Conclusions/significance</h4>ZIKV can be vertically transmitted in Ae. albopictus via transovarial transmission. The vertical transmission rates in F1 eggs and adults were 2.06% and 1.87%, respectively. Even though the vertical transmission rates were low, the female mosquitoes infected via the congenital route horizontally transmitted ZIKV to suckling mice through bloodsucking. This is the first experimental evidence of offspring with vertically transmitted ZIKV initiating new horizontal transmission. The present study deepens the understanding of the vertical transmission of flaviviruses in Aedes mosquitoes and sheds light on the prevention and control of mosquito-borne diseases.
format	article
author	Zetian Lai Tengfei Zhou Jiayong Zhou Shuang Liu Ye Xu Jinbao Gu Guiyun Yan Xiao-Guang Chen
author_facet	Zetian Lai Tengfei Zhou Jiayong Zhou Shuang Liu Ye Xu Jinbao Gu Guiyun Yan Xiao-Guang Chen
author_sort	Zetian Lai
title	Vertical transmission of zika virus in Aedes albopictus.
title_short	Vertical transmission of zika virus in Aedes albopictus.
title_full	Vertical transmission of zika virus in Aedes albopictus.
title_fullStr	Vertical transmission of zika virus in Aedes albopictus.
title_full_unstemmed	Vertical transmission of zika virus in Aedes albopictus.
title_sort	vertical transmission of zika virus in aedes albopictus.
publisher	Public Library of Science (PLoS)
publishDate	2020
url	https://doaj.org/article/ba982e9b78074c70bb56767b7fa24732
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Vertical transmission of zika virus in Aedes albopictus.

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