Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes

Abstract In contrast to amniotes (reptiles, birds and mammals), anamniotes (fishes and amphibians) can effectively regenerate body appendages such as fins, limbs and tails. Why such a useful capability was progressively lost in amniotes remains unknown. As we have hypothesized recently, one of the r...

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Autores principales:	Anastasiya S. Ivanova, Daria D. Korotkova, Galina V. Ermakova, Natalia Yu. Martynova, Andrey G. Zaraisky, Maria B. Tereshina
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/baab8ae0beba4a9c944d2371d79d12a6
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spelling	oai:doaj.org-article:baab8ae0beba4a9c944d2371d79d12a62021-12-02T15:08:17ZRas-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes10.1038/s41598-018-30811-02045-2322https://doaj.org/article/baab8ae0beba4a9c944d2371d79d12a62018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30811-0https://doaj.org/toc/2045-2322Abstract In contrast to amniotes (reptiles, birds and mammals), anamniotes (fishes and amphibians) can effectively regenerate body appendages such as fins, limbs and tails. Why such a useful capability was progressively lost in amniotes remains unknown. As we have hypothesized recently, one of the reasons for this could be loss of some genes regulating the regeneration in evolution of amniotes. Here, we demonstrate the validity of this hypothesis by showing that genes of small GTPases Ras-dva1 and Ras-dva2, that had been lost in a stepwise manner during evolution of amniotes and disappeared completely in placental mammals, are important for regeneration in anamniotes. Both Ras-dva genes are quickly activated in regenerative wound epithelium and blastema forming in the amputated adult Danio rerio fins and Xenopus laevis tadpoles’ tails and hindlimb buds. Down-regulation of any of two Ras-dva genes in fish and frog resulted in a retardation of regeneration accompanied by down-regulation of the regeneration marker genes. On the other hand, Ras-dva over-expression in tadpoles’ tails restores regeneration capacity during the refractory period when regeneration is blocked due to natural reasons. Thus our data on Ras-dva genes, which were eliminated in amniotes but play role in anamniotes regeneration regulation, satisfy our hypothesis.Anastasiya S. IvanovaDaria D. KorotkovaGalina V. ErmakovaNatalia Yu. MartynovaAndrey G. ZaraiskyMaria B. TereshinaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anastasiya S. Ivanova Daria D. Korotkova Galina V. Ermakova Natalia Yu. Martynova Andrey G. Zaraisky Maria B. Tereshina Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes
description	Abstract In contrast to amniotes (reptiles, birds and mammals), anamniotes (fishes and amphibians) can effectively regenerate body appendages such as fins, limbs and tails. Why such a useful capability was progressively lost in amniotes remains unknown. As we have hypothesized recently, one of the reasons for this could be loss of some genes regulating the regeneration in evolution of amniotes. Here, we demonstrate the validity of this hypothesis by showing that genes of small GTPases Ras-dva1 and Ras-dva2, that had been lost in a stepwise manner during evolution of amniotes and disappeared completely in placental mammals, are important for regeneration in anamniotes. Both Ras-dva genes are quickly activated in regenerative wound epithelium and blastema forming in the amputated adult Danio rerio fins and Xenopus laevis tadpoles’ tails and hindlimb buds. Down-regulation of any of two Ras-dva genes in fish and frog resulted in a retardation of regeneration accompanied by down-regulation of the regeneration marker genes. On the other hand, Ras-dva over-expression in tadpoles’ tails restores regeneration capacity during the refractory period when regeneration is blocked due to natural reasons. Thus our data on Ras-dva genes, which were eliminated in amniotes but play role in anamniotes regeneration regulation, satisfy our hypothesis.
format	article
author	Anastasiya S. Ivanova Daria D. Korotkova Galina V. Ermakova Natalia Yu. Martynova Andrey G. Zaraisky Maria B. Tereshina
author_facet	Anastasiya S. Ivanova Daria D. Korotkova Galina V. Ermakova Natalia Yu. Martynova Andrey G. Zaraisky Maria B. Tereshina
author_sort	Anastasiya S. Ivanova
title	Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes
title_short	Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes
title_full	Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes
title_fullStr	Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes
title_full_unstemmed	Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes
title_sort	ras-dva small gtpases lost during evolution of amniotes regulate regeneration in anamniotes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/baab8ae0beba4a9c944d2371d79d12a6
work_keys_str_mv	AT anastasiyasivanova rasdvasmallgtpaseslostduringevolutionofamniotesregulateregenerationinanamniotes AT dariadkorotkova rasdvasmallgtpaseslostduringevolutionofamniotesregulateregenerationinanamniotes AT galinavermakova rasdvasmallgtpaseslostduringevolutionofamniotesregulateregenerationinanamniotes AT nataliayumartynova rasdvasmallgtpaseslostduringevolutionofamniotesregulateregenerationinanamniotes AT andreygzaraisky rasdvasmallgtpaseslostduringevolutionofamniotesregulateregenerationinanamniotes AT mariabtereshina rasdvasmallgtpaseslostduringevolutionofamniotesregulateregenerationinanamniotes
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Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes

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