Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants

Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, posse...

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Autores principales:	Youbin Li, Yuzhe Liu, Haotian Bai, Ronghang Li, Jing Shang, Zhengqing Zhu, Liwei Zhu, Chenyi Zhu, Zhenjia Che, Jincheng Wang, He Liu, Lanfeng Huang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	3D-printed porous titanium alloy scaffold VEGF angiogenesis bioactive interface osseointegration Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/bb060d6843504f158c9e80012a53e10b
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spelling	oai:doaj.org-article:bb060d6843504f158c9e80012a53e10b2021-11-15T06:11:38ZSustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants2296-418510.3389/fbioe.2021.757767https://doaj.org/article/bb060d6843504f158c9e80012a53e10b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.757767/fullhttps://doaj.org/toc/2296-4185Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration.Youbin LiYoubin LiYuzhe LiuYuzhe LiuHaotian BaiRonghang LiRonghang LiJing ShangJing ShangZhengqing ZhuZhengqing ZhuLiwei ZhuLiwei ZhuChenyi ZhuChenyi ZhuZhenjia CheZhenjia CheJincheng WangJincheng WangHe LiuHe LiuLanfeng HuangFrontiers Media S.A.article3D-printed porous titanium alloy scaffoldVEGFangiogenesisbioactive interfaceosseointegrationBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	3D-printed porous titanium alloy scaffold VEGF angiogenesis bioactive interface osseointegration Biotechnology TP248.13-248.65
spellingShingle	3D-printed porous titanium alloy scaffold VEGF angiogenesis bioactive interface osseointegration Biotechnology TP248.13-248.65 Youbin Li Youbin Li Yuzhe Liu Yuzhe Liu Haotian Bai Ronghang Li Ronghang Li Jing Shang Jing Shang Zhengqing Zhu Zhengqing Zhu Liwei Zhu Liwei Zhu Chenyi Zhu Chenyi Zhu Zhenjia Che Zhenjia Che Jincheng Wang Jincheng Wang He Liu He Liu Lanfeng Huang Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
description	Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration.
format	article
author	Youbin Li Youbin Li Yuzhe Liu Yuzhe Liu Haotian Bai Ronghang Li Ronghang Li Jing Shang Jing Shang Zhengqing Zhu Zhengqing Zhu Liwei Zhu Liwei Zhu Chenyi Zhu Chenyi Zhu Zhenjia Che Zhenjia Che Jincheng Wang Jincheng Wang He Liu He Liu Lanfeng Huang
author_facet	Youbin Li Youbin Li Yuzhe Liu Yuzhe Liu Haotian Bai Ronghang Li Ronghang Li Jing Shang Jing Shang Zhengqing Zhu Zhengqing Zhu Liwei Zhu Liwei Zhu Chenyi Zhu Chenyi Zhu Zhenjia Che Zhenjia Che Jincheng Wang Jincheng Wang He Liu He Liu Lanfeng Huang
author_sort	Youbin Li
title	Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_short	Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_full	Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_fullStr	Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_full_unstemmed	Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants
title_sort	sustained release of vegf to promote angiogenesis and osteointegration of three-dimensional printed biomimetic titanium alloy implants
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/bb060d6843504f158c9e80012a53e10b
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Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants

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