Single vector non-leaky gene expression system for Drosophila melanogaster

Abstract An ideal transgenic gene expression system is inducible, non-leaky, and well tolerated by the target organism. While the former has been satisfactorily realized, leakiness and heavy physiological burden imposed by the existing systems are still prominent hurdles in their successful implemen...

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Autores principales:	Arslan Akmammedov, Marco Geigges, Renato Paro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba9be278943c409c82f35c50190654d6
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spelling	oai:doaj.org-article:ba9be278943c409c82f35c50190654d62021-12-02T16:08:11ZSingle vector non-leaky gene expression system for Drosophila melanogaster10.1038/s41598-017-07282-w2045-2322https://doaj.org/article/ba9be278943c409c82f35c50190654d62017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07282-whttps://doaj.org/toc/2045-2322Abstract An ideal transgenic gene expression system is inducible, non-leaky, and well tolerated by the target organism. While the former has been satisfactorily realized, leakiness and heavy physiological burden imposed by the existing systems are still prominent hurdles in their successful implementation. Here we describe a new system for non-leaky expression of transgenes in Drosophila. PRExpress is based on a single transgenic construct built from endogenous components, the inducible hsp70 promoter and a multimerized copy of a Polycomb response element (PRE) controlled by epigenetic chromatin regulators of the Polycomb group. We show that this system is non-leaky, rapidly and strongly inducible, and reversible. To make the application of PRExpress user-friendly, we deliver the construct via site-specific integration.Arslan AkmammedovMarco GeiggesRenato ParoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arslan Akmammedov Marco Geigges Renato Paro Single vector non-leaky gene expression system for Drosophila melanogaster
description	Abstract An ideal transgenic gene expression system is inducible, non-leaky, and well tolerated by the target organism. While the former has been satisfactorily realized, leakiness and heavy physiological burden imposed by the existing systems are still prominent hurdles in their successful implementation. Here we describe a new system for non-leaky expression of transgenes in Drosophila. PRExpress is based on a single transgenic construct built from endogenous components, the inducible hsp70 promoter and a multimerized copy of a Polycomb response element (PRE) controlled by epigenetic chromatin regulators of the Polycomb group. We show that this system is non-leaky, rapidly and strongly inducible, and reversible. To make the application of PRExpress user-friendly, we deliver the construct via site-specific integration.
format	article
author	Arslan Akmammedov Marco Geigges Renato Paro
author_facet	Arslan Akmammedov Marco Geigges Renato Paro
author_sort	Arslan Akmammedov
title	Single vector non-leaky gene expression system for Drosophila melanogaster
title_short	Single vector non-leaky gene expression system for Drosophila melanogaster
title_full	Single vector non-leaky gene expression system for Drosophila melanogaster
title_fullStr	Single vector non-leaky gene expression system for Drosophila melanogaster
title_full_unstemmed	Single vector non-leaky gene expression system for Drosophila melanogaster
title_sort	single vector non-leaky gene expression system for drosophila melanogaster
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ba9be278943c409c82f35c50190654d6
work_keys_str_mv	AT arslanakmammedov singlevectornonleakygeneexpressionsystemfordrosophilamelanogaster AT marcogeigges singlevectornonleakygeneexpressionsystemfordrosophilamelanogaster AT renatoparo singlevectornonleakygeneexpressionsystemfordrosophilamelanogaster
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Single vector non-leaky gene expression system for Drosophila melanogaster

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