Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.

DNA repair mechanisms are essential for preservation of genome integrity. However, it is not clear how DNA are selected and processed at broken ends by exonucleases during repair pathways. Here we show that the DnaQ-like exonuclease RNase T is critical for Escherichia coli resistance to various DNA-...

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Autores principales:	Yu-Yuan Hsiao, Woei-Horng Fang, Chia-Chia Lee, Yi-Ping Chen, Hanna S Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bb08ec8882724f25930b0f0b784c269c
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spelling	oai:doaj.org-article:bb08ec8882724f25930b0f0b784c269c2021-11-18T05:37:33ZStructural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.1544-91731545-788510.1371/journal.pbio.1001803https://doaj.org/article/bb08ec8882724f25930b0f0b784c269c2014-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24594808/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885DNA repair mechanisms are essential for preservation of genome integrity. However, it is not clear how DNA are selected and processed at broken ends by exonucleases during repair pathways. Here we show that the DnaQ-like exonuclease RNase T is critical for Escherichia coli resistance to various DNA-damaging agents and UV radiation. RNase T specifically trims the 3' end of structured DNA, including bulge, bubble, and Y-structured DNA, and it can work with Endonuclease V to restore the deaminated base in an inosine-containing heteroduplex DNA. Crystal structure analyses further reveal how RNase T recognizes the bulge DNA by inserting a phenylalanine into the bulge, and as a result the 3' end of blunt-end bulge DNA can be digested by RNase T. In contrast, the homodimeric RNase T interacts with the Y-structured DNA by a different binding mode via a single protomer so that the 3' overhang of the Y-structured DNA can be trimmed closely to the duplex region. Our data suggest that RNase T likely processes bulge and bubble DNA in the Endonuclease V-dependent DNA repair, whereas it processes Y-structured DNA in UV-induced and various other DNA repair pathways. This study thus provides mechanistic insights for RNase T and thousands of DnaQ-like exonucleases in DNA 3'-end processing.Yu-Yuan HsiaoWoei-Horng FangChia-Chia LeeYi-Ping ChenHanna S YuanPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 12, Iss 3, p e1001803 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Yu-Yuan Hsiao Woei-Horng Fang Chia-Chia Lee Yi-Ping Chen Hanna S Yuan Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.
description	DNA repair mechanisms are essential for preservation of genome integrity. However, it is not clear how DNA are selected and processed at broken ends by exonucleases during repair pathways. Here we show that the DnaQ-like exonuclease RNase T is critical for Escherichia coli resistance to various DNA-damaging agents and UV radiation. RNase T specifically trims the 3' end of structured DNA, including bulge, bubble, and Y-structured DNA, and it can work with Endonuclease V to restore the deaminated base in an inosine-containing heteroduplex DNA. Crystal structure analyses further reveal how RNase T recognizes the bulge DNA by inserting a phenylalanine into the bulge, and as a result the 3' end of blunt-end bulge DNA can be digested by RNase T. In contrast, the homodimeric RNase T interacts with the Y-structured DNA by a different binding mode via a single protomer so that the 3' overhang of the Y-structured DNA can be trimmed closely to the duplex region. Our data suggest that RNase T likely processes bulge and bubble DNA in the Endonuclease V-dependent DNA repair, whereas it processes Y-structured DNA in UV-induced and various other DNA repair pathways. This study thus provides mechanistic insights for RNase T and thousands of DnaQ-like exonucleases in DNA 3'-end processing.
format	article
author	Yu-Yuan Hsiao Woei-Horng Fang Chia-Chia Lee Yi-Ping Chen Hanna S Yuan
author_facet	Yu-Yuan Hsiao Woei-Horng Fang Chia-Chia Lee Yi-Ping Chen Hanna S Yuan
author_sort	Yu-Yuan Hsiao
title	Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.
title_short	Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.
title_full	Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.
title_fullStr	Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.
title_full_unstemmed	Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.
title_sort	structural insights into dna repair by rnase t--an exonuclease processing 3' end of structured dna in repair pathways.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bb08ec8882724f25930b0f0b784c269c
work_keys_str_mv	AT yuyuanhsiao structuralinsightsintodnarepairbyrnasetanexonucleaseprocessing3endofstructureddnainrepairpathways AT woeihorngfang structuralinsightsintodnarepairbyrnasetanexonucleaseprocessing3endofstructureddnainrepairpathways AT chiachialee structuralinsightsintodnarepairbyrnasetanexonucleaseprocessing3endofstructureddnainrepairpathways AT yipingchen structuralinsightsintodnarepairbyrnasetanexonucleaseprocessing3endofstructureddnainrepairpathways AT hannasyuan structuralinsightsintodnarepairbyrnasetanexonucleaseprocessing3endofstructureddnainrepairpathways
_version_	1718424876003360768

Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.

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