The functional significance of microRNA-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.

<h4>Background</h4>The recurrence of colorectal cancer (CRC) is frequent within the first year of curative resection surgery and may be unavoidable. microRNAs have been suggested to play roles in carcinogenesis and cancer recurrence. We recently identified microRNA-29c (miRNA-29c) as a p...

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Autores principales:	I-Ping Yang, Hsiang-Lin Tsai, Ching-Wen Huang, Ming-Yii Huang, Ming-Feng Hou, Suh-Hang Hank Juo, Jaw-Yuan Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bae04e5843a34a948b04ba9a999de440
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spelling	oai:doaj.org-article:bae04e5843a34a948b04ba9a999de4402021-11-18T07:39:34ZThe functional significance of microRNA-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.1932-620310.1371/journal.pone.0066842https://doaj.org/article/bae04e5843a34a948b04ba9a999de4402013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23840538/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The recurrence of colorectal cancer (CRC) is frequent within the first year of curative resection surgery and may be unavoidable. microRNAs have been suggested to play roles in carcinogenesis and cancer recurrence. We recently identified microRNA-29c (miRNA-29c) as a predictor of early recurrence in CRC. In the present study, we further investigated the functions and serum level of miRNA-29c in relation to early recurrence of CRC.<h4>Methods</h4>First we further confirmed overexpression of miRNA-29c in non-early relapse subjects. Gain-of-function in vitro studies were used to evaluate the effect of miRNA-29c on cell proliferation, migration, invasion, and cell cycle progression. The colon cancer cell line Caco2 and a stable clone overexpressing miRNA-29c were xenografted to evaluate the in vivo effect of miRNA-29c in null mice. Finally, circulating miRNA-29c was investigated as a potential biomarker for identifying early relapse.<h4>Results</h4>miRNA-29c expression significantly decreased during early relapse compared to non-early relapse in UICC stage II and III CRC patients (P = 0.021). In vitro studies showed that overexpression of miRNA-29c inhibited cell proliferation and migration. The cell cycle studies also revealed that miRNA-29c caused an accumulation of the G1 and G2 population. In vivo, miRNA-29c suppressed tumor growth in null mice. The serum miRNA-29c increased significantly in early relapsed patients compared to non-early elapsed patients (P = 0.012).<h4>Conclusions</h4>miRNA-29c shows anti-tumorigenesis activity, and preoperative circulating miRNA-29c levels can be used to predict postoperative early relapse of CRC.I-Ping YangHsiang-Lin TsaiChing-Wen HuangMing-Yii HuangMing-Feng HouSuh-Hang Hank JuoJaw-Yuan WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66842 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q I-Ping Yang Hsiang-Lin Tsai Ching-Wen Huang Ming-Yii Huang Ming-Feng Hou Suh-Hang Hank Juo Jaw-Yuan Wang The functional significance of microRNA-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.
description	<h4>Background</h4>The recurrence of colorectal cancer (CRC) is frequent within the first year of curative resection surgery and may be unavoidable. microRNAs have been suggested to play roles in carcinogenesis and cancer recurrence. We recently identified microRNA-29c (miRNA-29c) as a predictor of early recurrence in CRC. In the present study, we further investigated the functions and serum level of miRNA-29c in relation to early recurrence of CRC.<h4>Methods</h4>First we further confirmed overexpression of miRNA-29c in non-early relapse subjects. Gain-of-function in vitro studies were used to evaluate the effect of miRNA-29c on cell proliferation, migration, invasion, and cell cycle progression. The colon cancer cell line Caco2 and a stable clone overexpressing miRNA-29c were xenografted to evaluate the in vivo effect of miRNA-29c in null mice. Finally, circulating miRNA-29c was investigated as a potential biomarker for identifying early relapse.<h4>Results</h4>miRNA-29c expression significantly decreased during early relapse compared to non-early relapse in UICC stage II and III CRC patients (P = 0.021). In vitro studies showed that overexpression of miRNA-29c inhibited cell proliferation and migration. The cell cycle studies also revealed that miRNA-29c caused an accumulation of the G1 and G2 population. In vivo, miRNA-29c suppressed tumor growth in null mice. The serum miRNA-29c increased significantly in early relapsed patients compared to non-early elapsed patients (P = 0.012).<h4>Conclusions</h4>miRNA-29c shows anti-tumorigenesis activity, and preoperative circulating miRNA-29c levels can be used to predict postoperative early relapse of CRC.
format	article
author	I-Ping Yang Hsiang-Lin Tsai Ching-Wen Huang Ming-Yii Huang Ming-Feng Hou Suh-Hang Hank Juo Jaw-Yuan Wang
author_facet	I-Ping Yang Hsiang-Lin Tsai Ching-Wen Huang Ming-Yii Huang Ming-Feng Hou Suh-Hang Hank Juo Jaw-Yuan Wang
author_sort	I-Ping Yang
title	The functional significance of microRNA-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.
title_short	The functional significance of microRNA-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.
title_full	The functional significance of microRNA-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.
title_fullStr	The functional significance of microRNA-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.
title_full_unstemmed	The functional significance of microRNA-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.
title_sort	functional significance of microrna-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/bae04e5843a34a948b04ba9a999de440
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The functional significance of microRNA-29c in patients with colorectal cancer: a potential circulating biomarker for predicting early relapse.

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