miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta

Abstract Aedes aegypti L. is the most important vector of arboviruses such as dengue, Zika, chikungunya, Mayaro, and yellow fever, which impact millions of people’s health per year. MicroRNA profile has been described in some mosquito species as being important for biological processes such as diges...

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Autores principales:	Iram Pablo Rodríguez-Sanchez, Daniel Rafael Saldaña-Torres, Olga Karina Villanueva-Segura, Maria Lourdes Garza-Rodriguez, Mayra A. Gómez-Govea, Ghongwei Liang, María de Lourdes Ramírez-Ahuja, Margarita De La Luz Martinez-Fierro, Ivan Delgado-Enciso, Laura E. Martinez-de-Villarreal, Yu Zhou, Adriana E. Flores-Suarez, Xi Chen, Diana Resendez-Pérez, Chen-Yu Zhang, Gustavo Ponce-Garcia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/baa54f0576fd428789b87f9c6af8d959
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spelling	oai:doaj.org-article:baa54f0576fd428789b87f9c6af8d9592021-12-02T16:51:31ZmiRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta10.1038/s41598-021-90095-92045-2322https://doaj.org/article/baa54f0576fd428789b87f9c6af8d9592021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90095-9https://doaj.org/toc/2045-2322Abstract Aedes aegypti L. is the most important vector of arboviruses such as dengue, Zika, chikungunya, Mayaro, and yellow fever, which impact millions of people’s health per year. MicroRNA profile has been described in some mosquito species as being important for biological processes such as digestion of blood, oviposition, sexual differentiation, insecticide resistance, and pathogens dissemination. We identified the miRNAs of Ae. aegypti females, males and eggs of a reference insecticide susceptible strain New Orleans and compared them with those other insects to determine miRNA fingerprint by new-generation sequencing. The sequences were analyzed using data mining tools and categorization, followed by differential expression analysis and conservation with other insects. A total of 55 conserved miRNAs were identified, of which 34 were of holometabolous insects and 21 shared with hemimetabolous insects. Of these miRNAs, 32 had differential expression within the stages analyzed. Three predominant functions of miRNA were related to embryonic development regulation, metamorphosis, and basal functions. The findings of this research describe new information on Ae. aegypti physiology which could be useful for the development of new control strategies, particularly in mosquito development and metamorphosis processes.Iram Pablo Rodríguez-SanchezDaniel Rafael Saldaña-TorresOlga Karina Villanueva-SeguraMaria Lourdes Garza-RodriguezMayra A. Gómez-GoveaGhongwei LiangMaría de Lourdes Ramírez-AhujaMargarita De La Luz Martinez-FierroIvan Delgado-EncisoLaura E. Martinez-de-VillarrealYu ZhouAdriana E. Flores-SuarezXi ChenDiana Resendez-PérezChen-Yu ZhangGustavo Ponce-GarciaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Iram Pablo Rodríguez-Sanchez Daniel Rafael Saldaña-Torres Olga Karina Villanueva-Segura Maria Lourdes Garza-Rodriguez Mayra A. Gómez-Govea Ghongwei Liang María de Lourdes Ramírez-Ahuja Margarita De La Luz Martinez-Fierro Ivan Delgado-Enciso Laura E. Martinez-de-Villarreal Yu Zhou Adriana E. Flores-Suarez Xi Chen Diana Resendez-Pérez Chen-Yu Zhang Gustavo Ponce-Garcia miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta
description	Abstract Aedes aegypti L. is the most important vector of arboviruses such as dengue, Zika, chikungunya, Mayaro, and yellow fever, which impact millions of people’s health per year. MicroRNA profile has been described in some mosquito species as being important for biological processes such as digestion of blood, oviposition, sexual differentiation, insecticide resistance, and pathogens dissemination. We identified the miRNAs of Ae. aegypti females, males and eggs of a reference insecticide susceptible strain New Orleans and compared them with those other insects to determine miRNA fingerprint by new-generation sequencing. The sequences were analyzed using data mining tools and categorization, followed by differential expression analysis and conservation with other insects. A total of 55 conserved miRNAs were identified, of which 34 were of holometabolous insects and 21 shared with hemimetabolous insects. Of these miRNAs, 32 had differential expression within the stages analyzed. Three predominant functions of miRNA were related to embryonic development regulation, metamorphosis, and basal functions. The findings of this research describe new information on Ae. aegypti physiology which could be useful for the development of new control strategies, particularly in mosquito development and metamorphosis processes.
format	article
author	Iram Pablo Rodríguez-Sanchez Daniel Rafael Saldaña-Torres Olga Karina Villanueva-Segura Maria Lourdes Garza-Rodriguez Mayra A. Gómez-Govea Ghongwei Liang María de Lourdes Ramírez-Ahuja Margarita De La Luz Martinez-Fierro Ivan Delgado-Enciso Laura E. Martinez-de-Villarreal Yu Zhou Adriana E. Flores-Suarez Xi Chen Diana Resendez-Pérez Chen-Yu Zhang Gustavo Ponce-Garcia
author_facet	Iram Pablo Rodríguez-Sanchez Daniel Rafael Saldaña-Torres Olga Karina Villanueva-Segura Maria Lourdes Garza-Rodriguez Mayra A. Gómez-Govea Ghongwei Liang María de Lourdes Ramírez-Ahuja Margarita De La Luz Martinez-Fierro Ivan Delgado-Enciso Laura E. Martinez-de-Villarreal Yu Zhou Adriana E. Flores-Suarez Xi Chen Diana Resendez-Pérez Chen-Yu Zhang Gustavo Ponce-Garcia
author_sort	Iram Pablo Rodríguez-Sanchez
title	miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta
title_short	miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta
title_full	miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta
title_fullStr	miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta
title_full_unstemmed	miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta
title_sort	mirnas of aedes aegypti (linnaeus 1762) conserved in six orders of the class insecta
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/baa54f0576fd428789b87f9c6af8d959
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miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta

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