Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells

Adipose-derived mesenchymal stem cells (ADSCs), which tended to neurogenically differentiate spontaneously after achieving high confluence, were observed. Human ADSCs reaching 80% confluence were cultured in DMEM without an inducing factor for 24 h and then maintained in DMEM plus 1% FBS medium for...

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Autores principales:	Szu-Hsien Wu, Yu-Ting Liao, Chi-Han Huang, Yi-Chou Chen, En-Rung Chiang, Jung-Pan Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipose-derived mesenchymal stem cells (ADSCs) bone marrow-derived mesenchymal stem cells (BMSCs) confluence-initiated differentiation neurogenic differentiation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/baaf57e03798409bb146b8a6b33fb818
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spelling	oai:doaj.org-article:baaf57e03798409bb146b8a6b33fb8182021-11-25T16:48:20ZComparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells10.3390/biomedicines91115032227-9059https://doaj.org/article/baaf57e03798409bb146b8a6b33fb8182021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1503https://doaj.org/toc/2227-9059Adipose-derived mesenchymal stem cells (ADSCs), which tended to neurogenically differentiate spontaneously after achieving high confluence, were observed. Human ADSCs reaching 80% confluence were cultured in DMEM without an inducing factor for 24 h and then maintained in DMEM plus 1% FBS medium for 7 days. The neurogenic, adipogenic, and osteogenic genes of the factor-induced and confluence-initiated differentiation of the ADSCs and bone marrow-derived mesenchymal stem cells (BMSCs) at passages 3 to 5 were determined and compared using RT-qPCR, and the neurogenic differentiation was confirmed using immunofluorescent staining. In vitro tests revealed that the RNA and protein expression of neuronal markers, including class III β-tubulin (<i>TUBB3</i>), microtubule-associated protein 2 (<i>MAP2</i>), neurofilament medium polypeptide (<i>NEFM</i>), neurofilament heavy polypeptide (<i>NEFH</i>), and neurofilament light polypeptide (<i>NEFL</i>), had been enhanced in the confluence-initiated differentiation of the ADSCs. In addition, the expressions of neurotrophins, such as the nerve growth factor (<i>NGF</i>), brain-derived neurotrophic factor (<i>BDNF</i>), and glial cell-derived neurotrophic factor (<i>GDNF</i>), were also elevated in the confluence-initiated differentiation of the ADSCs. However, the confluent ADSCs did not show a tendency toward spontaneous adipogenic and osteogenic differentiation. Moreover, compared with the confluent ADSCs, the tendency of spontaneous neurogenic, adipogenic, and osteogenic differentiation of the confluent human bone marrow mesenchymal stem cells (BMSCs) was not observed. The results indicated that ADSCs had the potential to spontaneously differentiate into neuron-like cells during the confluent culture period; however, this tendency was not observed in BMSCs.Szu-Hsien WuYu-Ting LiaoChi-Han HuangYi-Chou ChenEn-Rung ChiangJung-Pan WangMDPI AGarticleadipose-derived mesenchymal stem cells (ADSCs)bone marrow-derived mesenchymal stem cells (BMSCs)confluence-initiated differentiationneurogenic differentiationBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1503, p 1503 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose-derived mesenchymal stem cells (ADSCs) bone marrow-derived mesenchymal stem cells (BMSCs) confluence-initiated differentiation neurogenic differentiation Biology (General) QH301-705.5
spellingShingle	adipose-derived mesenchymal stem cells (ADSCs) bone marrow-derived mesenchymal stem cells (BMSCs) confluence-initiated differentiation neurogenic differentiation Biology (General) QH301-705.5 Szu-Hsien Wu Yu-Ting Liao Chi-Han Huang Yi-Chou Chen En-Rung Chiang Jung-Pan Wang Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells
description	Adipose-derived mesenchymal stem cells (ADSCs), which tended to neurogenically differentiate spontaneously after achieving high confluence, were observed. Human ADSCs reaching 80% confluence were cultured in DMEM without an inducing factor for 24 h and then maintained in DMEM plus 1% FBS medium for 7 days. The neurogenic, adipogenic, and osteogenic genes of the factor-induced and confluence-initiated differentiation of the ADSCs and bone marrow-derived mesenchymal stem cells (BMSCs) at passages 3 to 5 were determined and compared using RT-qPCR, and the neurogenic differentiation was confirmed using immunofluorescent staining. In vitro tests revealed that the RNA and protein expression of neuronal markers, including class III β-tubulin (<i>TUBB3</i>), microtubule-associated protein 2 (<i>MAP2</i>), neurofilament medium polypeptide (<i>NEFM</i>), neurofilament heavy polypeptide (<i>NEFH</i>), and neurofilament light polypeptide (<i>NEFL</i>), had been enhanced in the confluence-initiated differentiation of the ADSCs. In addition, the expressions of neurotrophins, such as the nerve growth factor (<i>NGF</i>), brain-derived neurotrophic factor (<i>BDNF</i>), and glial cell-derived neurotrophic factor (<i>GDNF</i>), were also elevated in the confluence-initiated differentiation of the ADSCs. However, the confluent ADSCs did not show a tendency toward spontaneous adipogenic and osteogenic differentiation. Moreover, compared with the confluent ADSCs, the tendency of spontaneous neurogenic, adipogenic, and osteogenic differentiation of the confluent human bone marrow mesenchymal stem cells (BMSCs) was not observed. The results indicated that ADSCs had the potential to spontaneously differentiate into neuron-like cells during the confluent culture period; however, this tendency was not observed in BMSCs.
format	article
author	Szu-Hsien Wu Yu-Ting Liao Chi-Han Huang Yi-Chou Chen En-Rung Chiang Jung-Pan Wang
author_facet	Szu-Hsien Wu Yu-Ting Liao Chi-Han Huang Yi-Chou Chen En-Rung Chiang Jung-Pan Wang
author_sort	Szu-Hsien Wu
title	Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells
title_short	Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells
title_full	Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells
title_fullStr	Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells
title_full_unstemmed	Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells
title_sort	comparison of the confluence-initiated neurogenic differentiation tendency of adipose-derived and bone marrow-derived mesenchymal stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/baaf57e03798409bb146b8a6b33fb818
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Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells

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