High-depth spatial transcriptome analysis by photo-isolation chemistry

Spatial analysis of RNAseq data is important. Here the authors report a method for transcriptome profiling combined with photo-isolation chemistry to allow determination of expression profiles specifically from photo-irradiated regions of interest which they use in mouse brains and embryonic tissues...

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Auteurs principaux:	Mizuki Honda, Shinya Oki, Ryuichi Kimura, Akihito Harada, Kazumitsu Maehara, Kaori Tanaka, Chikara Meno, Yasuyuki Ohkawa
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/bb1fc3bbebd6485c9ec92dde46e117b8
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id	oai:doaj.org-article:bb1fc3bbebd6485c9ec92dde46e117b8
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spelling	oai:doaj.org-article:bb1fc3bbebd6485c9ec92dde46e117b82021-12-02T16:17:57ZHigh-depth spatial transcriptome analysis by photo-isolation chemistry10.1038/s41467-021-24691-82041-1723https://doaj.org/article/bb1fc3bbebd6485c9ec92dde46e117b82021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24691-8https://doaj.org/toc/2041-1723Spatial analysis of RNAseq data is important. Here the authors report a method for transcriptome profiling combined with photo-isolation chemistry to allow determination of expression profiles specifically from photo-irradiated regions of interest which they use in mouse brains and embryonic tissues.Mizuki HondaShinya OkiRyuichi KimuraAkihito HaradaKazumitsu MaeharaKaori TanakaChikara MenoYasuyuki OhkawaNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mizuki Honda Shinya Oki Ryuichi Kimura Akihito Harada Kazumitsu Maehara Kaori Tanaka Chikara Meno Yasuyuki Ohkawa High-depth spatial transcriptome analysis by photo-isolation chemistry
description	Spatial analysis of RNAseq data is important. Here the authors report a method for transcriptome profiling combined with photo-isolation chemistry to allow determination of expression profiles specifically from photo-irradiated regions of interest which they use in mouse brains and embryonic tissues.
format	article
author	Mizuki Honda Shinya Oki Ryuichi Kimura Akihito Harada Kazumitsu Maehara Kaori Tanaka Chikara Meno Yasuyuki Ohkawa
author_facet	Mizuki Honda Shinya Oki Ryuichi Kimura Akihito Harada Kazumitsu Maehara Kaori Tanaka Chikara Meno Yasuyuki Ohkawa
author_sort	Mizuki Honda
title	High-depth spatial transcriptome analysis by photo-isolation chemistry
title_short	High-depth spatial transcriptome analysis by photo-isolation chemistry
title_full	High-depth spatial transcriptome analysis by photo-isolation chemistry
title_fullStr	High-depth spatial transcriptome analysis by photo-isolation chemistry
title_full_unstemmed	High-depth spatial transcriptome analysis by photo-isolation chemistry
title_sort	high-depth spatial transcriptome analysis by photo-isolation chemistry
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bb1fc3bbebd6485c9ec92dde46e117b8
work_keys_str_mv	AT mizukihonda highdepthspatialtranscriptomeanalysisbyphotoisolationchemistry AT shinyaoki highdepthspatialtranscriptomeanalysisbyphotoisolationchemistry AT ryuichikimura highdepthspatialtranscriptomeanalysisbyphotoisolationchemistry AT akihitoharada highdepthspatialtranscriptomeanalysisbyphotoisolationchemistry AT kazumitsumaehara highdepthspatialtranscriptomeanalysisbyphotoisolationchemistry AT kaoritanaka highdepthspatialtranscriptomeanalysisbyphotoisolationchemistry AT chikarameno highdepthspatialtranscriptomeanalysisbyphotoisolationchemistry AT yasuyukiohkawa highdepthspatialtranscriptomeanalysisbyphotoisolationchemistry
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High-depth spatial transcriptome analysis by photo-isolation chemistry

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