Minimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes

Abstract Chronically implanted neural probes are powerful tools to decode brain activity however, recording population and spiking activity over long periods remains a major challenge. Here, we designed and fabricated flexible intracortical Michigan-style arrays with a shank cross-section per electr...

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Autores principales:	Kagithiri Srikantharajah, Renata Medinaceli Quintela, Kerstin Doerenkamp, Björn M. Kampa, Simon Musall, Markus Rothermel, Andreas Offenhäusser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bac99a47bfce4834b3654ba50618778a
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spelling	oai:doaj.org-article:bac99a47bfce4834b3654ba50618778a2021-12-02T15:14:37ZMinimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes10.1038/s41598-021-97940-x2045-2322https://doaj.org/article/bac99a47bfce4834b3654ba50618778a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97940-xhttps://doaj.org/toc/2045-2322Abstract Chronically implanted neural probes are powerful tools to decode brain activity however, recording population and spiking activity over long periods remains a major challenge. Here, we designed and fabricated flexible intracortical Michigan-style arrays with a shank cross-section per electrode of 250 μm $$^2$$ 2 utilizing the polymer paryleneC with the goal to improve the immune acceptance. As flexible neural probes are unable to penetrate the brain due to the low buckling force threshold, a tissue-friendly insertion system was developed by reducing the effective shank length. The insertion strategy enabled the implantation of the four, bare, flexible shanks up to 2 mm into the mouse brain without increasing the implantation footprint and therefore, minimizing the acute trauma. In acute recordings from the mouse somatosensory cortex and the olfactory bulb, we demonstrated that the flexible probes were able to simultaneously detect local field potentials as well as single and multi-unit activity. Additionally, the flexible arrays outperformed stiff probes with respect to yield of single unit activity. Following the successful in vivo validation, we further improved the microfabrication towards a double-metal-layer process, and were able to double the number of electrodes per shank by keeping the shank width resulting in a cross-section per electrode of 118 μm $$^2$$ 2 .Kagithiri SrikantharajahRenata Medinaceli QuintelaKerstin DoerenkampBjörn M. KampaSimon MusallMarkus RothermelAndreas OffenhäusserNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kagithiri Srikantharajah Renata Medinaceli Quintela Kerstin Doerenkamp Björn M. Kampa Simon Musall Markus Rothermel Andreas Offenhäusser Minimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes
description	Abstract Chronically implanted neural probes are powerful tools to decode brain activity however, recording population and spiking activity over long periods remains a major challenge. Here, we designed and fabricated flexible intracortical Michigan-style arrays with a shank cross-section per electrode of 250 μm $$^2$$ 2 utilizing the polymer paryleneC with the goal to improve the immune acceptance. As flexible neural probes are unable to penetrate the brain due to the low buckling force threshold, a tissue-friendly insertion system was developed by reducing the effective shank length. The insertion strategy enabled the implantation of the four, bare, flexible shanks up to 2 mm into the mouse brain without increasing the implantation footprint and therefore, minimizing the acute trauma. In acute recordings from the mouse somatosensory cortex and the olfactory bulb, we demonstrated that the flexible probes were able to simultaneously detect local field potentials as well as single and multi-unit activity. Additionally, the flexible arrays outperformed stiff probes with respect to yield of single unit activity. Following the successful in vivo validation, we further improved the microfabrication towards a double-metal-layer process, and were able to double the number of electrodes per shank by keeping the shank width resulting in a cross-section per electrode of 118 μm $$^2$$ 2 .
format	article
author	Kagithiri Srikantharajah Renata Medinaceli Quintela Kerstin Doerenkamp Björn M. Kampa Simon Musall Markus Rothermel Andreas Offenhäusser
author_facet	Kagithiri Srikantharajah Renata Medinaceli Quintela Kerstin Doerenkamp Björn M. Kampa Simon Musall Markus Rothermel Andreas Offenhäusser
author_sort	Kagithiri Srikantharajah
title	Minimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes
title_short	Minimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes
title_full	Minimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes
title_fullStr	Minimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes
title_full_unstemmed	Minimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes
title_sort	minimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bac99a47bfce4834b3654ba50618778a
work_keys_str_mv	AT kagithirisrikantharajah minimallyinvasiveinsertionstrategyandinvivoevaluationofmultishankflexibleintracorticalprobes AT renatamedinaceliquintela minimallyinvasiveinsertionstrategyandinvivoevaluationofmultishankflexibleintracorticalprobes AT kerstindoerenkamp minimallyinvasiveinsertionstrategyandinvivoevaluationofmultishankflexibleintracorticalprobes AT bjornmkampa minimallyinvasiveinsertionstrategyandinvivoevaluationofmultishankflexibleintracorticalprobes AT simonmusall minimallyinvasiveinsertionstrategyandinvivoevaluationofmultishankflexibleintracorticalprobes AT markusrothermel minimallyinvasiveinsertionstrategyandinvivoevaluationofmultishankflexibleintracorticalprobes AT andreasoffenhausser minimallyinvasiveinsertionstrategyandinvivoevaluationofmultishankflexibleintracorticalprobes
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Minimally-invasive insertion strategy and in vivo evaluation of multi-shank flexible intracortical probes

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