Histone deacetylase 3 is required for iNKT cell development

Abstract NKT cells are a distinct subset that have developmental requirements that often differ from conventional T cells. Here, we show that NKT-specific deletion of Hdac3 results in a severe reduction in the number of iNKT cells, particularly of NKT1 cells. In addition, there is decreased cytokine...

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Autores principales:	Puspa Thapa, Sinibaldo Romero Arocha, Ji Young Chung, Derek B. Sant’Angelo, Virginia Smith Shapiro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb0b26a9f24f4d3983eb28ce34699aa4
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spelling	oai:doaj.org-article:bb0b26a9f24f4d3983eb28ce34699aa42021-12-02T15:06:17ZHistone deacetylase 3 is required for iNKT cell development10.1038/s41598-017-06102-52045-2322https://doaj.org/article/bb0b26a9f24f4d3983eb28ce34699aa42017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06102-5https://doaj.org/toc/2045-2322Abstract NKT cells are a distinct subset that have developmental requirements that often differ from conventional T cells. Here, we show that NKT-specific deletion of Hdac3 results in a severe reduction in the number of iNKT cells, particularly of NKT1 cells. In addition, there is decreased cytokine production by Hdac3-deficient NKT2 and NKT17 cells. Hdac3-deficient iNKT cells have increased cell death that is not rescued by transgenic expression of Bcl-2 or Bcl-xL. Hdac3-deficient iNKT cells have less Cyto-ID staining and lower LC3A/B expression, indicative of reduced autophagy. Interestingly, Hdac3-deficient iNKT cells also have lower expression of the nutrient receptors GLUT1, CD71 and CD98, which would increase the need for autophagy when nutrients are limiting. Therefore, Hdac3 is required for iNKT cell development and differentiation.Puspa ThapaSinibaldo Romero ArochaJi Young ChungDerek B. Sant’AngeloVirginia Smith ShapiroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Puspa Thapa Sinibaldo Romero Arocha Ji Young Chung Derek B. Sant’Angelo Virginia Smith Shapiro Histone deacetylase 3 is required for iNKT cell development
description	Abstract NKT cells are a distinct subset that have developmental requirements that often differ from conventional T cells. Here, we show that NKT-specific deletion of Hdac3 results in a severe reduction in the number of iNKT cells, particularly of NKT1 cells. In addition, there is decreased cytokine production by Hdac3-deficient NKT2 and NKT17 cells. Hdac3-deficient iNKT cells have increased cell death that is not rescued by transgenic expression of Bcl-2 or Bcl-xL. Hdac3-deficient iNKT cells have less Cyto-ID staining and lower LC3A/B expression, indicative of reduced autophagy. Interestingly, Hdac3-deficient iNKT cells also have lower expression of the nutrient receptors GLUT1, CD71 and CD98, which would increase the need for autophagy when nutrients are limiting. Therefore, Hdac3 is required for iNKT cell development and differentiation.
format	article
author	Puspa Thapa Sinibaldo Romero Arocha Ji Young Chung Derek B. Sant’Angelo Virginia Smith Shapiro
author_facet	Puspa Thapa Sinibaldo Romero Arocha Ji Young Chung Derek B. Sant’Angelo Virginia Smith Shapiro
author_sort	Puspa Thapa
title	Histone deacetylase 3 is required for iNKT cell development
title_short	Histone deacetylase 3 is required for iNKT cell development
title_full	Histone deacetylase 3 is required for iNKT cell development
title_fullStr	Histone deacetylase 3 is required for iNKT cell development
title_full_unstemmed	Histone deacetylase 3 is required for iNKT cell development
title_sort	histone deacetylase 3 is required for inkt cell development
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bb0b26a9f24f4d3983eb28ce34699aa4
work_keys_str_mv	AT puspathapa histonedeacetylase3isrequiredforinktcelldevelopment AT sinibaldoromeroarocha histonedeacetylase3isrequiredforinktcelldevelopment AT jiyoungchung histonedeacetylase3isrequiredforinktcelldevelopment AT derekbsantangelo histonedeacetylase3isrequiredforinktcelldevelopment AT virginiasmithshapiro histonedeacetylase3isrequiredforinktcelldevelopment
_version_	1718388539017658368

Histone deacetylase 3 is required for iNKT cell development

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