Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA

Abstract. Roesma DI, Tjong DH, Janra MN, Aidil DR. 2021. Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA. Biodiversitas 22: 2794-2802. Environmental DNA (eDNA) is one of the DNA sources without directly disturbing the organisms. The eDNA potentiall...

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Autores principales:	DEWI IMELDA ROESMA, HON TJONG DJONG, MUHAMMAD NAZRI JANRA, DYTA RABBANI AIDIL
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	environmental dna maninjau lake next-generation sequencing vertebrates Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bb0c1ae40266406d90fd4aed8cb9afd9
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spelling	oai:doaj.org-article:bb0c1ae40266406d90fd4aed8cb9afd92021-11-22T12:13:35ZFreshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA1412-033X2085-472210.13057/biodiv/d220543https://doaj.org/article/bb0c1ae40266406d90fd4aed8cb9afd92021-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8185https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Roesma DI, Tjong DH, Janra MN, Aidil DR. 2021. Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA. Biodiversitas 22: 2794-2802. Environmental DNA (eDNA) is one of the DNA sources without directly disturbing the organisms. The eDNA potentially an effective method with next-generation sequencing (NGS) that can read all DNA in parallel at one sequential run. Maninjau Lake in West Sumatra has experienced a decline the species richness, thus monitor biodiversity with the non-invasive method is needed. This study aimed to apply eDNA for monitoring freshwater vertebrates in Maninjau Lake. The eDNA was collected from water samples using the filter membrane to DNA isolation and sequencing with the NGS technique from Illumina. The eDNA method detected 150 vertebrate individuals and consists of 92 Actinopterygii fishes, three chondrichthyes fishes, 11 amphibians, seven reptiles, ten aves, and 27 mammals. Among all individuals were detected, only 11% known present in Maninjau Lake, while the others (89%) are native species to other countries. The fishes were detected living in Maninjau Lake by the eDNA method, i.e., Cyprinidae (Carassius auratus and Cyprinus carpio), Cichlidae (Oreochromis niloticus), and Poeciliidae (Gambusia affinis and Xiphophorus maculatus). Bias results in this study to be influenced by various factors i.e., possibility of contamination, poor DNA quality, the bias results during molecular processes, and not available the complete sequences databases to assigned into the correct taxa, especially fish species in Maninjau Lake. The eDNA method for monitoring biodiversity should consider the increase of sampling sites, replication samples, and the availability of sequences database in Genbank, NCBIDEWI IMELDA ROESMAHON TJONG DJONGMUHAMMAD NAZRI JANRADYTA RABBANI AIDILMBI & UNS Soloarticleenvironmental dnamaninjau lakenext-generation sequencingvertebratesBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	environmental dna maninjau lake next-generation sequencing vertebrates Biology (General) QH301-705.5
spellingShingle	environmental dna maninjau lake next-generation sequencing vertebrates Biology (General) QH301-705.5 DEWI IMELDA ROESMA HON TJONG DJONG MUHAMMAD NAZRI JANRA DYTA RABBANI AIDIL Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA
description	Abstract. Roesma DI, Tjong DH, Janra MN, Aidil DR. 2021. Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA. Biodiversitas 22: 2794-2802. Environmental DNA (eDNA) is one of the DNA sources without directly disturbing the organisms. The eDNA potentially an effective method with next-generation sequencing (NGS) that can read all DNA in parallel at one sequential run. Maninjau Lake in West Sumatra has experienced a decline the species richness, thus monitor biodiversity with the non-invasive method is needed. This study aimed to apply eDNA for monitoring freshwater vertebrates in Maninjau Lake. The eDNA was collected from water samples using the filter membrane to DNA isolation and sequencing with the NGS technique from Illumina. The eDNA method detected 150 vertebrate individuals and consists of 92 Actinopterygii fishes, three chondrichthyes fishes, 11 amphibians, seven reptiles, ten aves, and 27 mammals. Among all individuals were detected, only 11% known present in Maninjau Lake, while the others (89%) are native species to other countries. The fishes were detected living in Maninjau Lake by the eDNA method, i.e., Cyprinidae (Carassius auratus and Cyprinus carpio), Cichlidae (Oreochromis niloticus), and Poeciliidae (Gambusia affinis and Xiphophorus maculatus). Bias results in this study to be influenced by various factors i.e., possibility of contamination, poor DNA quality, the bias results during molecular processes, and not available the complete sequences databases to assigned into the correct taxa, especially fish species in Maninjau Lake. The eDNA method for monitoring biodiversity should consider the increase of sampling sites, replication samples, and the availability of sequences database in Genbank, NCBI
format	article
author	DEWI IMELDA ROESMA HON TJONG DJONG MUHAMMAD NAZRI JANRA DYTA RABBANI AIDIL
author_facet	DEWI IMELDA ROESMA HON TJONG DJONG MUHAMMAD NAZRI JANRA DYTA RABBANI AIDIL
author_sort	DEWI IMELDA ROESMA
title	Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA
title_short	Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA
title_full	Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA
title_fullStr	Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA
title_full_unstemmed	Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA
title_sort	freshwater vertebrates monitoring in maninjau lake, west sumatra, indonesia using environmental dna
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/bb0c1ae40266406d90fd4aed8cb9afd9
work_keys_str_mv	AT dewiimeldaroesma freshwatervertebratesmonitoringinmaninjaulakewestsumatraindonesiausingenvironmentaldna AT hontjongdjong freshwatervertebratesmonitoringinmaninjaulakewestsumatraindonesiausingenvironmentaldna AT muhammadnazrijanra freshwatervertebratesmonitoringinmaninjaulakewestsumatraindonesiausingenvironmentaldna AT dytarabbaniaidil freshwatervertebratesmonitoringinmaninjaulakewestsumatraindonesiausingenvironmentaldna
_version_	1718417698473377792

Freshwater vertebrates monitoring in Maninjau Lake, West Sumatra, Indonesia using environmental DNA

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