Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes

Huichuan Duan,1,* Bei Feng,2,* Xiangkai Guo,3 Jiaming Wang,1 Li Zhao,1 Guangdong Zhou,1 Wei Liu,1 Yilin Cao,1 Wen Jie Zhang1 1Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissu...

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Autores principales:	Duan H, Feng B, Guo X, Wang J, Zhao L, Zhou G, Liu W, Cao Y, Zhang WJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bb1f2676f1fd42afb984f1ccd611edba
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spelling	oai:doaj.org-article:bb1f2676f1fd42afb984f1ccd611edba2021-12-02T02:49:07ZEngineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes1176-91141178-2013https://doaj.org/article/bb1f2676f1fd42afb984f1ccd611edba2013-06-01T00:00:00Zhttp://www.dovepress.com/engineering-of-epidermis-skin-grafts-using-electrospun-nanofibrous-gel-a13257https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Huichuan Duan,1,* Bei Feng,2,* Xiangkai Guo,3 Jiaming Wang,1 Li Zhao,1 Guangdong Zhou,1 Wei Liu,1 Yilin Cao,1 Wen Jie Zhang1 1Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai, People’s Republic of China; 2Department of Cardiothoracic Surgery, Institute of Pediatric Translational Medicine, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Ji Nan, Shan Dong, People’s Republic of China *These authors contributed equally to this work Abstract: Skin engineering provides a new strategy for treating a wide variety of skin defects. In particular, electrospun nanofibrous membranes have been used as carriers for epidermis engineering. The aim of this study was to investigate the feasibility of a modified gelatin and polycaprolactone (GT/PCL) electrospun membrane for epidermis engineering. The biocompatibility of the membranes was evaluated by seeding HaCaT cells (human keratinocyte cell line) on the membrane and the mechanical properties of the membranes were determined with and without cells after culture. A cell proliferation assay showing that HaCaT cells attached and proliferated well on the membranes demonstrated that the membranes possess good biocompatibility. Mechanical tests showed that the membranes are strong enough to be handled during transplantation. Further in vivo transplantation studies revealed that epidermises engineered with GT/PCL membranes were able to repair skin defects in the nude mouse. These results demonstrate that GT/PCL electrospun membranes could be suitable scaffolds for skin engineering. Keywords: epidermis engineering, electrospun nanofibrous membrane, gelatin, polycaprolactoneDuan HFeng BGuo XWang JZhao LZhou GLiu WCao YZhang WJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2077-2084 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Duan H Feng B Guo X Wang J Zhao L Zhou G Liu W Cao Y Zhang WJ Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes
description	Huichuan Duan,1,* Bei Feng,2,* Xiangkai Guo,3 Jiaming Wang,1 Li Zhao,1 Guangdong Zhou,1 Wei Liu,1 Yilin Cao,1 Wen Jie Zhang1 1Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai, People’s Republic of China; 2Department of Cardiothoracic Surgery, Institute of Pediatric Translational Medicine, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Ji Nan, Shan Dong, People’s Republic of China *These authors contributed equally to this work Abstract: Skin engineering provides a new strategy for treating a wide variety of skin defects. In particular, electrospun nanofibrous membranes have been used as carriers for epidermis engineering. The aim of this study was to investigate the feasibility of a modified gelatin and polycaprolactone (GT/PCL) electrospun membrane for epidermis engineering. The biocompatibility of the membranes was evaluated by seeding HaCaT cells (human keratinocyte cell line) on the membrane and the mechanical properties of the membranes were determined with and without cells after culture. A cell proliferation assay showing that HaCaT cells attached and proliferated well on the membranes demonstrated that the membranes possess good biocompatibility. Mechanical tests showed that the membranes are strong enough to be handled during transplantation. Further in vivo transplantation studies revealed that epidermises engineered with GT/PCL membranes were able to repair skin defects in the nude mouse. These results demonstrate that GT/PCL electrospun membranes could be suitable scaffolds for skin engineering. Keywords: epidermis engineering, electrospun nanofibrous membrane, gelatin, polycaprolactone
format	article
author	Duan H Feng B Guo X Wang J Zhao L Zhou G Liu W Cao Y Zhang WJ
author_facet	Duan H Feng B Guo X Wang J Zhao L Zhou G Liu W Cao Y Zhang WJ
author_sort	Duan H
title	Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes
title_short	Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes
title_full	Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes
title_fullStr	Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes
title_full_unstemmed	Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes
title_sort	engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/bb1f2676f1fd42afb984f1ccd611edba
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Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes

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