Application Potential of Cyanide Hydratase from <i>Exidia glandulosa</i>: Free Cyanide Removal from Simulated Industrial Effluents

Industries such as mining, cokemaking, (petro)chemical and electroplating produce effluents that contain free cyanide (fCN = HCN + CN<sup>−</sup>). Currently, fCN is mainly removed by (physico)chemical methods or by biotreatment with activated sludge. Cyanide hydratases (CynHs) (EC 4.2.1...

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Autores principales:	Anastasia Sedova, Lenka Rucká, Pavla Bojarová, Michaela Glozlová, Petr Novotný, Barbora Křístková, Miroslav Pátek, Ludmila Martínková
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biocatalyst cyanide hydratase nitrilase <i>Exidia glandulosa</i> industrial effluent cokemaking Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/baafc1c9cd8c4b88b5db5a47377589c9
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spelling	oai:doaj.org-article:baafc1c9cd8c4b88b5db5a47377589c92021-11-25T17:06:58ZApplication Potential of Cyanide Hydratase from <i>Exidia glandulosa</i>: Free Cyanide Removal from Simulated Industrial Effluents10.3390/catal111114102073-4344https://doaj.org/article/baafc1c9cd8c4b88b5db5a47377589c92021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4344/11/11/1410https://doaj.org/toc/2073-4344Industries such as mining, cokemaking, (petro)chemical and electroplating produce effluents that contain free cyanide (fCN = HCN + CN<sup>−</sup>). Currently, fCN is mainly removed by (physico)chemical methods or by biotreatment with activated sludge. Cyanide hydratases (CynHs) (EC 4.2.1.66), which convert fCN to the much less toxic formamide, have been considered for a mild approach to wastewater decyanation. However, few data are available to evaluate the application potential of CynHs. In this study, we used a new CynH from <i>Exidia glandulosa</i> (protein KZV92691.1 designated NitEg by us), which was overproduced in <i>Escherichia coli</i>. The purified NitEg was highly active for fCN with 784 U/mg protein, <i>k</i><sub>cat</sub> 927/s and <i>k</i><sub>cat</sub>/<i>K</i><sub>M</sub> 42/s/mM. It exhibited optimal activities at pH approximately 6–9 and 40–45 °C. It was quite stable in this pH range, and retained approximately 40% activity at 37 °C after 1 day. Silver and copper ions (1 mM) decreased its activity by 30–40%. The removal of 98–100% fCN was achieved for 0.6–100 mM fCN. Moreover, thiocyanate, sulfide, ammonia or phenol added in amounts typical of industrial effluents did not significantly reduce the fCN conversion, while electroplating effluents may need to be diluted due to high fCN and metal content. The ease of preparation of NitEg, its high specific activity, robustness and long shelf life make it a promising biocatalyst for the detoxification of fCN.Anastasia SedovaLenka RuckáPavla BojarováMichaela GlozlováPetr NovotnýBarbora KřístkováMiroslav PátekLudmila MartínkováMDPI AGarticlebiocatalystcyanide hydratasenitrilase<i>Exidia glandulosa</i>industrial effluentcokemakingChemical technologyTP1-1185ChemistryQD1-999ENCatalysts, Vol 11, Iss 1410, p 1410 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biocatalyst cyanide hydratase nitrilase <i>Exidia glandulosa</i> industrial effluent cokemaking Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	biocatalyst cyanide hydratase nitrilase <i>Exidia glandulosa</i> industrial effluent cokemaking Chemical technology TP1-1185 Chemistry QD1-999 Anastasia Sedova Lenka Rucká Pavla Bojarová Michaela Glozlová Petr Novotný Barbora Křístková Miroslav Pátek Ludmila Martínková Application Potential of Cyanide Hydratase from <i>Exidia glandulosa</i>: Free Cyanide Removal from Simulated Industrial Effluents
description	Industries such as mining, cokemaking, (petro)chemical and electroplating produce effluents that contain free cyanide (fCN = HCN + CN<sup>−</sup>). Currently, fCN is mainly removed by (physico)chemical methods or by biotreatment with activated sludge. Cyanide hydratases (CynHs) (EC 4.2.1.66), which convert fCN to the much less toxic formamide, have been considered for a mild approach to wastewater decyanation. However, few data are available to evaluate the application potential of CynHs. In this study, we used a new CynH from <i>Exidia glandulosa</i> (protein KZV92691.1 designated NitEg by us), which was overproduced in <i>Escherichia coli</i>. The purified NitEg was highly active for fCN with 784 U/mg protein, <i>k</i><sub>cat</sub> 927/s and <i>k</i><sub>cat</sub>/<i>K</i><sub>M</sub> 42/s/mM. It exhibited optimal activities at pH approximately 6–9 and 40–45 °C. It was quite stable in this pH range, and retained approximately 40% activity at 37 °C after 1 day. Silver and copper ions (1 mM) decreased its activity by 30–40%. The removal of 98–100% fCN was achieved for 0.6–100 mM fCN. Moreover, thiocyanate, sulfide, ammonia or phenol added in amounts typical of industrial effluents did not significantly reduce the fCN conversion, while electroplating effluents may need to be diluted due to high fCN and metal content. The ease of preparation of NitEg, its high specific activity, robustness and long shelf life make it a promising biocatalyst for the detoxification of fCN.
format	article
author	Anastasia Sedova Lenka Rucká Pavla Bojarová Michaela Glozlová Petr Novotný Barbora Křístková Miroslav Pátek Ludmila Martínková
author_facet	Anastasia Sedova Lenka Rucká Pavla Bojarová Michaela Glozlová Petr Novotný Barbora Křístková Miroslav Pátek Ludmila Martínková
author_sort	Anastasia Sedova
title	Application Potential of Cyanide Hydratase from <i>Exidia glandulosa</i>: Free Cyanide Removal from Simulated Industrial Effluents
title_short	Application Potential of Cyanide Hydratase from <i>Exidia glandulosa</i>: Free Cyanide Removal from Simulated Industrial Effluents
title_full	Application Potential of Cyanide Hydratase from <i>Exidia glandulosa</i>: Free Cyanide Removal from Simulated Industrial Effluents
title_fullStr	Application Potential of Cyanide Hydratase from <i>Exidia glandulosa</i>: Free Cyanide Removal from Simulated Industrial Effluents
title_full_unstemmed	Application Potential of Cyanide Hydratase from <i>Exidia glandulosa</i>: Free Cyanide Removal from Simulated Industrial Effluents
title_sort	application potential of cyanide hydratase from <i>exidia glandulosa</i>: free cyanide removal from simulated industrial effluents
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/baafc1c9cd8c4b88b5db5a47377589c9
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Application Potential of Cyanide Hydratase from <i>Exidia glandulosa</i>: Free Cyanide Removal from Simulated Industrial Effluents

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