The amniotic fluid cell-free transcriptome in spontaneous preterm labor

Abstract The amniotic fluid (AF) cell-free RNA was shown to reflect physiological and pathological processes in pregnancy, but its value in the prediction of spontaneous preterm delivery is unknown. Herein we profiled cell-free RNA in AF samples collected from women who underwent transabdominal amni...

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Autores principales:	Gaurav Bhatti, Roberto Romero, Nardhy Gomez-Lopez, Roger Pique-Regi, Percy Pacora, Eunjung Jung, Lami Yeo, Chaur-Dong Hsu, Mahendra Kavdia, Adi L. Tarca
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/baebc80f2248497baa8f0fa17c083ace
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spelling	oai:doaj.org-article:baebc80f2248497baa8f0fa17c083ace2021-12-02T16:32:08ZThe amniotic fluid cell-free transcriptome in spontaneous preterm labor10.1038/s41598-021-92439-x2045-2322https://doaj.org/article/baebc80f2248497baa8f0fa17c083ace2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92439-xhttps://doaj.org/toc/2045-2322Abstract The amniotic fluid (AF) cell-free RNA was shown to reflect physiological and pathological processes in pregnancy, but its value in the prediction of spontaneous preterm delivery is unknown. Herein we profiled cell-free RNA in AF samples collected from women who underwent transabdominal amniocentesis after an episode of spontaneous preterm labor and subsequently delivered within 24 h (n = 10) or later (n = 28) in gestation. Expression of known placental single-cell RNA-Seq signatures was quantified in AF cell-free RNA and compared between the groups. Random forest models were applied to predict time-to-delivery after amniocentesis. There were 2385 genes differentially expressed in AF samples of women who delivered within 24 h of amniocentesis compared to gestational age-matched samples from women who delivered after 24 h of amniocentesis. Genes with cell-free RNA changes were associated with immune and inflammatory processes related to the onset of labor, and the expression of placental single-cell RNA-Seq signatures of immune cells was increased with imminent delivery. AF transcriptomic prediction models captured these effects and predicted delivery within 24 h of amniocentesis (AUROC = 0.81). These results may inform the development of biomarkers for spontaneous preterm birth.Gaurav BhattiRoberto RomeroNardhy Gomez-LopezRoger Pique-RegiPercy PacoraEunjung JungLami YeoChaur-Dong HsuMahendra KavdiaAdi L. TarcaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gaurav Bhatti Roberto Romero Nardhy Gomez-Lopez Roger Pique-Regi Percy Pacora Eunjung Jung Lami Yeo Chaur-Dong Hsu Mahendra Kavdia Adi L. Tarca The amniotic fluid cell-free transcriptome in spontaneous preterm labor
description	Abstract The amniotic fluid (AF) cell-free RNA was shown to reflect physiological and pathological processes in pregnancy, but its value in the prediction of spontaneous preterm delivery is unknown. Herein we profiled cell-free RNA in AF samples collected from women who underwent transabdominal amniocentesis after an episode of spontaneous preterm labor and subsequently delivered within 24 h (n = 10) or later (n = 28) in gestation. Expression of known placental single-cell RNA-Seq signatures was quantified in AF cell-free RNA and compared between the groups. Random forest models were applied to predict time-to-delivery after amniocentesis. There were 2385 genes differentially expressed in AF samples of women who delivered within 24 h of amniocentesis compared to gestational age-matched samples from women who delivered after 24 h of amniocentesis. Genes with cell-free RNA changes were associated with immune and inflammatory processes related to the onset of labor, and the expression of placental single-cell RNA-Seq signatures of immune cells was increased with imminent delivery. AF transcriptomic prediction models captured these effects and predicted delivery within 24 h of amniocentesis (AUROC = 0.81). These results may inform the development of biomarkers for spontaneous preterm birth.
format	article
author	Gaurav Bhatti Roberto Romero Nardhy Gomez-Lopez Roger Pique-Regi Percy Pacora Eunjung Jung Lami Yeo Chaur-Dong Hsu Mahendra Kavdia Adi L. Tarca
author_facet	Gaurav Bhatti Roberto Romero Nardhy Gomez-Lopez Roger Pique-Regi Percy Pacora Eunjung Jung Lami Yeo Chaur-Dong Hsu Mahendra Kavdia Adi L. Tarca
author_sort	Gaurav Bhatti
title	The amniotic fluid cell-free transcriptome in spontaneous preterm labor
title_short	The amniotic fluid cell-free transcriptome in spontaneous preterm labor
title_full	The amniotic fluid cell-free transcriptome in spontaneous preterm labor
title_fullStr	The amniotic fluid cell-free transcriptome in spontaneous preterm labor
title_full_unstemmed	The amniotic fluid cell-free transcriptome in spontaneous preterm labor
title_sort	amniotic fluid cell-free transcriptome in spontaneous preterm labor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/baebc80f2248497baa8f0fa17c083ace
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The amniotic fluid cell-free transcriptome in spontaneous preterm labor

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