Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.

The aim of this study was to investigate the atomic composition and the proteome of the salivary proteins adsorbed on the surface of orthodontic metallic bracket. For this, the atomic composition of orthodontic metallic brackets was analyzed with X-ray Photoelectron Spectroscopy (XPS). The acquired...

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Autores principales:	Walter Luiz Siqueira, Maria Pia Canales, Karla Tonelli Bicalho Crosara, Lina Maria Marin, Yizhi Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bae5ae6e450c4a799d2c870ee2a937f5
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spelling	oai:doaj.org-article:bae5ae6e450c4a799d2c870ee2a937f52021-12-02T20:04:48ZProteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.1932-620310.1371/journal.pone.0254909https://doaj.org/article/bae5ae6e450c4a799d2c870ee2a937f52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254909https://doaj.org/toc/1932-6203The aim of this study was to investigate the atomic composition and the proteome of the salivary proteins adsorbed on the surface of orthodontic metallic bracket. For this, the atomic composition of orthodontic metallic brackets was analyzed with X-ray Photoelectron Spectroscopy (XPS). The acquired bracket pellicle was characterized after brackets were immersed in human whole saliva supernatant for 2 hours at 37°C. Hydroxyapatite (HA) discs were used as a control. Acquired pellicle was harvested from the HA discs (n = 12) and from the metallic brackets (n = 12). Proteomics based on mass spectrometry technology was used for salivary protein identification and characterization. Results showed that most of the proteins adsorbed on the surface of orthodontic metallic brackets and on the HA discs were identified specifically to each group, indicating a small overlapping between the salivary proteins on each study group. A total of 311 proteins present on the HA discs were unique to this group while 253 proteins were unique to metallic brackets, and only 45 proteins were common to the two groups. Even though most proteins were unique to each study group, proteins related to antimicrobial activity, lubrication, and remineralization were present in both groups. These findings demonstrate that the salivary proteins adsorbed on the bracket surface are dependent on the material molecular composition.Walter Luiz SiqueiraMaria Pia CanalesKarla Tonelli Bicalho CrosaraLina Maria MarinYizhi XiaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254909 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Walter Luiz Siqueira Maria Pia Canales Karla Tonelli Bicalho Crosara Lina Maria Marin Yizhi Xiao Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.
description	The aim of this study was to investigate the atomic composition and the proteome of the salivary proteins adsorbed on the surface of orthodontic metallic bracket. For this, the atomic composition of orthodontic metallic brackets was analyzed with X-ray Photoelectron Spectroscopy (XPS). The acquired bracket pellicle was characterized after brackets were immersed in human whole saliva supernatant for 2 hours at 37°C. Hydroxyapatite (HA) discs were used as a control. Acquired pellicle was harvested from the HA discs (n = 12) and from the metallic brackets (n = 12). Proteomics based on mass spectrometry technology was used for salivary protein identification and characterization. Results showed that most of the proteins adsorbed on the surface of orthodontic metallic brackets and on the HA discs were identified specifically to each group, indicating a small overlapping between the salivary proteins on each study group. A total of 311 proteins present on the HA discs were unique to this group while 253 proteins were unique to metallic brackets, and only 45 proteins were common to the two groups. Even though most proteins were unique to each study group, proteins related to antimicrobial activity, lubrication, and remineralization were present in both groups. These findings demonstrate that the salivary proteins adsorbed on the bracket surface are dependent on the material molecular composition.
format	article
author	Walter Luiz Siqueira Maria Pia Canales Karla Tonelli Bicalho Crosara Lina Maria Marin Yizhi Xiao
author_facet	Walter Luiz Siqueira Maria Pia Canales Karla Tonelli Bicalho Crosara Lina Maria Marin Yizhi Xiao
author_sort	Walter Luiz Siqueira
title	Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.
title_short	Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.
title_full	Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.
title_fullStr	Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.
title_full_unstemmed	Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.
title_sort	proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/bae5ae6e450c4a799d2c870ee2a937f5
work_keys_str_mv	AT walterluizsiqueira proteomedifferenceamongthesalivaryproteinsadsorbedontometallicorthodonticbracketsandhydroxyapatitediscs AT mariapiacanales proteomedifferenceamongthesalivaryproteinsadsorbedontometallicorthodonticbracketsandhydroxyapatitediscs AT karlatonellibicalhocrosara proteomedifferenceamongthesalivaryproteinsadsorbedontometallicorthodonticbracketsandhydroxyapatitediscs AT linamariamarin proteomedifferenceamongthesalivaryproteinsadsorbedontometallicorthodonticbracketsandhydroxyapatitediscs AT yizhixiao proteomedifferenceamongthesalivaryproteinsadsorbedontometallicorthodonticbracketsandhydroxyapatitediscs
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Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.

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