Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues.

Microtubules are dynamic polymers of GTP- and GDP-tubulin that undergo stochastic transitions between growing and shrinking phases. Rescues, the conversion from shrinking to growing, have recently been proposed to be to the result of regrowth at GTP-tubulin islands within the lattice of growing micr...

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Autores principales:	Carolina Tropini, Elizabeth A Roth, Marija Zanic, Melissa K Gardner, Jonathon Howard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/babdd7276152472abb62a1882c45aeed
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spelling	oai:doaj.org-article:babdd7276152472abb62a1882c45aeed2021-11-18T07:30:10ZIslands containing slowly hydrolyzable GTP analogs promote microtubule rescues.1932-620310.1371/journal.pone.0030103https://doaj.org/article/babdd7276152472abb62a1882c45aeed2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22272281/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Microtubules are dynamic polymers of GTP- and GDP-tubulin that undergo stochastic transitions between growing and shrinking phases. Rescues, the conversion from shrinking to growing, have recently been proposed to be to the result of regrowth at GTP-tubulin islands within the lattice of growing microtubules. By introducing mixed GTP/GDP/GMPCPP (GXP) regions within the lattice of dynamic microtubules, we reconstituted GXP islands in vitro (GMPCPP is the slowly hydrolyzable GTP analog guanosine-5'-[(α,β)-methyleno]triphosphate). We found that such islands could reproducibly induce rescues and that the probability of rescue correlated with both the size of the island and the percentage of GMPCPP-tubulin within the island. The islands slowed the depolymerization rate of shortening microtubules and promoted regrowth more readily than GMPCPP seeds. Together, these findings provide new mechanistic insights supporting the possibility that rescues could be triggered by enriched GTP-tubulin regions and present a new tool for studying such rescue events in vitro.Carolina TropiniElizabeth A RothMarija ZanicMelissa K GardnerJonathon HowardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30103 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carolina Tropini Elizabeth A Roth Marija Zanic Melissa K Gardner Jonathon Howard Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues.
description	Microtubules are dynamic polymers of GTP- and GDP-tubulin that undergo stochastic transitions between growing and shrinking phases. Rescues, the conversion from shrinking to growing, have recently been proposed to be to the result of regrowth at GTP-tubulin islands within the lattice of growing microtubules. By introducing mixed GTP/GDP/GMPCPP (GXP) regions within the lattice of dynamic microtubules, we reconstituted GXP islands in vitro (GMPCPP is the slowly hydrolyzable GTP analog guanosine-5'-[(α,β)-methyleno]triphosphate). We found that such islands could reproducibly induce rescues and that the probability of rescue correlated with both the size of the island and the percentage of GMPCPP-tubulin within the island. The islands slowed the depolymerization rate of shortening microtubules and promoted regrowth more readily than GMPCPP seeds. Together, these findings provide new mechanistic insights supporting the possibility that rescues could be triggered by enriched GTP-tubulin regions and present a new tool for studying such rescue events in vitro.
format	article
author	Carolina Tropini Elizabeth A Roth Marija Zanic Melissa K Gardner Jonathon Howard
author_facet	Carolina Tropini Elizabeth A Roth Marija Zanic Melissa K Gardner Jonathon Howard
author_sort	Carolina Tropini
title	Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues.
title_short	Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues.
title_full	Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues.
title_fullStr	Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues.
title_full_unstemmed	Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues.
title_sort	islands containing slowly hydrolyzable gtp analogs promote microtubule rescues.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/babdd7276152472abb62a1882c45aeed
work_keys_str_mv	AT carolinatropini islandscontainingslowlyhydrolyzablegtpanalogspromotemicrotubulerescues AT elizabetharoth islandscontainingslowlyhydrolyzablegtpanalogspromotemicrotubulerescues AT marijazanic islandscontainingslowlyhydrolyzablegtpanalogspromotemicrotubulerescues AT melissakgardner islandscontainingslowlyhydrolyzablegtpanalogspromotemicrotubulerescues AT jonathonhoward islandscontainingslowlyhydrolyzablegtpanalogspromotemicrotubulerescues
_version_	1718423372367396864

Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues.

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