Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats

Saud Alarifi,1 Daoud Ali,1 Amin A Al-Doaiss,1,2 Bahy A Ali,1,3 Mukhtar Ahmed,1 Abdulaziz A Al-Khedhairy11Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Anatomy and Histology Department, Faculty of Medicine, Sana'a University, Sana'a, Republic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alarifi S, Ali D, Al-Doaiss AA, Ali BA, Ahmed M, Al-Khedhairy AA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/baf46c9a90bd4d23be037a085916da73
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:baf46c9a90bd4d23be037a085916da73
record_format	dspace
spelling	oai:doaj.org-article:baf46c9a90bd4d23be037a085916da732021-12-02T09:08:48ZHistologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats1176-91141178-2013https://doaj.org/article/baf46c9a90bd4d23be037a085916da732013-10-01T00:00:00Zhttp://www.dovepress.com/histologic-and-apoptotic-changes-induced-by-titanium-dioxide-nanoparti-a14680https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Saud Alarifi,1 Daoud Ali,1 Amin A Al-Doaiss,1,2 Bahy A Ali,1,3 Mukhtar Ahmed,1 Abdulaziz A Al-Khedhairy11Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Anatomy and Histology Department, Faculty of Medicine, Sana'a University, Sana'a, Republic of Yemen; 3Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Alexandria, EgyptAbstract: Titanium dioxide (TiO2) nanoparticles are among the top five nanoparticles used in consumer products, paints, and pharmaceutical preparations. Given that exposure to such nanoparticles is mainly via the skin and inhalation, the present study was conducted in male Wistar albino rats (Rattus norvegicus). Our aim was to investigate the effect of TiO2 nanoparticles on hepatic tissue in an attempt to understand their toxicity and the potential effect of their therapeutic and diagnostic use. To investigate the effects of TiO2 nanoparticles on liver tissue, 30 healthy male Wistar albino rats were exposed to TiO2 nanoparticles at doses of 63 mg, 126 mg, and 252 mg per animal for 24 and 48 hours. Serum glutamate oxaloacetate transaminase and alkaline phosphatase activity was altered. Changes in hepatocytes can be summarized as hydropic degeneration, cloudy swelling, fatty degeneration, portal and lobular infiltration by chronic inflammatory cells, and congested dilated central veins. The histologic alterations observed might be an indication of hepatocyte injury due to the toxicity of TiO2 nanoparticles, resulting in an inability to deal with accumulated residues from the metabolic and structural disturbances caused by these nanoparticles. The appearance of cytoplasmic degeneration and destruction of nuclei in hepatocytes suggests that TiO2 nanoparticles interact with proteins and enzymes in hepatic tissue, interfering with antioxidant defense mechanisms and leading to generation of reactive oxygen species which, in turn, may induce stress in hepatocytes, promoting atrophy, apoptosis, and necrosis. More immunohistochemical and ultrastructural investigations are needed in relation to TiO2 nanoparticles and their potential effects when used as therapeutic and diagnostic tools.Keywords: TiO2 nanoparticles, rats, liver, histology, TUNEL assay, alkaline phosphatase, apoptosisAlarifi SAli DAl-Doaiss AAAli BAAhmed MAl-Khedhairy AADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 3937-3943 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Alarifi S Ali D Al-Doaiss AA Ali BA Ahmed M Al-Khedhairy AA Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats
description	Saud Alarifi,1 Daoud Ali,1 Amin A Al-Doaiss,1,2 Bahy A Ali,1,3 Mukhtar Ahmed,1 Abdulaziz A Al-Khedhairy11Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Anatomy and Histology Department, Faculty of Medicine, Sana'a University, Sana'a, Republic of Yemen; 3Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Alexandria, EgyptAbstract: Titanium dioxide (TiO2) nanoparticles are among the top five nanoparticles used in consumer products, paints, and pharmaceutical preparations. Given that exposure to such nanoparticles is mainly via the skin and inhalation, the present study was conducted in male Wistar albino rats (Rattus norvegicus). Our aim was to investigate the effect of TiO2 nanoparticles on hepatic tissue in an attempt to understand their toxicity and the potential effect of their therapeutic and diagnostic use. To investigate the effects of TiO2 nanoparticles on liver tissue, 30 healthy male Wistar albino rats were exposed to TiO2 nanoparticles at doses of 63 mg, 126 mg, and 252 mg per animal for 24 and 48 hours. Serum glutamate oxaloacetate transaminase and alkaline phosphatase activity was altered. Changes in hepatocytes can be summarized as hydropic degeneration, cloudy swelling, fatty degeneration, portal and lobular infiltration by chronic inflammatory cells, and congested dilated central veins. The histologic alterations observed might be an indication of hepatocyte injury due to the toxicity of TiO2 nanoparticles, resulting in an inability to deal with accumulated residues from the metabolic and structural disturbances caused by these nanoparticles. The appearance of cytoplasmic degeneration and destruction of nuclei in hepatocytes suggests that TiO2 nanoparticles interact with proteins and enzymes in hepatic tissue, interfering with antioxidant defense mechanisms and leading to generation of reactive oxygen species which, in turn, may induce stress in hepatocytes, promoting atrophy, apoptosis, and necrosis. More immunohistochemical and ultrastructural investigations are needed in relation to TiO2 nanoparticles and their potential effects when used as therapeutic and diagnostic tools.Keywords: TiO2 nanoparticles, rats, liver, histology, TUNEL assay, alkaline phosphatase, apoptosis
format	article
author	Alarifi S Ali D Al-Doaiss AA Ali BA Ahmed M Al-Khedhairy AA
author_facet	Alarifi S Ali D Al-Doaiss AA Ali BA Ahmed M Al-Khedhairy AA
author_sort	Alarifi S
title	Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats
title_short	Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats
title_full	Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats
title_fullStr	Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats
title_full_unstemmed	Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats
title_sort	histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/baf46c9a90bd4d23be037a085916da73
work_keys_str_mv	AT alarifis histologicandapoptoticchangesinducedbytitaniumdioxidenanoparticlesintheliversofrats AT alid histologicandapoptoticchangesinducedbytitaniumdioxidenanoparticlesintheliversofrats AT aldoaissaa histologicandapoptoticchangesinducedbytitaniumdioxidenanoparticlesintheliversofrats AT aliba histologicandapoptoticchangesinducedbytitaniumdioxidenanoparticlesintheliversofrats AT ahmedm histologicandapoptoticchangesinducedbytitaniumdioxidenanoparticlesintheliversofrats AT alkhedhairyaa histologicandapoptoticchangesinducedbytitaniumdioxidenanoparticlesintheliversofrats
_version_	1718398266271334400

Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats

Ejemplares similares