Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice

For decades, numerous chemical pollutants have been described to interfere with endogenous hormone metabolism/signaling altering reproductive functions. Among these endocrine disrupting substances, Bisphenol A (BPA), a widely used compound, is known to negatively impact germ and somatic cells in the...

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Autores principales:	Margaux Wieckowski, Stéphanie Ranga, Delphine Moison, Sébastien Messiaen, Sonia Abdallah, Sylvie Granon, René Habert, Virginie Rouiller-Fabre, Gabriel Livera, Marie-Justine Guerquin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	endocrine disruptors reproduction germ cells Leydig cells γ-ray Bisphenol A Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/bafdb47791c44049ab2d5a94b133ba09
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spelling	oai:doaj.org-article:bafdb47791c44049ab2d5a94b133ba092021-11-11T17:15:04ZUnexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice10.3390/ijms2221118081422-00671661-6596https://doaj.org/article/bafdb47791c44049ab2d5a94b133ba092021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11808https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067For decades, numerous chemical pollutants have been described to interfere with endogenous hormone metabolism/signaling altering reproductive functions. Among these endocrine disrupting substances, Bisphenol A (BPA), a widely used compound, is known to negatively impact germ and somatic cells in the testis. Physical agents, such as ionizing radiation, were also described to perturb spermatogenesis. Despite the fact that we are constantly exposed to numerous environmental chemical and physical compounds, very few studies explore the impact of combined exposure to chemical and physical pollutants on reproductive health. The aim of this study was to describe the impact of fetal co-exposure to BPA and IR on testicular function in mice. We exposed pregnant mice to 10 µM BPA (corresponding to 0.5 mg/kg/day) in drinking water from 10.5 dpc until birth, and we irradiated mice with 0.2 Gy (γ-ray, RAD) at 12.5 days post-conception. Co-exposure to BPA and γ-ray induces DNA damage in fetal germ cells in an additive manner, leading to a long-lasting decrease in germ cell abundance. We also observed significant alteration of adult steroidogenesis by RAD exposure independently of the BPA exposure. This is illustrated by the downregulation of steroidogenic genes and the decrease of the number of adult Leydig cells. As a consequence, courtship behavior is modified, and male ultrasonic vocalizations associated with courtship decreased. In conclusion, this study provides evidence for the importance of broadening the concept of endocrine disruptors to include physical agents, leading to a reevaluation of risk management and regulatory decisions.Margaux WieckowskiStéphanie RangaDelphine MoisonSébastien MessiaenSonia AbdallahSylvie GranonRené HabertVirginie Rouiller-FabreGabriel LiveraMarie-Justine GuerquinMDPI AGarticleendocrine disruptorsreproductiongerm cellsLeydig cellsγ-rayBisphenol ABiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11808, p 11808 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endocrine disruptors reproduction germ cells Leydig cells γ-ray Bisphenol A Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	endocrine disruptors reproduction germ cells Leydig cells γ-ray Bisphenol A Biology (General) QH301-705.5 Chemistry QD1-999 Margaux Wieckowski Stéphanie Ranga Delphine Moison Sébastien Messiaen Sonia Abdallah Sylvie Granon René Habert Virginie Rouiller-Fabre Gabriel Livera Marie-Justine Guerquin Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice
description	For decades, numerous chemical pollutants have been described to interfere with endogenous hormone metabolism/signaling altering reproductive functions. Among these endocrine disrupting substances, Bisphenol A (BPA), a widely used compound, is known to negatively impact germ and somatic cells in the testis. Physical agents, such as ionizing radiation, were also described to perturb spermatogenesis. Despite the fact that we are constantly exposed to numerous environmental chemical and physical compounds, very few studies explore the impact of combined exposure to chemical and physical pollutants on reproductive health. The aim of this study was to describe the impact of fetal co-exposure to BPA and IR on testicular function in mice. We exposed pregnant mice to 10 µM BPA (corresponding to 0.5 mg/kg/day) in drinking water from 10.5 dpc until birth, and we irradiated mice with 0.2 Gy (γ-ray, RAD) at 12.5 days post-conception. Co-exposure to BPA and γ-ray induces DNA damage in fetal germ cells in an additive manner, leading to a long-lasting decrease in germ cell abundance. We also observed significant alteration of adult steroidogenesis by RAD exposure independently of the BPA exposure. This is illustrated by the downregulation of steroidogenic genes and the decrease of the number of adult Leydig cells. As a consequence, courtship behavior is modified, and male ultrasonic vocalizations associated with courtship decreased. In conclusion, this study provides evidence for the importance of broadening the concept of endocrine disruptors to include physical agents, leading to a reevaluation of risk management and regulatory decisions.
format	article
author	Margaux Wieckowski Stéphanie Ranga Delphine Moison Sébastien Messiaen Sonia Abdallah Sylvie Granon René Habert Virginie Rouiller-Fabre Gabriel Livera Marie-Justine Guerquin
author_facet	Margaux Wieckowski Stéphanie Ranga Delphine Moison Sébastien Messiaen Sonia Abdallah Sylvie Granon René Habert Virginie Rouiller-Fabre Gabriel Livera Marie-Justine Guerquin
author_sort	Margaux Wieckowski
title	Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice
title_short	Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice
title_full	Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice
title_fullStr	Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice
title_full_unstemmed	Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice
title_sort	unexpected interacting effects of physical (radiation) and chemical (bisphenol a) treatments on male reproductive functions in mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bafdb47791c44049ab2d5a94b133ba09
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Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice

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