Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.

<h4>Background</h4>Tuberculosis (TB) remains a global threat in the 21st century. Traditional studies of the disease are focused on the single pathogen Mycobacterium tuberculosis. Recent studies have revealed associations of some diseases with an imbalance in the microbial community. Cha...

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Autores principales:	Man Kit Cheung, Wai Yip Lam, Wendy Yin Wan Fung, Patrick Tik Wan Law, Chun Hang Au, Wenyan Nong, Kai Man Kam, Hoi Shan Kwan, Stephen Kwok Wing Tsui
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bac4179b987e46fbb0dd0732e437c2aa
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spelling	oai:doaj.org-article:bac4179b987e46fbb0dd0732e437c2aa2021-11-18T08:00:01ZSputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.1932-620310.1371/journal.pone.0054574https://doaj.org/article/bac4179b987e46fbb0dd0732e437c2aa2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23365674/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Tuberculosis (TB) remains a global threat in the 21st century. Traditional studies of the disease are focused on the single pathogen Mycobacterium tuberculosis. Recent studies have revealed associations of some diseases with an imbalance in the microbial community. Characterization of the TB microbiota could allow a better understanding of the disease.<h4>Methodology/principal findings</h4>Here, the sputum microbiota in TB infection was examined by using 16S rRNA pyrosequencing. A total of 829,873 high-quality sequencing reads were generated from 22 TB and 14 control sputum samples. Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, and Fusobacteria were the five major bacterial phyla recovered, which together composed over 98% of the microbial community. Proteobacteria and Bacteroidetes were more represented in the TB samples and Firmicutes was more predominant in the controls. Sixteen major bacterial genera were recovered. Streptococcus, Neisseria and Prevotella were the most predominant genera, which were dominated by several operational taxonomic units grouped at a 97% similarity level. Actinomyces, Fusobacterium, Leptotrichia, Prevotella, Streptococcus, and Veillonella were found in all TB samples, possibly representing the core genera in TB sputum microbiota. The less represented genera Mogibacterium, Moryella and Oribacterium were enriched statistically in the TB samples, while a genus belonging to the unclassified Lactobacillales was enriched in the controls. The diversity of microbiota was similar in the TB and control samples.<h4>Conclusions/significance</h4>The composition and diversity of sputum microbiota in TB infection was characterized for the first time by using high-throughput pyrosequencing. It lays the framework for examination of potential roles played by the diverse microbiota in TB pathogenesis and progression, and could ultimately facilitate advances in TB treatment.Man Kit CheungWai Yip LamWendy Yin Wan FungPatrick Tik Wan LawChun Hang AuWenyan NongKai Man KamHoi Shan KwanStephen Kwok Wing TsuiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54574 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Man Kit Cheung Wai Yip Lam Wendy Yin Wan Fung Patrick Tik Wan Law Chun Hang Au Wenyan Nong Kai Man Kam Hoi Shan Kwan Stephen Kwok Wing Tsui Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.
description	<h4>Background</h4>Tuberculosis (TB) remains a global threat in the 21st century. Traditional studies of the disease are focused on the single pathogen Mycobacterium tuberculosis. Recent studies have revealed associations of some diseases with an imbalance in the microbial community. Characterization of the TB microbiota could allow a better understanding of the disease.<h4>Methodology/principal findings</h4>Here, the sputum microbiota in TB infection was examined by using 16S rRNA pyrosequencing. A total of 829,873 high-quality sequencing reads were generated from 22 TB and 14 control sputum samples. Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, and Fusobacteria were the five major bacterial phyla recovered, which together composed over 98% of the microbial community. Proteobacteria and Bacteroidetes were more represented in the TB samples and Firmicutes was more predominant in the controls. Sixteen major bacterial genera were recovered. Streptococcus, Neisseria and Prevotella were the most predominant genera, which were dominated by several operational taxonomic units grouped at a 97% similarity level. Actinomyces, Fusobacterium, Leptotrichia, Prevotella, Streptococcus, and Veillonella were found in all TB samples, possibly representing the core genera in TB sputum microbiota. The less represented genera Mogibacterium, Moryella and Oribacterium were enriched statistically in the TB samples, while a genus belonging to the unclassified Lactobacillales was enriched in the controls. The diversity of microbiota was similar in the TB and control samples.<h4>Conclusions/significance</h4>The composition and diversity of sputum microbiota in TB infection was characterized for the first time by using high-throughput pyrosequencing. It lays the framework for examination of potential roles played by the diverse microbiota in TB pathogenesis and progression, and could ultimately facilitate advances in TB treatment.
format	article
author	Man Kit Cheung Wai Yip Lam Wendy Yin Wan Fung Patrick Tik Wan Law Chun Hang Au Wenyan Nong Kai Man Kam Hoi Shan Kwan Stephen Kwok Wing Tsui
author_facet	Man Kit Cheung Wai Yip Lam Wendy Yin Wan Fung Patrick Tik Wan Law Chun Hang Au Wenyan Nong Kai Man Kam Hoi Shan Kwan Stephen Kwok Wing Tsui
author_sort	Man Kit Cheung
title	Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.
title_short	Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.
title_full	Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.
title_fullStr	Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.
title_full_unstemmed	Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.
title_sort	sputum microbiota in tuberculosis as revealed by 16s rrna pyrosequencing.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/bac4179b987e46fbb0dd0732e437c2aa
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Sputum microbiota in tuberculosis as revealed by 16S rRNA pyrosequencing.

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