An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification

In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated...

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Autores principales:	Hongmin Cai, Hebang Yao, Tingting Li, Cedric A. J. Hutter, Yanfang Li, Yannan Tang, Markus A. Seeger, Dianfan Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/baab4a34e2bb410bbf23d2caba607e37
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spelling	oai:doaj.org-article:baab4a34e2bb410bbf23d2caba607e372021-12-02T12:33:13ZAn improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification10.1038/s42003-020-01478-z2399-3642https://doaj.org/article/baab4a34e2bb410bbf23d2caba607e372020-12-01T00:00:00Zhttps://doi.org/10.1038/s42003-020-01478-zhttps://doaj.org/toc/2399-3642In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated synthetic nanobodies against TGP to facilitate purification.Hongmin CaiHebang YaoTingting LiCedric A. J. HutterYanfang LiYannan TangMarkus A. SeegerDianfan LiNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 3, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Hongmin Cai Hebang Yao Tingting Li Cedric A. J. Hutter Yanfang Li Yannan Tang Markus A. Seeger Dianfan Li An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
description	In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated synthetic nanobodies against TGP to facilitate purification.
format	article
author	Hongmin Cai Hebang Yao Tingting Li Cedric A. J. Hutter Yanfang Li Yannan Tang Markus A. Seeger Dianfan Li
author_facet	Hongmin Cai Hebang Yao Tingting Li Cedric A. J. Hutter Yanfang Li Yannan Tang Markus A. Seeger Dianfan Li
author_sort	Hongmin Cai
title	An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
title_short	An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
title_full	An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
title_fullStr	An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
title_full_unstemmed	An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
title_sort	improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/baab4a34e2bb410bbf23d2caba607e37
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An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification

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