Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?

<h4>Background</h4>The assessment of blood lipids is very frequent in clinical research as it is assumed to reflect the lipid composition of peripheral tissues. Even well accepted such relationships have never been clearly established. This is particularly true in ophthalmology where the...

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Autores principales:	Niyazi Acar, Olivier Berdeaux, Stéphane Grégoire, Stéphanie Cabaret, Lucy Martine, Philippe Gain, Gilles Thuret, Catherine P Creuzot-Garcher, Alain M Bron, Lionel Bretillon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb19dabff2c14f01a291ce50b90f8032
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spelling	oai:doaj.org-article:bb19dabff2c14f01a291ce50b90f80322021-11-18T07:22:52ZLipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?1932-620310.1371/journal.pone.0035102https://doaj.org/article/bb19dabff2c14f01a291ce50b90f80322012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22496896/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The assessment of blood lipids is very frequent in clinical research as it is assumed to reflect the lipid composition of peripheral tissues. Even well accepted such relationships have never been clearly established. This is particularly true in ophthalmology where the use of blood lipids has become very common following recent data linking lipid intake to ocular health and disease. In the present study, we wanted to determine in humans whether a lipidomic approach based on red blood cells could reveal associations between circulating and tissue lipid profiles. To check if the analytical sensitivity may be of importance in such analyses, we have used a double approach for lipidomics.<h4>Methodology and principal findings</h4>Red blood cells, retinas and optic nerves were collected from 9 human donors. The lipidomic analyses on tissues consisted in gas chromatography and liquid chromatography coupled to an electrospray ionization source-mass spectrometer (LC-ESI-MS). Gas chromatography did not reveal any relevant association between circulating and ocular fatty acids except for arachidonic acid whose circulating amounts were positively associated with its levels in the retina and in the optic nerve. In contrast, several significant associations emerged from LC-ESI-MS analyses. Particularly, lipid entities in red blood cells were positively or negatively associated with representative pools of retinal docosahexaenoic acid (DHA), retinal very-long chain polyunsaturated fatty acids (VLC-PUFA) or optic nerve plasmalogens.<h4>Conclusions and significance</h4>LC-ESI-MS is more appropriate than gas chromatography for lipidomics on red blood cells, and further extrapolation to ocular lipids. The several individual lipid species we have identified are good candidates to represent circulating biomarkers of ocular lipids. However, further investigation is needed before considering them as indexes of disease risk and before using them in clinical studies on optic nerve neuropathies or retinal diseases displaying photoreceptors degeneration.Niyazi AcarOlivier BerdeauxStéphane GrégoireStéphanie CabaretLucy MartinePhilippe GainGilles ThuretCatherine P Creuzot-GarcherAlain M BronLionel BretillonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35102 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Niyazi Acar Olivier Berdeaux Stéphane Grégoire Stéphanie Cabaret Lucy Martine Philippe Gain Gilles Thuret Catherine P Creuzot-Garcher Alain M Bron Lionel Bretillon Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?
description	<h4>Background</h4>The assessment of blood lipids is very frequent in clinical research as it is assumed to reflect the lipid composition of peripheral tissues. Even well accepted such relationships have never been clearly established. This is particularly true in ophthalmology where the use of blood lipids has become very common following recent data linking lipid intake to ocular health and disease. In the present study, we wanted to determine in humans whether a lipidomic approach based on red blood cells could reveal associations between circulating and tissue lipid profiles. To check if the analytical sensitivity may be of importance in such analyses, we have used a double approach for lipidomics.<h4>Methodology and principal findings</h4>Red blood cells, retinas and optic nerves were collected from 9 human donors. The lipidomic analyses on tissues consisted in gas chromatography and liquid chromatography coupled to an electrospray ionization source-mass spectrometer (LC-ESI-MS). Gas chromatography did not reveal any relevant association between circulating and ocular fatty acids except for arachidonic acid whose circulating amounts were positively associated with its levels in the retina and in the optic nerve. In contrast, several significant associations emerged from LC-ESI-MS analyses. Particularly, lipid entities in red blood cells were positively or negatively associated with representative pools of retinal docosahexaenoic acid (DHA), retinal very-long chain polyunsaturated fatty acids (VLC-PUFA) or optic nerve plasmalogens.<h4>Conclusions and significance</h4>LC-ESI-MS is more appropriate than gas chromatography for lipidomics on red blood cells, and further extrapolation to ocular lipids. The several individual lipid species we have identified are good candidates to represent circulating biomarkers of ocular lipids. However, further investigation is needed before considering them as indexes of disease risk and before using them in clinical studies on optic nerve neuropathies or retinal diseases displaying photoreceptors degeneration.
format	article
author	Niyazi Acar Olivier Berdeaux Stéphane Grégoire Stéphanie Cabaret Lucy Martine Philippe Gain Gilles Thuret Catherine P Creuzot-Garcher Alain M Bron Lionel Bretillon
author_facet	Niyazi Acar Olivier Berdeaux Stéphane Grégoire Stéphanie Cabaret Lucy Martine Philippe Gain Gilles Thuret Catherine P Creuzot-Garcher Alain M Bron Lionel Bretillon
author_sort	Niyazi Acar
title	Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?
title_short	Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?
title_full	Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?
title_fullStr	Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?
title_full_unstemmed	Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?
title_sort	lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/bb19dabff2c14f01a291ce50b90f8032
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Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?

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