Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.

Lysinibacillus sphaericus strains belonging the antigenic group H5a5b produce spores with larvicidal activity against larvae of Culex mosquitoes. C7, a new isolated strain, which presents similar biochemical characteristics and Bin toxins in their spores as the reference strain 2362, was, however, m...

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Autores principales:	Mariana Claudia Allievi, María Mercedes Palomino, Mariano Prado Acosta, Leonardo Lanati, Sandra Mónica Ruzal, Carmen Sánchez-Rivas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bacdbfd127814a098f8f69183e527489
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spelling	oai:doaj.org-article:bacdbfd127814a098f8f69183e5274892021-11-25T05:55:07ZContribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.1932-620310.1371/journal.pone.0111114https://doaj.org/article/bacdbfd127814a098f8f69183e5274892014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0111114https://doaj.org/toc/1932-6203Lysinibacillus sphaericus strains belonging the antigenic group H5a5b produce spores with larvicidal activity against larvae of Culex mosquitoes. C7, a new isolated strain, which presents similar biochemical characteristics and Bin toxins in their spores as the reference strain 2362, was, however, more active against larvae of Culex mosquitoes. The contribution of the surface layer protein (S-layer) to this behaviour was envisaged since this envelope protein has been implicated in the pathogenicity of several bacilli, and we had previously reported its association to spores. Microscopic observation by immunofluorescence detection with anti S-layer antibody in the spores confirms their attachment. S-layers and BinA and BinB toxins formed high molecular weight multimers in spores as shown by SDS-PAGE and western blot detection. Purified S-layer from both L. sphaericus C7 and 2362 strain cultures was by itself toxic against Culex sp larvae, however, that from C7 strain was also toxic against Aedes aegypti. Synergistic effect between purified S-layer and spore-crystal preparations was observed against Culex sp. and Aedes aegypti larvae. This effect was more evident with the C7 strain. In silico analyses of the S-layer sequence suggest the presence of chitin-binding and hemolytic domains. Both biochemical characteristics were detected for both S-layers strains that must justify their contribution to pathogenicity.Mariana Claudia AllieviMaría Mercedes PalominoMariano Prado AcostaLeonardo LanatiSandra Mónica RuzalCarmen Sánchez-RivasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e111114 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariana Claudia Allievi María Mercedes Palomino Mariano Prado Acosta Leonardo Lanati Sandra Mónica Ruzal Carmen Sánchez-Rivas Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.
description	Lysinibacillus sphaericus strains belonging the antigenic group H5a5b produce spores with larvicidal activity against larvae of Culex mosquitoes. C7, a new isolated strain, which presents similar biochemical characteristics and Bin toxins in their spores as the reference strain 2362, was, however, more active against larvae of Culex mosquitoes. The contribution of the surface layer protein (S-layer) to this behaviour was envisaged since this envelope protein has been implicated in the pathogenicity of several bacilli, and we had previously reported its association to spores. Microscopic observation by immunofluorescence detection with anti S-layer antibody in the spores confirms their attachment. S-layers and BinA and BinB toxins formed high molecular weight multimers in spores as shown by SDS-PAGE and western blot detection. Purified S-layer from both L. sphaericus C7 and 2362 strain cultures was by itself toxic against Culex sp larvae, however, that from C7 strain was also toxic against Aedes aegypti. Synergistic effect between purified S-layer and spore-crystal preparations was observed against Culex sp. and Aedes aegypti larvae. This effect was more evident with the C7 strain. In silico analyses of the S-layer sequence suggest the presence of chitin-binding and hemolytic domains. Both biochemical characteristics were detected for both S-layers strains that must justify their contribution to pathogenicity.
format	article
author	Mariana Claudia Allievi María Mercedes Palomino Mariano Prado Acosta Leonardo Lanati Sandra Mónica Ruzal Carmen Sánchez-Rivas
author_facet	Mariana Claudia Allievi María Mercedes Palomino Mariano Prado Acosta Leonardo Lanati Sandra Mónica Ruzal Carmen Sánchez-Rivas
author_sort	Mariana Claudia Allievi
title	Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.
title_short	Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.
title_full	Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.
title_fullStr	Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.
title_full_unstemmed	Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.
title_sort	contribution of s-layer proteins to the mosquitocidal activity of lysinibacillus sphaericus.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bacdbfd127814a098f8f69183e527489
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Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.

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