A Reverse Genetics Platform That Spans the Zika Virus Family Tree

ABSTRACT Zika virus (ZIKV), a mosquito-borne flavivirus discovered in 1947, has only recently caused large outbreaks and emerged as a significant human pathogen. In 2015, ZIKV was detected in Brazil, and the resulting epidemic has spread throughout the Western Hemisphere. Severe complications from Z...

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Autores principales:	Douglas G. Widman, Ellen Young, Boyd L. Yount, Kenneth S. Plante, Emily N. Gallichotte, Derek L. Carbaugh, Kayla M. Peck, Jessica Plante, Jesica Swanstrom, Mark T. Heise, Helen M. Lazear, Ralph S. Baric
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/badd76acfe644f0ea9b1b6e9afa5d37b
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spelling	oai:doaj.org-article:badd76acfe644f0ea9b1b6e9afa5d37b2021-11-15T15:51:00ZA Reverse Genetics Platform That Spans the Zika Virus Family Tree10.1128/mBio.02014-162150-7511https://doaj.org/article/badd76acfe644f0ea9b1b6e9afa5d37b2017-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02014-16https://doaj.org/toc/2150-7511ABSTRACT Zika virus (ZIKV), a mosquito-borne flavivirus discovered in 1947, has only recently caused large outbreaks and emerged as a significant human pathogen. In 2015, ZIKV was detected in Brazil, and the resulting epidemic has spread throughout the Western Hemisphere. Severe complications from ZIKV infection include neurological disorders such as Guillain-Barré syndrome in adults and a variety of fetal abnormalities, including microcephaly, blindness, placental insufficiency, and fetal demise. There is an urgent need for tools and reagents to study the pathogenesis of epidemic ZIKV and for testing vaccines and antivirals. Using a reverse genetics platform, we generated six ZIKV infectious clones and derivative viruses representing diverse temporal and geographic origins. These include three versions of MR766, the prototype 1947 strain (with and without a glycosylation site in the envelope protein), and H/PF/2013, a 2013 human isolate from French Polynesia representative of the virus introduced to Brazil. In the course of synthesizing a clone of a circulating Brazilian strain, phylogenetic studies identified two distinct ZIKV clades in Brazil. We reconstructed viable clones of strains SPH2015 and BeH819015, representing ancestral members of each clade. We assessed recombinant virus replication, binding to monoclonal antibodies, and virulence in mice. This panel of molecular clones and recombinant virus isolates will enable targeted studies of viral determinants of pathogenesis, adaptation, and evolution, as well as the rational attenuation of contemporary outbreak strains to facilitate the design of vaccines and therapeutics. IMPORTANCE Viral emergence is a poorly understood process as evidenced by the sudden emergence of Zika virus in Latin America and the Caribbean. Malleable reagents that both predate and span an expanding epidemic are key to understanding the virologic determinants that regulate pathogenesis and transmission. We have generated representative cDNA molecular clones and recombinant viruses that span the known ZIKV family tree, including early Brazilian isolates. Recombinant viruses replicated efficiently in cell culture and were pathogenic in immunodeficient mice, providing a genetic platform for rational vaccine and therapeutic design.Douglas G. WidmanEllen YoungBoyd L. YountKenneth S. PlanteEmily N. GallichotteDerek L. CarbaughKayla M. PeckJessica PlanteJesica SwanstromMark T. HeiseHelen M. LazearRalph S. BaricAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 8, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Douglas G. Widman Ellen Young Boyd L. Yount Kenneth S. Plante Emily N. Gallichotte Derek L. Carbaugh Kayla M. Peck Jessica Plante Jesica Swanstrom Mark T. Heise Helen M. Lazear Ralph S. Baric A Reverse Genetics Platform That Spans the Zika Virus Family Tree
description	ABSTRACT Zika virus (ZIKV), a mosquito-borne flavivirus discovered in 1947, has only recently caused large outbreaks and emerged as a significant human pathogen. In 2015, ZIKV was detected in Brazil, and the resulting epidemic has spread throughout the Western Hemisphere. Severe complications from ZIKV infection include neurological disorders such as Guillain-Barré syndrome in adults and a variety of fetal abnormalities, including microcephaly, blindness, placental insufficiency, and fetal demise. There is an urgent need for tools and reagents to study the pathogenesis of epidemic ZIKV and for testing vaccines and antivirals. Using a reverse genetics platform, we generated six ZIKV infectious clones and derivative viruses representing diverse temporal and geographic origins. These include three versions of MR766, the prototype 1947 strain (with and without a glycosylation site in the envelope protein), and H/PF/2013, a 2013 human isolate from French Polynesia representative of the virus introduced to Brazil. In the course of synthesizing a clone of a circulating Brazilian strain, phylogenetic studies identified two distinct ZIKV clades in Brazil. We reconstructed viable clones of strains SPH2015 and BeH819015, representing ancestral members of each clade. We assessed recombinant virus replication, binding to monoclonal antibodies, and virulence in mice. This panel of molecular clones and recombinant virus isolates will enable targeted studies of viral determinants of pathogenesis, adaptation, and evolution, as well as the rational attenuation of contemporary outbreak strains to facilitate the design of vaccines and therapeutics. IMPORTANCE Viral emergence is a poorly understood process as evidenced by the sudden emergence of Zika virus in Latin America and the Caribbean. Malleable reagents that both predate and span an expanding epidemic are key to understanding the virologic determinants that regulate pathogenesis and transmission. We have generated representative cDNA molecular clones and recombinant viruses that span the known ZIKV family tree, including early Brazilian isolates. Recombinant viruses replicated efficiently in cell culture and were pathogenic in immunodeficient mice, providing a genetic platform for rational vaccine and therapeutic design.
format	article
author	Douglas G. Widman Ellen Young Boyd L. Yount Kenneth S. Plante Emily N. Gallichotte Derek L. Carbaugh Kayla M. Peck Jessica Plante Jesica Swanstrom Mark T. Heise Helen M. Lazear Ralph S. Baric
author_facet	Douglas G. Widman Ellen Young Boyd L. Yount Kenneth S. Plante Emily N. Gallichotte Derek L. Carbaugh Kayla M. Peck Jessica Plante Jesica Swanstrom Mark T. Heise Helen M. Lazear Ralph S. Baric
author_sort	Douglas G. Widman
title	A Reverse Genetics Platform That Spans the Zika Virus Family Tree
title_short	A Reverse Genetics Platform That Spans the Zika Virus Family Tree
title_full	A Reverse Genetics Platform That Spans the Zika Virus Family Tree
title_fullStr	A Reverse Genetics Platform That Spans the Zika Virus Family Tree
title_full_unstemmed	A Reverse Genetics Platform That Spans the Zika Virus Family Tree
title_sort	reverse genetics platform that spans the zika virus family tree
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/badd76acfe644f0ea9b1b6e9afa5d37b
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A Reverse Genetics Platform That Spans the Zika Virus Family Tree

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