Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant

Ji-Hui Zhao,1,* Qi-Bo Zhang,1,* Bao Liu,2 Xiang-Hua Piao,1 Yu-Lu Yan,1 Xiao-Ge Hu,1 Kuan Zhou,1 Yong-Tai Zhang,1 Nian-Ping Feng1 1School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 2Anethesiology Department, Augusta University, A...

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Autores principales:	Zhao JH, Zhang QB, Liu B, Piao XH, Yan YL, Hu XG, Zhou K, Zhang YT, Feng NP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	subunit vaccine saponin adjuvant liposomes dissolving microneedle array intradermal vaccination Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bad4742b835240ba8bbde652946a280b
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spelling	oai:doaj.org-article:bad4742b835240ba8bbde652946a280b2021-12-02T06:37:58ZEnhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant1178-2013https://doaj.org/article/bad4742b835240ba8bbde652946a280b2017-07-01T00:00:00Zhttps://www.dovepress.com/enhanced-immunization-via-dissolving-microneedle-array-based-delivery--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ji-Hui Zhao,1,* Qi-Bo Zhang,1,* Bao Liu,2 Xiang-Hua Piao,1 Yu-Lu Yan,1 Xiao-Ge Hu,1 Kuan Zhou,1 Yong-Tai Zhang,1 Nian-Ping Feng1 1School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 2Anethesiology Department, Augusta University, Augusta, GA, USA *These authors contributed equally to this work Purpose: To enhance the immunogenicity of the model subunit vaccine, ovalbumin (OVA) was combined with platycodin (PD), a saponin adjuvant. To reduce the toxicity of PD, OVA, and adjuvant were loaded together into liposomes before being incorporated into a dissolving microneedle array.Methods: OVA- and PD-loaded liposomes (OVA-PD-Lipos) were prepared using the film dispersion method. Their uptake behavior, toxicity to mouse bone marrow dendritic cells (BMDCs), and hemolytic activity to rabbit red blood cells (RBCs) were evaluated. The OVA-PD-Lipos were incorporated into a dissolving microneedle array. The chemical stability of OVA and the physical stability of OVA-PD-Lipos in microneedle arrays were investigated. The immune response of Institute of Cancer Research mice and potential skin irritation reaction of rabbits to OVA-PD-Lipos-MNs were evaluated.Results: The uptake of OVA by mouse BMDCs was greatly enhanced when OVA was prepared as OVA-PD-Lipos, and in this form, the toxicity of PD was dramatically reduced. OVA was chemically stable as OVA-PD-Lipos, when OVA-PD-Lipos was incorporated into a dissolving microneedle array. Institute of Cancer Research mice treated with OVA-PD-Lipos-MNs showed a significantly enhanced immune response. PD combined with OVA elicited a balanced Th1 and Th2 humoral immune response in mice, with minimal irritation in rabbit skin.Conclusion: The dissolving microneedle array-based system is a promising delivery vehicle for subunit vaccine and its adjuvant. Keywords: subunit vaccine, saponin adjuvant, liposomes, dissolving microneedle array, intradermal vaccinationZhao JHZhang QBLiu BPiao XHYan YLHu XGZhou KZhang YTFeng NPDove Medical Pressarticlesubunit vaccinesaponin adjuvantliposomesdissolving microneedle arrayintradermal vaccinationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4763-4772 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	subunit vaccine saponin adjuvant liposomes dissolving microneedle array intradermal vaccination Medicine (General) R5-920
spellingShingle	subunit vaccine saponin adjuvant liposomes dissolving microneedle array intradermal vaccination Medicine (General) R5-920 Zhao JH Zhang QB Liu B Piao XH Yan YL Hu XG Zhou K Zhang YT Feng NP Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant
description	Ji-Hui Zhao,1,* Qi-Bo Zhang,1,* Bao Liu,2 Xiang-Hua Piao,1 Yu-Lu Yan,1 Xiao-Ge Hu,1 Kuan Zhou,1 Yong-Tai Zhang,1 Nian-Ping Feng1 1School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 2Anethesiology Department, Augusta University, Augusta, GA, USA *These authors contributed equally to this work Purpose: To enhance the immunogenicity of the model subunit vaccine, ovalbumin (OVA) was combined with platycodin (PD), a saponin adjuvant. To reduce the toxicity of PD, OVA, and adjuvant were loaded together into liposomes before being incorporated into a dissolving microneedle array.Methods: OVA- and PD-loaded liposomes (OVA-PD-Lipos) were prepared using the film dispersion method. Their uptake behavior, toxicity to mouse bone marrow dendritic cells (BMDCs), and hemolytic activity to rabbit red blood cells (RBCs) were evaluated. The OVA-PD-Lipos were incorporated into a dissolving microneedle array. The chemical stability of OVA and the physical stability of OVA-PD-Lipos in microneedle arrays were investigated. The immune response of Institute of Cancer Research mice and potential skin irritation reaction of rabbits to OVA-PD-Lipos-MNs were evaluated.Results: The uptake of OVA by mouse BMDCs was greatly enhanced when OVA was prepared as OVA-PD-Lipos, and in this form, the toxicity of PD was dramatically reduced. OVA was chemically stable as OVA-PD-Lipos, when OVA-PD-Lipos was incorporated into a dissolving microneedle array. Institute of Cancer Research mice treated with OVA-PD-Lipos-MNs showed a significantly enhanced immune response. PD combined with OVA elicited a balanced Th1 and Th2 humoral immune response in mice, with minimal irritation in rabbit skin.Conclusion: The dissolving microneedle array-based system is a promising delivery vehicle for subunit vaccine and its adjuvant. Keywords: subunit vaccine, saponin adjuvant, liposomes, dissolving microneedle array, intradermal vaccination
format	article
author	Zhao JH Zhang QB Liu B Piao XH Yan YL Hu XG Zhou K Zhang YT Feng NP
author_facet	Zhao JH Zhang QB Liu B Piao XH Yan YL Hu XG Zhou K Zhang YT Feng NP
author_sort	Zhao JH
title	Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant
title_short	Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant
title_full	Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant
title_fullStr	Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant
title_full_unstemmed	Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant
title_sort	enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/bad4742b835240ba8bbde652946a280b
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Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant

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