Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses

Abstract Bioactive glasses convert to a biomimetic apatite when in contact with physiological solutions; however, the number and type of phases precipitating depends on glass composition and reactivity. This process is typically followed by X-ray diffraction and infrared spectroscopy. Here, we visua...

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Autores principales:	Altair T. Contreras Jaimes, Gloria Kirste, Araceli de Pablos-Martín, Susanne Selle, Juliana Martins de Souza e Silva, Jonathan Massera, Natalia Karpukhina, Robert G. Hill, Delia S. Brauer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb1fe4d696894eb6a011abd7df996374
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spelling	oai:doaj.org-article:bb1fe4d696894eb6a011abd7df9963742021-12-02T17:37:34ZNano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses10.1038/s41598-021-98863-32045-2322https://doaj.org/article/bb1fe4d696894eb6a011abd7df9963742021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98863-3https://doaj.org/toc/2045-2322Abstract Bioactive glasses convert to a biomimetic apatite when in contact with physiological solutions; however, the number and type of phases precipitating depends on glass composition and reactivity. This process is typically followed by X-ray diffraction and infrared spectroscopy. Here, we visualise surface mineralisation in a series of sodium-free bioactive glasses, using transmission electron microscopy (TEM) with energy-dispersive X-ray spectroscopy (EDXS) and X-ray nano-computed tomography (nano-CT). In the glasses, the phosphate content was increased while adding stoichiometric amounts of calcium to maintain phosphate in an orthophosphate environment in the glass. Calcium fluoride was added to keep the melting temperature low. TEM brought to light the presence of phosphate clustering and nearly crystalline calcium fluoride environments in the glasses. A combination of analytical methods, including solid-state NMR, shows how with increasing phosphate content in the glass, precipitation of calcium fluoride during immersion is superseded by fluorapatite precipitation. Nano-CT gives insight into bioactive glass particle morphology after immersion, while TEM illustrates how compositional changes in the glass affect microstructure at a sub-micron to nanometre-level.Altair T. Contreras JaimesGloria KirsteAraceli de Pablos-MartínSusanne SelleJuliana Martins de Souza e SilvaJonathan MasseraNatalia KarpukhinaRobert G. HillDelia S. BrauerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Altair T. Contreras Jaimes Gloria Kirste Araceli de Pablos-Martín Susanne Selle Juliana Martins de Souza e Silva Jonathan Massera Natalia Karpukhina Robert G. Hill Delia S. Brauer Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses
description	Abstract Bioactive glasses convert to a biomimetic apatite when in contact with physiological solutions; however, the number and type of phases precipitating depends on glass composition and reactivity. This process is typically followed by X-ray diffraction and infrared spectroscopy. Here, we visualise surface mineralisation in a series of sodium-free bioactive glasses, using transmission electron microscopy (TEM) with energy-dispersive X-ray spectroscopy (EDXS) and X-ray nano-computed tomography (nano-CT). In the glasses, the phosphate content was increased while adding stoichiometric amounts of calcium to maintain phosphate in an orthophosphate environment in the glass. Calcium fluoride was added to keep the melting temperature low. TEM brought to light the presence of phosphate clustering and nearly crystalline calcium fluoride environments in the glasses. A combination of analytical methods, including solid-state NMR, shows how with increasing phosphate content in the glass, precipitation of calcium fluoride during immersion is superseded by fluorapatite precipitation. Nano-CT gives insight into bioactive glass particle morphology after immersion, while TEM illustrates how compositional changes in the glass affect microstructure at a sub-micron to nanometre-level.
format	article
author	Altair T. Contreras Jaimes Gloria Kirste Araceli de Pablos-Martín Susanne Selle Juliana Martins de Souza e Silva Jonathan Massera Natalia Karpukhina Robert G. Hill Delia S. Brauer
author_facet	Altair T. Contreras Jaimes Gloria Kirste Araceli de Pablos-Martín Susanne Selle Juliana Martins de Souza e Silva Jonathan Massera Natalia Karpukhina Robert G. Hill Delia S. Brauer
author_sort	Altair T. Contreras Jaimes
title	Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses
title_short	Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses
title_full	Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses
title_fullStr	Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses
title_full_unstemmed	Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses
title_sort	nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bb1fe4d696894eb6a011abd7df996374
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Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses

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