<i>Plicosepalus acacia</i> Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling

<i>Plicosepalus acacia</i> (Fam. Loranthaceae) has been reported to possess hypoglycemic, antioxidant, antimicrobial, and anti-inflammatory effects. Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) analysis revealed the presence of a high content of polyphenolic co...

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Autores principales:	Asmaa R. Abdel-Hamed, Eman T. Mehanna, Reem M. Hazem, Jihan M. Badr, Dina M. Abo-Elmatty, Maged S. Abdel-Kader, Marwa S. Goda
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Plicosepalus acacia</i> LC-MS/MS methyl gallate quercetin HPTLC hind limb ischemia Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/baae916810db417bbfb7b56dd076dd00
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spelling	oai:doaj.org-article:baae916810db417bbfb7b56dd076dd002021-11-25T16:26:34Z<i>Plicosepalus acacia</i> Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling10.3390/antiox101117012076-3921https://doaj.org/article/baae916810db417bbfb7b56dd076dd002021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1701https://doaj.org/toc/2076-3921<i>Plicosepalus acacia</i> (Fam. Loranthaceae) has been reported to possess hypoglycemic, antioxidant, antimicrobial, and anti-inflammatory effects. Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) analysis revealed the presence of a high content of polyphenolic compounds that are attributed to the therapeutic effects of the crude extract. In addition, methyl gallate and quercetin were detected as major phytomedicinal agents at concentrations of 1.7% and 0.062 g%, respectively, using high-performance thin layer chromatography (HPTLC). The present study investigated the effect of the <i>P. acacia</i> extract and its isolated compounds, methyl gallate and quercetin, on hind limb ischemia induced in type 1 diabetic rats. Histopathological examination revealed that treatment with <i>P. acacia</i> extract, methyl gallate, and quercetin decreased degenerative changes and inflammation in the ischemic muscle. Further biochemical assessment of the hind limb tissue showed decreased oxidative stress, increased levels of nitric oxide and endothelial nitric oxide synthase (eNOS), and enhancement of the levels of heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) in the groups treated with methyl gallate and quercetin. Expression levels of hypoxia inducible factor-1 alpha (HIF-1α), VEGF, fibroblast growth factor-2 (FGF-2), and miR-146a were upregulated in the muscle tissue of methyl gallate- and quercetin-treated groups along with downregulation of nuclear factor kappa B (NF-κB). In conclusion, <i>P. acacia</i> extract and its isolated compounds, methyl gallate and quercetin, mediated therapeutic angiogenesis in diabetic hind limb ischemia.Asmaa R. Abdel-HamedEman T. MehannaReem M. HazemJihan M. BadrDina M. Abo-ElmattyMaged S. Abdel-KaderMarwa S. GodaMDPI AGarticle<i>Plicosepalus acacia</i>LC-MS/MSmethyl gallatequercetinHPTLChind limb ischemiaTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1701, p 1701 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Plicosepalus acacia</i> LC-MS/MS methyl gallate quercetin HPTLC hind limb ischemia Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Plicosepalus acacia</i> LC-MS/MS methyl gallate quercetin HPTLC hind limb ischemia Therapeutics. Pharmacology RM1-950 Asmaa R. Abdel-Hamed Eman T. Mehanna Reem M. Hazem Jihan M. Badr Dina M. Abo-Elmatty Maged S. Abdel-Kader Marwa S. Goda <i>Plicosepalus acacia</i> Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling
description	<i>Plicosepalus acacia</i> (Fam. Loranthaceae) has been reported to possess hypoglycemic, antioxidant, antimicrobial, and anti-inflammatory effects. Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) analysis revealed the presence of a high content of polyphenolic compounds that are attributed to the therapeutic effects of the crude extract. In addition, methyl gallate and quercetin were detected as major phytomedicinal agents at concentrations of 1.7% and 0.062 g%, respectively, using high-performance thin layer chromatography (HPTLC). The present study investigated the effect of the <i>P. acacia</i> extract and its isolated compounds, methyl gallate and quercetin, on hind limb ischemia induced in type 1 diabetic rats. Histopathological examination revealed that treatment with <i>P. acacia</i> extract, methyl gallate, and quercetin decreased degenerative changes and inflammation in the ischemic muscle. Further biochemical assessment of the hind limb tissue showed decreased oxidative stress, increased levels of nitric oxide and endothelial nitric oxide synthase (eNOS), and enhancement of the levels of heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) in the groups treated with methyl gallate and quercetin. Expression levels of hypoxia inducible factor-1 alpha (HIF-1α), VEGF, fibroblast growth factor-2 (FGF-2), and miR-146a were upregulated in the muscle tissue of methyl gallate- and quercetin-treated groups along with downregulation of nuclear factor kappa B (NF-κB). In conclusion, <i>P. acacia</i> extract and its isolated compounds, methyl gallate and quercetin, mediated therapeutic angiogenesis in diabetic hind limb ischemia.
format	article
author	Asmaa R. Abdel-Hamed Eman T. Mehanna Reem M. Hazem Jihan M. Badr Dina M. Abo-Elmatty Maged S. Abdel-Kader Marwa S. Goda
author_facet	Asmaa R. Abdel-Hamed Eman T. Mehanna Reem M. Hazem Jihan M. Badr Dina M. Abo-Elmatty Maged S. Abdel-Kader Marwa S. Goda
author_sort	Asmaa R. Abdel-Hamed
title	<i>Plicosepalus acacia</i> Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling
title_short	<i>Plicosepalus acacia</i> Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling
title_full	<i>Plicosepalus acacia</i> Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling
title_fullStr	<i>Plicosepalus acacia</i> Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling
title_full_unstemmed	<i>Plicosepalus acacia</i> Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling
title_sort	<i>plicosepalus acacia</i> extract and its major constituents, methyl gallate and quercetin, potentiate therapeutic angiogenesis in diabetic hind limb ischemia: hptlc quantification and lc-ms/ms metabolic profiling
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/baae916810db417bbfb7b56dd076dd00
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<i>Plicosepalus acacia</i> Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling

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