Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway

Abstract Neurons are highly asymmetric cells that span long distances and need to react promptly to local demands. Consequently, neuronal secretory pathway elements are distributed throughout neurites, specifically in post-synaptic compartments, to enable local protein synthesis and delivery. Whethe...

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Autores principales:	Clara-Marie Gürth, Tal M. Dankovich, Silvio O. Rizzoli, Elisa D’Este
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bab05bad81d4448b8288087e6e55003c
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spelling	oai:doaj.org-article:bab05bad81d4448b8288087e6e55003c2021-12-02T11:42:15ZSynaptic activity and strength are reflected by changes in the post-synaptic secretory pathway10.1038/s41598-020-77260-22045-2322https://doaj.org/article/bab05bad81d4448b8288087e6e55003c2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77260-2https://doaj.org/toc/2045-2322Abstract Neurons are highly asymmetric cells that span long distances and need to react promptly to local demands. Consequently, neuronal secretory pathway elements are distributed throughout neurites, specifically in post-synaptic compartments, to enable local protein synthesis and delivery. Whether and how changes in local synaptic activity correlate to post-synaptic secretory elements is still unclear. To assess this, we used STED nanoscopy and automated quantitative image analysis of post-synaptic markers of the endoplasmic reticulum, ER-Golgi intermediate compartment, trans-Golgi network, and spine apparatus. We found that the distribution of these proteins was dependent on pre-synaptic activity, measured as the amount of recycling vesicles. Moreover, their abundance correlated to both pre- and post-synaptic markers of synaptic strength. Overall, the results suggest that in small, low-activity synapses the secretory pathway components are tightly clustered in the synaptic area, presumably to enable rapid local responses, while bigger synapses utilise secretory machinery components from larger, more diffuse areas.Clara-Marie GürthTal M. DankovichSilvio O. RizzoliElisa D’EsteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Clara-Marie Gürth Tal M. Dankovich Silvio O. Rizzoli Elisa D’Este Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway
description	Abstract Neurons are highly asymmetric cells that span long distances and need to react promptly to local demands. Consequently, neuronal secretory pathway elements are distributed throughout neurites, specifically in post-synaptic compartments, to enable local protein synthesis and delivery. Whether and how changes in local synaptic activity correlate to post-synaptic secretory elements is still unclear. To assess this, we used STED nanoscopy and automated quantitative image analysis of post-synaptic markers of the endoplasmic reticulum, ER-Golgi intermediate compartment, trans-Golgi network, and spine apparatus. We found that the distribution of these proteins was dependent on pre-synaptic activity, measured as the amount of recycling vesicles. Moreover, their abundance correlated to both pre- and post-synaptic markers of synaptic strength. Overall, the results suggest that in small, low-activity synapses the secretory pathway components are tightly clustered in the synaptic area, presumably to enable rapid local responses, while bigger synapses utilise secretory machinery components from larger, more diffuse areas.
format	article
author	Clara-Marie Gürth Tal M. Dankovich Silvio O. Rizzoli Elisa D’Este
author_facet	Clara-Marie Gürth Tal M. Dankovich Silvio O. Rizzoli Elisa D’Este
author_sort	Clara-Marie Gürth
title	Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway
title_short	Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway
title_full	Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway
title_fullStr	Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway
title_full_unstemmed	Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway
title_sort	synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/bab05bad81d4448b8288087e6e55003c
work_keys_str_mv	AT claramariegurth synapticactivityandstrengtharereflectedbychangesinthepostsynapticsecretorypathway AT talmdankovich synapticactivityandstrengtharereflectedbychangesinthepostsynapticsecretorypathway AT silvioorizzoli synapticactivityandstrengtharereflectedbychangesinthepostsynapticsecretorypathway AT elisadeste synapticactivityandstrengtharereflectedbychangesinthepostsynapticsecretorypathway
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Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway

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