EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC

Synthesis of mRNAs for the key immunoactive cytokines was studied in mononuclear cells (MNC) from human peripheral blood, using reverse transcription and polymerase chain reaction, after incubation of isolated cells with human serum γ-globulin, zinc ions, or zinc-γ-globulin metal complexes. A consti...

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Autores principales:	S. B. Cheknev, M. V. Mezentseva, I. M. Shapoval, A. N. Narovlyansky
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	γ-globulin metal complexes zinc genes cytokines expression Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/bae762c3af454c1b9b9ba2347ae17386
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spelling	oai:doaj.org-article:bae762c3af454c1b9b9ba2347ae173862021-11-18T08:03:37ZEXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC1563-06252313-741X10.15789/1563-0625-2010-3-171-176https://doaj.org/article/bae762c3af454c1b9b9ba2347ae173862014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/64https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XSynthesis of mRNAs for the key immunoactive cytokines was studied in mononuclear cells (MNC) from human peripheral blood, using reverse transcription and polymerase chain reaction, after incubation of isolated cells with human serum γ-globulin, zinc ions, or zinc-γ-globulin metal complexes. A constitutive presence of interferon-α (IFNα) and interleukin-18 (IL-18) mRNA was shown in MNC of all of the subjects observed, whereas IFNγ, IL-4, or IL-8 mRNA synthesis was not detectable. Expression of IL-1β, IL-2, IL-6 и IL-10 was revealed in only a half of cases. It has been shown that pure γ-globulins did induce IFNγ gene expression and enhance transcriptional activity of IL-1β and IL-2 genes. Zinc cations did also promote transcription of IL-1β gene, along with reduced synthesis of IL-2 and IFNγ mRNA, compared to the γ-globulin. In presence of γ-globulin-zinc complexes, IL-1β mRNA was synthesized at the same level, as following induction with control γ-globulin, whereas IL-2 gene expression was reduced, and IFNγ mRNA synthesis was abolished. The results are discussed in terms of immune response polarization in presence of γ-globulins and their metal complexes with zinc.S. B. CheknevM. V. MezentsevaI. M. ShapovalA. N. NarovlyanskySPb RAACIarticleγ-globulinmetal complexeszincgenescytokinesexpressionImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 12, Iss 3, Pp 171-176 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	γ-globulin metal complexes zinc genes cytokines expression Immunologic diseases. Allergy RC581-607
spellingShingle	γ-globulin metal complexes zinc genes cytokines expression Immunologic diseases. Allergy RC581-607 S. B. Cheknev M. V. Mezentseva I. M. Shapoval A. N. Narovlyansky EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC
description	Synthesis of mRNAs for the key immunoactive cytokines was studied in mononuclear cells (MNC) from human peripheral blood, using reverse transcription and polymerase chain reaction, after incubation of isolated cells with human serum γ-globulin, zinc ions, or zinc-γ-globulin metal complexes. A constitutive presence of interferon-α (IFNα) and interleukin-18 (IL-18) mRNA was shown in MNC of all of the subjects observed, whereas IFNγ, IL-4, or IL-8 mRNA synthesis was not detectable. Expression of IL-1β, IL-2, IL-6 и IL-10 was revealed in only a half of cases. It has been shown that pure γ-globulins did induce IFNγ gene expression and enhance transcriptional activity of IL-1β and IL-2 genes. Zinc cations did also promote transcription of IL-1β gene, along with reduced synthesis of IL-2 and IFNγ mRNA, compared to the γ-globulin. In presence of γ-globulin-zinc complexes, IL-1β mRNA was synthesized at the same level, as following induction with control γ-globulin, whereas IL-2 gene expression was reduced, and IFNγ mRNA synthesis was abolished. The results are discussed in terms of immune response polarization in presence of γ-globulins and their metal complexes with zinc.
format	article
author	S. B. Cheknev M. V. Mezentseva I. M. Shapoval A. N. Narovlyansky
author_facet	S. B. Cheknev M. V. Mezentseva I. M. Shapoval A. N. Narovlyansky
author_sort	S. B. Cheknev
title	EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC
title_short	EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC
title_full	EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC
title_fullStr	EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC
title_full_unstemmed	EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC
title_sort	expression of the cytokine genes induced by human serum γ-globulin and by its metal complexes with zinc
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/bae762c3af454c1b9b9ba2347ae17386
work_keys_str_mv	AT sbcheknev expressionofthecytokinegenesinducedbyhumanserumgglobulinandbyitsmetalcomplexeswithzinc AT mvmezentseva expressionofthecytokinegenesinducedbyhumanserumgglobulinandbyitsmetalcomplexeswithzinc AT imshapoval expressionofthecytokinegenesinducedbyhumanserumgglobulinandbyitsmetalcomplexeswithzinc AT annarovlyansky expressionofthecytokinegenesinducedbyhumanserumgglobulinandbyitsmetalcomplexeswithzinc
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EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC

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