Heat impact during laser ablation extraction of mineralised tissue micropillars

Abstract The underlying constraint of ultrashort pulsed laser ablation in both the clinical and micromachining setting is the uncertainty regarding the impact on the composition of material surrounding the ablated region. A heat model representing the laser-tissue interaction was implemented into a...

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Autores principales:	Samuel McPhee, Alexander Groetsch, Jonathan D. Shephard, Uwe Wolfram
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb0fb53550c640aa969b643d24757abf
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spelling	oai:doaj.org-article:bb0fb53550c640aa969b643d24757abf2021-12-02T16:53:12ZHeat impact during laser ablation extraction of mineralised tissue micropillars10.1038/s41598-021-89181-92045-2322https://doaj.org/article/bb0fb53550c640aa969b643d24757abf2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89181-9https://doaj.org/toc/2045-2322Abstract The underlying constraint of ultrashort pulsed laser ablation in both the clinical and micromachining setting is the uncertainty regarding the impact on the composition of material surrounding the ablated region. A heat model representing the laser-tissue interaction was implemented into a finite element suite to assess the cumulative temperature response of bone during ultrashort pulsed laser ablation. As an example, we focus on the extraction of mineralised collagen fibre micropillars. Laser induced heating can cause denaturation of the collagen, resulting in ultrastructural loss which could affect mechanical testing results. Laser parameters were taken from a used micropillar extraction protocol. The laser scanning pattern consisted of 4085 pulses, with a final radial pass being 22 $$\upmu {\text {m}}$$ μ m away from the micropillar. The micropillar temperature was elevated to 70.58 $$^{\circ }{\text {C}}$$ ∘ C , remaining 79.42 $$^{\circ }{\text {C}}$$ ∘ C lower than that of which we interpret as an onset for denaturation. We verified the results by means of Raman microscopy and Energy Dispersive X-ray Microanalysis and found the laser-material interaction had no effect on the collagen molecules or mineral nanocrystals that constitute the micropillars. We, thus, show that ultrashort pulsed laser ablation is a safe and viable tool to fabricate bone specimens for mechanical testing at the micro- and nanoscale and we provide a computational model to efficiently assess this.Samuel McPheeAlexander GroetschJonathan D. ShephardUwe WolframNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samuel McPhee Alexander Groetsch Jonathan D. Shephard Uwe Wolfram Heat impact during laser ablation extraction of mineralised tissue micropillars
description	Abstract The underlying constraint of ultrashort pulsed laser ablation in both the clinical and micromachining setting is the uncertainty regarding the impact on the composition of material surrounding the ablated region. A heat model representing the laser-tissue interaction was implemented into a finite element suite to assess the cumulative temperature response of bone during ultrashort pulsed laser ablation. As an example, we focus on the extraction of mineralised collagen fibre micropillars. Laser induced heating can cause denaturation of the collagen, resulting in ultrastructural loss which could affect mechanical testing results. Laser parameters were taken from a used micropillar extraction protocol. The laser scanning pattern consisted of 4085 pulses, with a final radial pass being 22 $$\upmu {\text {m}}$$ μ m away from the micropillar. The micropillar temperature was elevated to 70.58 $$^{\circ }{\text {C}}$$ ∘ C , remaining 79.42 $$^{\circ }{\text {C}}$$ ∘ C lower than that of which we interpret as an onset for denaturation. We verified the results by means of Raman microscopy and Energy Dispersive X-ray Microanalysis and found the laser-material interaction had no effect on the collagen molecules or mineral nanocrystals that constitute the micropillars. We, thus, show that ultrashort pulsed laser ablation is a safe and viable tool to fabricate bone specimens for mechanical testing at the micro- and nanoscale and we provide a computational model to efficiently assess this.
format	article
author	Samuel McPhee Alexander Groetsch Jonathan D. Shephard Uwe Wolfram
author_facet	Samuel McPhee Alexander Groetsch Jonathan D. Shephard Uwe Wolfram
author_sort	Samuel McPhee
title	Heat impact during laser ablation extraction of mineralised tissue micropillars
title_short	Heat impact during laser ablation extraction of mineralised tissue micropillars
title_full	Heat impact during laser ablation extraction of mineralised tissue micropillars
title_fullStr	Heat impact during laser ablation extraction of mineralised tissue micropillars
title_full_unstemmed	Heat impact during laser ablation extraction of mineralised tissue micropillars
title_sort	heat impact during laser ablation extraction of mineralised tissue micropillars
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bb0fb53550c640aa969b643d24757abf
work_keys_str_mv	AT samuelmcphee heatimpactduringlaserablationextractionofmineralisedtissuemicropillars AT alexandergroetsch heatimpactduringlaserablationextractionofmineralisedtissuemicropillars AT jonathandshephard heatimpactduringlaserablationextractionofmineralisedtissuemicropillars AT uwewolfram heatimpactduringlaserablationextractionofmineralisedtissuemicropillars
_version_	1718382882886516736

Heat impact during laser ablation extraction of mineralised tissue micropillars

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