An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | article |
| Language: | EN |
| Published: |
Nature Portfolio
2020
|
| Subjects: | |
| Online Access: | https://doaj.org/article/baab4a34e2bb410bbf23d2caba607e37 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|