Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research

Abstract The yeast Hsp104 protein disaggregase is often used as a reporter for misfolded or damaged protein aggregates and protein quality control and ageing research. Observing Hsp104 fusions with fluorescent proteins is a popular approach to follow post stress protein aggregation, inclusion format...

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Autores principales:	Kara L. Schneider, Adam J. M. Wollman, Thomas Nyström, Sviatlana Shashkova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bafaf605c2364b1a9782e6ff88eb937a
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spelling	oai:doaj.org-article:bafaf605c2364b1a9782e6ff88eb937a2021-12-02T17:41:28ZComparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research10.1038/s41598-021-92249-12045-2322https://doaj.org/article/bafaf605c2364b1a9782e6ff88eb937a2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92249-1https://doaj.org/toc/2045-2322Abstract The yeast Hsp104 protein disaggregase is often used as a reporter for misfolded or damaged protein aggregates and protein quality control and ageing research. Observing Hsp104 fusions with fluorescent proteins is a popular approach to follow post stress protein aggregation, inclusion formation and disaggregation. While concerns that bigger protein tags, such as genetically encoded fluorescent tags, may affect protein behaviour and function have been around for quite some time, experimental evidence of how exactly the physiology of the protein of interest is altered within fluorescent protein fusions remains limited. To address this issue, we performed a comparative assessment of endogenously expressed Hsp104 fluorescent fusions function and behaviour. We provide experimental evidence that molecular behaviour may not only be altered by introducing a fluorescent protein tag but also varies depending on such a tag within the fusion. Although our findings are especially applicable to protein quality control and ageing research in yeast, similar effects may play a role in other eukaryotic systems.Kara L. SchneiderAdam J. M. WollmanThomas NyströmSviatlana ShashkovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kara L. Schneider Adam J. M. Wollman Thomas Nyström Sviatlana Shashkova Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research
description	Abstract The yeast Hsp104 protein disaggregase is often used as a reporter for misfolded or damaged protein aggregates and protein quality control and ageing research. Observing Hsp104 fusions with fluorescent proteins is a popular approach to follow post stress protein aggregation, inclusion formation and disaggregation. While concerns that bigger protein tags, such as genetically encoded fluorescent tags, may affect protein behaviour and function have been around for quite some time, experimental evidence of how exactly the physiology of the protein of interest is altered within fluorescent protein fusions remains limited. To address this issue, we performed a comparative assessment of endogenously expressed Hsp104 fluorescent fusions function and behaviour. We provide experimental evidence that molecular behaviour may not only be altered by introducing a fluorescent protein tag but also varies depending on such a tag within the fusion. Although our findings are especially applicable to protein quality control and ageing research in yeast, similar effects may play a role in other eukaryotic systems.
format	article
author	Kara L. Schneider Adam J. M. Wollman Thomas Nyström Sviatlana Shashkova
author_facet	Kara L. Schneider Adam J. M. Wollman Thomas Nyström Sviatlana Shashkova
author_sort	Kara L. Schneider
title	Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research
title_short	Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research
title_full	Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research
title_fullStr	Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research
title_full_unstemmed	Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research
title_sort	comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bafaf605c2364b1a9782e6ff88eb937a
work_keys_str_mv	AT karalschneider comparisonofendogenouslyexpressedfluorescentproteinfusionsbehaviourforproteinqualitycontrolandcellularageingresearch AT adamjmwollman comparisonofendogenouslyexpressedfluorescentproteinfusionsbehaviourforproteinqualitycontrolandcellularageingresearch AT thomasnystrom comparisonofendogenouslyexpressedfluorescentproteinfusionsbehaviourforproteinqualitycontrolandcellularageingresearch AT sviatlanashashkova comparisonofendogenouslyexpressedfluorescentproteinfusionsbehaviourforproteinqualitycontrolandcellularageingresearch
_version_	1718379665160142848

Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research

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