A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec

ABSTRACT Neisseria meningitidis carriage data are necessary to inform serogroup B (NmB) immunization program implementation. This longitudinal study compared detection methods to measure N. meningitidis throat carriage prevalence in Quebec from November 2010 to December 2013 using cultured swab isol...

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Autores principales:	Rodica Gilca, Philippe De Wals, Sheila M. Nolan, Nicholas Kitchin, Joseph J. Eiden, Qin Jiang, C. Hal Jones, Kathrin U. Jansen, Annaliesa S. Anderson, Louise Pedneault
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Neisseria meningitidis NmB epidemiology factor H binding protein oropharyngeal carriage serogroup B Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/baed79086a814f6b96fec02338df9094
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spelling	oai:doaj.org-article:baed79086a814f6b96fec02338df90942021-11-15T15:22:21ZA Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec10.1128/mSphere.00427-182379-5042https://doaj.org/article/baed79086a814f6b96fec02338df90942018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00427-18https://doaj.org/toc/2379-5042ABSTRACT Neisseria meningitidis carriage data are necessary to inform serogroup B (NmB) immunization program implementation. This longitudinal study compared detection methods to measure N. meningitidis throat carriage prevalence in Quebec from November 2010 to December 2013 using cultured swab isolates and direct swab PCR from students in ninth grade (aged 13 to 15 years; n = 534) and eleventh grade/college entry (16 to 18 years; n = 363) and in university students in dormitories (18 to 25 years; n = 360) at 3 time points per group. Meningococcal and NmB carriage rates were lower in ninth- and eleventh-grade/college entry students than university students, regardless of methodology. Genotyping cultured isolates by PCR detected NmB and non-NmB in 2.1% and 7.3% of ninth-grade students, in 1.7% and 7.2% of eleventh-grade/college entry students, and in 7.5% and 21.9% of university students, respectively. NmB acquisition rates were 1.9, 0.7, and 3.3 per 1,000 person-months across respective age groups. Most NmB isolates (94.7%, 76.9%, and 86.8%, respectively) expressed subfamily A factor H binding-protein (fHBP) variants. The most common non-NmB serogroups were NmY (1.7%/1.1%) from ninth grade and eleventh grade/college entry and NmW (2.8%) from university students. Genomic analyses detected disease-associated sequence types in carriage isolates, and carriage could persist for months. This is the largest longitudinal carriage study in Canada and the first to report fHBP variants in NmB carriage isolates in healthy Canadians. These data contribute to identification of the optimal window for NmB vaccination in precollege adolescents and provide a baseline for investigating NmB vaccination effects on carriage in this population. IMPORTANCE Disease caused by Neisseria meningitidis is associated with serious complications and a high fatality rate. Asymptomatic individuals can harbor the bacterium in the throat, a state known as “carriage,” which can lead to person-to-person spread of the pathogen. This study examined N. meningitidis carriage from 2010 to 2013 among 2 groups in the Quebec City region: ninth-grade students (aged 13 to 15 years), who were also followed in their last year of high school (eleventh grade/college entry; 16 to 18 years), and university students (18 to 25 years); both groups have been shown in some other geographic regions to have high rates of carriage. This study demonstrated that N. meningitidis carriage rates were higher among university students in dormitories than ninth-grade and eleventh-grade/college entry students. Understanding carriage rates in these age groups leads to better strategies to control N. meningitidis by targeting vaccination to those responsible for transmission within the population.Rodica GilcaPhilippe De WalsSheila M. NolanNicholas KitchinJoseph J. EidenQin JiangC. Hal JonesKathrin U. JansenAnnaliesa S. AndersonLouise PedneaultAmerican Society for MicrobiologyarticleNeisseria meningitidisNmBepidemiologyfactor H binding proteinoropharyngeal carriageserogroup BMicrobiologyQR1-502ENmSphere, Vol 3, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neisseria meningitidis NmB epidemiology factor H binding protein oropharyngeal carriage serogroup B Microbiology QR1-502
spellingShingle	Neisseria meningitidis NmB epidemiology factor H binding protein oropharyngeal carriage serogroup B Microbiology QR1-502 Rodica Gilca Philippe De Wals Sheila M. Nolan Nicholas Kitchin Joseph J. Eiden Qin Jiang C. Hal Jones Kathrin U. Jansen Annaliesa S. Anderson Louise Pedneault A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec
description	ABSTRACT Neisseria meningitidis carriage data are necessary to inform serogroup B (NmB) immunization program implementation. This longitudinal study compared detection methods to measure N. meningitidis throat carriage prevalence in Quebec from November 2010 to December 2013 using cultured swab isolates and direct swab PCR from students in ninth grade (aged 13 to 15 years; n = 534) and eleventh grade/college entry (16 to 18 years; n = 363) and in university students in dormitories (18 to 25 years; n = 360) at 3 time points per group. Meningococcal and NmB carriage rates were lower in ninth- and eleventh-grade/college entry students than university students, regardless of methodology. Genotyping cultured isolates by PCR detected NmB and non-NmB in 2.1% and 7.3% of ninth-grade students, in 1.7% and 7.2% of eleventh-grade/college entry students, and in 7.5% and 21.9% of university students, respectively. NmB acquisition rates were 1.9, 0.7, and 3.3 per 1,000 person-months across respective age groups. Most NmB isolates (94.7%, 76.9%, and 86.8%, respectively) expressed subfamily A factor H binding-protein (fHBP) variants. The most common non-NmB serogroups were NmY (1.7%/1.1%) from ninth grade and eleventh grade/college entry and NmW (2.8%) from university students. Genomic analyses detected disease-associated sequence types in carriage isolates, and carriage could persist for months. This is the largest longitudinal carriage study in Canada and the first to report fHBP variants in NmB carriage isolates in healthy Canadians. These data contribute to identification of the optimal window for NmB vaccination in precollege adolescents and provide a baseline for investigating NmB vaccination effects on carriage in this population. IMPORTANCE Disease caused by Neisseria meningitidis is associated with serious complications and a high fatality rate. Asymptomatic individuals can harbor the bacterium in the throat, a state known as “carriage,” which can lead to person-to-person spread of the pathogen. This study examined N. meningitidis carriage from 2010 to 2013 among 2 groups in the Quebec City region: ninth-grade students (aged 13 to 15 years), who were also followed in their last year of high school (eleventh grade/college entry; 16 to 18 years), and university students (18 to 25 years); both groups have been shown in some other geographic regions to have high rates of carriage. This study demonstrated that N. meningitidis carriage rates were higher among university students in dormitories than ninth-grade and eleventh-grade/college entry students. Understanding carriage rates in these age groups leads to better strategies to control N. meningitidis by targeting vaccination to those responsible for transmission within the population.
format	article
author	Rodica Gilca Philippe De Wals Sheila M. Nolan Nicholas Kitchin Joseph J. Eiden Qin Jiang C. Hal Jones Kathrin U. Jansen Annaliesa S. Anderson Louise Pedneault
author_facet	Rodica Gilca Philippe De Wals Sheila M. Nolan Nicholas Kitchin Joseph J. Eiden Qin Jiang C. Hal Jones Kathrin U. Jansen Annaliesa S. Anderson Louise Pedneault
author_sort	Rodica Gilca
title	A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec
title_short	A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec
title_full	A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec
title_fullStr	A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec
title_full_unstemmed	A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec
title_sort	longitudinal epidemiology study of meningococcal carriage in students 13 to 25 years old in quebec
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/baed79086a814f6b96fec02338df9094
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A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec

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