<italic toggle="yes">vanG</italic> Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to <named-content content-type="genus-species">Streptococcus agalactiae</named-content> and <named-content content-type="genus-species">Streptococcus anginosus</named-content>

ABSTRACT Three vancomycin-resistant streptococcal strains carrying vanG elements (two invasive Streptococcus agalactiae isolates [GBS-NY and GBS-NM, both serotype II and multilocus sequence type 22] and one Streptococcus anginosus [Sa]) were examined. The 45,585-bp elements found within Sa and GBS-N...

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Autores principales:	Velusamy Srinivasan, Benjamin J. Metcalf, Kristen M. Knipe, Mahamoudou Ouattara, Lesley McGee, Patricia L. Shewmaker, Anita Glennen, Megin Nichols, Carol Harris, Mary Brimmage, Belinda Ostrowsky, Connie J. Park, Stephanie J. Schrag, Michael A. Frace, Scott A. Sammons, Bernard Beall
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bafc82a0137b43feb85ad7638f6492ee
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spelling	oai:doaj.org-article:bafc82a0137b43feb85ad7638f6492ee2021-11-15T15:47:22Z<italic toggle="yes">vanG</italic> Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to <named-content content-type="genus-species">Streptococcus agalactiae</named-content> and <named-content content-type="genus-species">Streptococcus anginosus</named-content>10.1128/mBio.01386-142150-7511https://doaj.org/article/bafc82a0137b43feb85ad7638f6492ee2014-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01386-14https://doaj.org/toc/2150-7511ABSTRACT Three vancomycin-resistant streptococcal strains carrying vanG elements (two invasive Streptococcus agalactiae isolates [GBS-NY and GBS-NM, both serotype II and multilocus sequence type 22] and one Streptococcus anginosus [Sa]) were examined. The 45,585-bp elements found within Sa and GBS-NY were nearly identical (together designated vanG-1) and shared near-identity over an ~15-kb overlap with a previously described vanG element from Enterococcus faecalis. Unexpectedly, vanG-1 shared much less homology with the 49,321-bp vanG-2 element from GBS-NM, with widely different levels (50% to 99%) of sequence identity shared among 44 related open reading frames. Immediately adjacent to both vanG-1 and vanG-2 were 44,670-bp and 44,680-bp integrative conjugative element (ICE)-like sequences, designated ICE-r, that were nearly identical in the two group B streptococcal (GBS) strains. The dual vanG and ICE-r elements from both GBS strains were inserted at the same position, between bases 1328 and 1329, within the identical RNA methyltransferase (rumA) genes. A GenBank search revealed that although most GBS strains contained insertions within this specific site, only sequence type 22 (ST22) GBS strains contained highly related ICE-r derivatives. The vanG-1 element in Sa was also inserted within this position corresponding to its rumA homolog adjacent to an ICE-r derivative. vanG-1 insertions were previously reported within the same relative position in the E. faecalis rumA homolog. An ICE-r sequence perfectly conserved with respect to its counterpart in GBS-NY was apparent within the same site of the rumA homolog of a Streptococcus dysgalactiae subsp. equisimilis strain. Additionally, homologous vanG-like elements within the conserved rumA target site were evident in Roseburia intestinalis. IMPORTANCE These three streptococcal strains represent the first known vancomycin-resistant strains of their species. The collective observations made from these strains reveal a specific hot spot for insertional elements that is conserved between streptococci and different Gram-positive species. The two GBS strains potentially represent a GBS lineage that is predisposed to insertion of vanG elements.Velusamy SrinivasanBenjamin J. MetcalfKristen M. KnipeMahamoudou OuattaraLesley McGeePatricia L. ShewmakerAnita GlennenMegin NicholsCarol HarrisMary BrimmageBelinda OstrowskyConnie J. ParkStephanie J. SchragMichael A. FraceScott A. SammonsBernard BeallAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 4 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Velusamy Srinivasan Benjamin J. Metcalf Kristen M. Knipe Mahamoudou Ouattara Lesley McGee Patricia L. Shewmaker Anita Glennen Megin Nichols Carol Harris Mary Brimmage Belinda Ostrowsky Connie J. Park Stephanie J. Schrag Michael A. Frace Scott A. Sammons Bernard Beall <italic toggle="yes">vanG</italic> Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to <named-content content-type="genus-species">Streptococcus agalactiae</named-content> and <named-content content-type="genus-species">Streptococcus anginosus</named-content>
description	ABSTRACT Three vancomycin-resistant streptococcal strains carrying vanG elements (two invasive Streptococcus agalactiae isolates [GBS-NY and GBS-NM, both serotype II and multilocus sequence type 22] and one Streptococcus anginosus [Sa]) were examined. The 45,585-bp elements found within Sa and GBS-NY were nearly identical (together designated vanG-1) and shared near-identity over an ~15-kb overlap with a previously described vanG element from Enterococcus faecalis. Unexpectedly, vanG-1 shared much less homology with the 49,321-bp vanG-2 element from GBS-NM, with widely different levels (50% to 99%) of sequence identity shared among 44 related open reading frames. Immediately adjacent to both vanG-1 and vanG-2 were 44,670-bp and 44,680-bp integrative conjugative element (ICE)-like sequences, designated ICE-r, that were nearly identical in the two group B streptococcal (GBS) strains. The dual vanG and ICE-r elements from both GBS strains were inserted at the same position, between bases 1328 and 1329, within the identical RNA methyltransferase (rumA) genes. A GenBank search revealed that although most GBS strains contained insertions within this specific site, only sequence type 22 (ST22) GBS strains contained highly related ICE-r derivatives. The vanG-1 element in Sa was also inserted within this position corresponding to its rumA homolog adjacent to an ICE-r derivative. vanG-1 insertions were previously reported within the same relative position in the E. faecalis rumA homolog. An ICE-r sequence perfectly conserved with respect to its counterpart in GBS-NY was apparent within the same site of the rumA homolog of a Streptococcus dysgalactiae subsp. equisimilis strain. Additionally, homologous vanG-like elements within the conserved rumA target site were evident in Roseburia intestinalis. IMPORTANCE These three streptococcal strains represent the first known vancomycin-resistant strains of their species. The collective observations made from these strains reveal a specific hot spot for insertional elements that is conserved between streptococci and different Gram-positive species. The two GBS strains potentially represent a GBS lineage that is predisposed to insertion of vanG elements.
format	article
author	Velusamy Srinivasan Benjamin J. Metcalf Kristen M. Knipe Mahamoudou Ouattara Lesley McGee Patricia L. Shewmaker Anita Glennen Megin Nichols Carol Harris Mary Brimmage Belinda Ostrowsky Connie J. Park Stephanie J. Schrag Michael A. Frace Scott A. Sammons Bernard Beall
author_facet	Velusamy Srinivasan Benjamin J. Metcalf Kristen M. Knipe Mahamoudou Ouattara Lesley McGee Patricia L. Shewmaker Anita Glennen Megin Nichols Carol Harris Mary Brimmage Belinda Ostrowsky Connie J. Park Stephanie J. Schrag Michael A. Frace Scott A. Sammons Bernard Beall
author_sort	Velusamy Srinivasan
title	<italic toggle="yes">vanG</italic> Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to <named-content content-type="genus-species">Streptococcus agalactiae</named-content> and <named-content content-type="genus-species">Streptococcus anginosus</named-content>
title_short	<italic toggle="yes">vanG</italic> Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to <named-content content-type="genus-species">Streptococcus agalactiae</named-content> and <named-content content-type="genus-species">Streptococcus anginosus</named-content>
title_full	<italic toggle="yes">vanG</italic> Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to <named-content content-type="genus-species">Streptococcus agalactiae</named-content> and <named-content content-type="genus-species">Streptococcus anginosus</named-content>
title_fullStr	<italic toggle="yes">vanG</italic> Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to <named-content content-type="genus-species">Streptococcus agalactiae</named-content> and <named-content content-type="genus-species">Streptococcus anginosus</named-content>
title_full_unstemmed	<italic toggle="yes">vanG</italic> Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to <named-content content-type="genus-species">Streptococcus agalactiae</named-content> and <named-content content-type="genus-species">Streptococcus anginosus</named-content>
title_sort	<italic toggle="yes">vang</italic> element insertions within a conserved chromosomal site conferring vancomycin resistance to <named-content content-type="genus-species">streptococcus agalactiae</named-content> and <named-content content-type="genus-species">streptococcus anginosus</named-content>
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/bafc82a0137b43feb85ad7638f6492ee
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<italic toggle="yes">vanG</italic> Element Insertions within a Conserved Chromosomal Site Conferring Vancomycin Resistance to <named-content content-type="genus-species">Streptococcus agalactiae</named-content> and <named-content content-type="genus-species">Streptococcus anginosus</named-content>

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