Investigation of neuronal pathfinding and construction of artificial neuronal networks on 3D-arranged porous fibrillar scaffolds with controlled geometry

Abstract Herein, we investigated the neurite pathfinding on electrospun microfibers with various fiber densities, diameters, and microbead islands, and demonstrated the development of 3D connected artificial neuronal network within a nanofiber-microbead-based porous scaffold. The primary culture of...

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Autores principales:	Dongyoon Kim, Seong-Min Kim, Seyeong Lee, Myung-Han Yoon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bad9573b41bb40a289c3c04f1d31a964
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spelling	oai:doaj.org-article:bad9573b41bb40a289c3c04f1d31a9642021-12-02T12:30:27ZInvestigation of neuronal pathfinding and construction of artificial neuronal networks on 3D-arranged porous fibrillar scaffolds with controlled geometry10.1038/s41598-017-08231-32045-2322https://doaj.org/article/bad9573b41bb40a289c3c04f1d31a9642017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08231-3https://doaj.org/toc/2045-2322Abstract Herein, we investigated the neurite pathfinding on electrospun microfibers with various fiber densities, diameters, and microbead islands, and demonstrated the development of 3D connected artificial neuronal network within a nanofiber-microbead-based porous scaffold. The primary culture of rat hippocampal embryonic neurons was deposited on geometry-controlled polystyrene (PS) fiber scaffolds while growth cone morphology, neurite outgrowth patterns, and focal adhesion protein expression were cautiously examined by microscopic imaging of immunostained and live neuronal cells derived from actin-GFP transgenic mice. It was demonstrated that the neurite outgrowth was guided by the overall microfiber orientation, but the increase in fiber density induced the neurite path alteration, thus, the reduction in neurite linearity. Indeed, we experimentally confirmed that growth cone could migrate to a neighboring, but, spatially disconnected microfiber by spontaneous filopodium extrusion, which is possibly responsible for the observed neurite steering. Furthermore, thinner microfiber scaffolds showed more pronounced expression of focal adhesion proteins than thicker ones, suggesting that the neuron-microfiber interaction can be delicately modulated by the underlying microfiber geometry. Finally, 3D connected functional neuronal networks were successfully constructed using PS nanofiber-microbead scaffolds where enhanced porosity and vertical fiber orientation permitted cell body inclusion within the scaffold and substantial neurite outgrowth in a vertical direction, respectively.Dongyoon KimSeong-Min KimSeyeong LeeMyung-Han YoonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dongyoon Kim Seong-Min Kim Seyeong Lee Myung-Han Yoon Investigation of neuronal pathfinding and construction of artificial neuronal networks on 3D-arranged porous fibrillar scaffolds with controlled geometry
description	Abstract Herein, we investigated the neurite pathfinding on electrospun microfibers with various fiber densities, diameters, and microbead islands, and demonstrated the development of 3D connected artificial neuronal network within a nanofiber-microbead-based porous scaffold. The primary culture of rat hippocampal embryonic neurons was deposited on geometry-controlled polystyrene (PS) fiber scaffolds while growth cone morphology, neurite outgrowth patterns, and focal adhesion protein expression were cautiously examined by microscopic imaging of immunostained and live neuronal cells derived from actin-GFP transgenic mice. It was demonstrated that the neurite outgrowth was guided by the overall microfiber orientation, but the increase in fiber density induced the neurite path alteration, thus, the reduction in neurite linearity. Indeed, we experimentally confirmed that growth cone could migrate to a neighboring, but, spatially disconnected microfiber by spontaneous filopodium extrusion, which is possibly responsible for the observed neurite steering. Furthermore, thinner microfiber scaffolds showed more pronounced expression of focal adhesion proteins than thicker ones, suggesting that the neuron-microfiber interaction can be delicately modulated by the underlying microfiber geometry. Finally, 3D connected functional neuronal networks were successfully constructed using PS nanofiber-microbead scaffolds where enhanced porosity and vertical fiber orientation permitted cell body inclusion within the scaffold and substantial neurite outgrowth in a vertical direction, respectively.
format	article
author	Dongyoon Kim Seong-Min Kim Seyeong Lee Myung-Han Yoon
author_facet	Dongyoon Kim Seong-Min Kim Seyeong Lee Myung-Han Yoon
author_sort	Dongyoon Kim
title	Investigation of neuronal pathfinding and construction of artificial neuronal networks on 3D-arranged porous fibrillar scaffolds with controlled geometry
title_short	Investigation of neuronal pathfinding and construction of artificial neuronal networks on 3D-arranged porous fibrillar scaffolds with controlled geometry
title_full	Investigation of neuronal pathfinding and construction of artificial neuronal networks on 3D-arranged porous fibrillar scaffolds with controlled geometry
title_fullStr	Investigation of neuronal pathfinding and construction of artificial neuronal networks on 3D-arranged porous fibrillar scaffolds with controlled geometry
title_full_unstemmed	Investigation of neuronal pathfinding and construction of artificial neuronal networks on 3D-arranged porous fibrillar scaffolds with controlled geometry
title_sort	investigation of neuronal pathfinding and construction of artificial neuronal networks on 3d-arranged porous fibrillar scaffolds with controlled geometry
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bad9573b41bb40a289c3c04f1d31a964
work_keys_str_mv	AT dongyoonkim investigationofneuronalpathfindingandconstructionofartificialneuronalnetworkson3darrangedporousfibrillarscaffoldswithcontrolledgeometry AT seongminkim investigationofneuronalpathfindingandconstructionofartificialneuronalnetworkson3darrangedporousfibrillarscaffoldswithcontrolledgeometry AT seyeonglee investigationofneuronalpathfindingandconstructionofartificialneuronalnetworkson3darrangedporousfibrillarscaffoldswithcontrolledgeometry AT myunghanyoon investigationofneuronalpathfindingandconstructionofartificialneuronalnetworkson3darrangedporousfibrillarscaffoldswithcontrolledgeometry
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Investigation of neuronal pathfinding and construction of artificial neuronal networks on 3D-arranged porous fibrillar scaffolds with controlled geometry

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