MDA-5 recognition of a murine norovirus.

Noroviruses are important human pathogens responsible for most cases of viral epidemic gastroenteritis worldwide. Murine norovirus-1 (MNV-1) is one of several murine noroviruses isolated from research mouse facilities and has been used as a model of human norovirus infection. MNV-1 infection has bee...

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Autores principales:	Stephen A McCartney, Larissa B Thackray, Leonid Gitlin, Susan Gilfillan, Herbert W Virgin, Marco Colonna
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bb03f94d78a9418b89401e29e4d4c8d5
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spelling	oai:doaj.org-article:bb03f94d78a9418b89401e29e4d4c8d52021-11-25T05:46:30ZMDA-5 recognition of a murine norovirus.1553-73661553-737410.1371/journal.ppat.1000108https://doaj.org/article/bb03f94d78a9418b89401e29e4d4c8d52008-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18636103/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Noroviruses are important human pathogens responsible for most cases of viral epidemic gastroenteritis worldwide. Murine norovirus-1 (MNV-1) is one of several murine noroviruses isolated from research mouse facilities and has been used as a model of human norovirus infection. MNV-1 infection has been shown to require components of innate and adaptive immunity for clearance; however, the initial host protein that recognizes MNV-1 infection is unknown. Because noroviruses are RNA viruses, we investigated whether MDA5 and TLR3, cellular sensors that recognize dsRNA, are important for the host response to MNV-1. We demonstrate that MDA5-/- dendritic cells(DC) have a defect in cytokine response to MNV-1. In addition, MNV-1 replicates to higher levels in MDA5-/- DCs as well as in MDA5-/- mice in vivo. Interestingly, TLR3-/- DCs do not have a defect in vitro, but TLR3-/- mice have a slight increase in viral titers. This is the first demonstration of an innate immune sensor for norovirus and shows that MDA5 is required for the control of MNV-1 infection. Knowledge of the host response to MNV-1 may provide keys for prevention and treatment of the human disease.Stephen A McCartneyLarissa B ThackrayLeonid GitlinSusan GilfillanHerbert W VirginMarco ColonnaPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 4, Iss 7, p e1000108 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Stephen A McCartney Larissa B Thackray Leonid Gitlin Susan Gilfillan Herbert W Virgin Marco Colonna MDA-5 recognition of a murine norovirus.
description	Noroviruses are important human pathogens responsible for most cases of viral epidemic gastroenteritis worldwide. Murine norovirus-1 (MNV-1) is one of several murine noroviruses isolated from research mouse facilities and has been used as a model of human norovirus infection. MNV-1 infection has been shown to require components of innate and adaptive immunity for clearance; however, the initial host protein that recognizes MNV-1 infection is unknown. Because noroviruses are RNA viruses, we investigated whether MDA5 and TLR3, cellular sensors that recognize dsRNA, are important for the host response to MNV-1. We demonstrate that MDA5-/- dendritic cells(DC) have a defect in cytokine response to MNV-1. In addition, MNV-1 replicates to higher levels in MDA5-/- DCs as well as in MDA5-/- mice in vivo. Interestingly, TLR3-/- DCs do not have a defect in vitro, but TLR3-/- mice have a slight increase in viral titers. This is the first demonstration of an innate immune sensor for norovirus and shows that MDA5 is required for the control of MNV-1 infection. Knowledge of the host response to MNV-1 may provide keys for prevention and treatment of the human disease.
format	article
author	Stephen A McCartney Larissa B Thackray Leonid Gitlin Susan Gilfillan Herbert W Virgin Marco Colonna
author_facet	Stephen A McCartney Larissa B Thackray Leonid Gitlin Susan Gilfillan Herbert W Virgin Marco Colonna
author_sort	Stephen A McCartney
title	MDA-5 recognition of a murine norovirus.
title_short	MDA-5 recognition of a murine norovirus.
title_full	MDA-5 recognition of a murine norovirus.
title_fullStr	MDA-5 recognition of a murine norovirus.
title_full_unstemmed	MDA-5 recognition of a murine norovirus.
title_sort	mda-5 recognition of a murine norovirus.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/bb03f94d78a9418b89401e29e4d4c8d5
work_keys_str_mv	AT stephenamccartney mda5recognitionofamurinenorovirus AT larissabthackray mda5recognitionofamurinenorovirus AT leonidgitlin mda5recognitionofamurinenorovirus AT susangilfillan mda5recognitionofamurinenorovirus AT herbertwvirgin mda5recognitionofamurinenorovirus AT marcocolonna mda5recognitionofamurinenorovirus
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MDA-5 recognition of a murine norovirus.

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