Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration

Abstract Mitochondrial function declines with age and in some diseases, but we have been unable to analyze this in vivo. Here, we optically examine retinal mitochondrial function as well as choroidal oxygenation and hemodynamics in aging C57 and complement factor H (CFH−/−) mice, proposed models of...

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Autores principales:	Pardis Kaynezhad, Ilias Tachtsidis, Asmaa Aboelnour, Sobha Sivaprasad, Glen Jeffery
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bafd7149bf074acab8a4eb268d5549a8
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spelling	oai:doaj.org-article:bafd7149bf074acab8a4eb268d5549a82021-12-02T14:11:32ZWatching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration10.1038/s41598-021-82811-22045-2322https://doaj.org/article/bafd7149bf074acab8a4eb268d5549a82021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82811-2https://doaj.org/toc/2045-2322Abstract Mitochondrial function declines with age and in some diseases, but we have been unable to analyze this in vivo. Here, we optically examine retinal mitochondrial function as well as choroidal oxygenation and hemodynamics in aging C57 and complement factor H (CFH−/−) mice, proposed models of macular degeneration which suffer early retinal mitochondrial decline. In young C57s mitochondrial populations respire in coupled oscillatory behavior in cycles of ~ 8 min, which is phase linked to choroidal oscillatory hemodynamics. In aging C57s, the oscillations are less regular being ~ 14 min and more dissociated from choroidal hemodynamics. The mitochondrial oscillatory cycles are extended in CFH−/− mice being ~ 16 min and are further dissociated from choroidal hemodynamics. Mitochondrial decline occurs before age-related changes to choroidal vasculature, hence, is the likely origin of oscillatory disruption in hemodynamics. This technology offers a non-invasive technique to detect early retinal disease and its relationship to blood oxygenation in vivo and in real time.Pardis KaynezhadIlias TachtsidisAsmaa AboelnourSobha SivaprasadGlen JefferyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pardis Kaynezhad Ilias Tachtsidis Asmaa Aboelnour Sobha Sivaprasad Glen Jeffery Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration
description	Abstract Mitochondrial function declines with age and in some diseases, but we have been unable to analyze this in vivo. Here, we optically examine retinal mitochondrial function as well as choroidal oxygenation and hemodynamics in aging C57 and complement factor H (CFH−/−) mice, proposed models of macular degeneration which suffer early retinal mitochondrial decline. In young C57s mitochondrial populations respire in coupled oscillatory behavior in cycles of ~ 8 min, which is phase linked to choroidal oscillatory hemodynamics. In aging C57s, the oscillations are less regular being ~ 14 min and more dissociated from choroidal hemodynamics. The mitochondrial oscillatory cycles are extended in CFH−/− mice being ~ 16 min and are further dissociated from choroidal hemodynamics. Mitochondrial decline occurs before age-related changes to choroidal vasculature, hence, is the likely origin of oscillatory disruption in hemodynamics. This technology offers a non-invasive technique to detect early retinal disease and its relationship to blood oxygenation in vivo and in real time.
format	article
author	Pardis Kaynezhad Ilias Tachtsidis Asmaa Aboelnour Sobha Sivaprasad Glen Jeffery
author_facet	Pardis Kaynezhad Ilias Tachtsidis Asmaa Aboelnour Sobha Sivaprasad Glen Jeffery
author_sort	Pardis Kaynezhad
title	Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration
title_short	Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration
title_full	Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration
title_fullStr	Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration
title_full_unstemmed	Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration
title_sort	watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bafd7149bf074acab8a4eb268d5549a8
work_keys_str_mv	AT pardiskaynezhad watchingsynchronousmitochondrialrespirationintheretinaanditsinstabilityinamousemodelofmaculardegeneration AT iliastachtsidis watchingsynchronousmitochondrialrespirationintheretinaanditsinstabilityinamousemodelofmaculardegeneration AT asmaaaboelnour watchingsynchronousmitochondrialrespirationintheretinaanditsinstabilityinamousemodelofmaculardegeneration AT sobhasivaprasad watchingsynchronousmitochondrialrespirationintheretinaanditsinstabilityinamousemodelofmaculardegeneration AT glenjeffery watchingsynchronousmitochondrialrespirationintheretinaanditsinstabilityinamousemodelofmaculardegeneration
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Watching synchronous mitochondrial respiration in the retina and its instability in a mouse model of macular degeneration

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